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MP Biomedicals 6004-500, Инструкция по эксплуатации

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®

FastRNA  Pro Isolation Kits 

®

The FastPrep -24 System is also ideal for use in RNA isolation. Several kits are 
available for researchers requiring RNA isolation. These are :

®

•

FastRNA  Pro Blue Kit (Bacteria)

®

•

FastRNA  Pro Red Kit (Yeast)

® 

•

FastRNA Pro Green Kit (Plant/Animal Tissues)

®

•

FastRNA  Pro Soil - Direct Kit

®

•

FastRNA  Pro Soil - Indirect Kit

TM

Each kit includes Lysing Matrix B, C, D or E and proprietary RNApro  solutions. 
These are for single-reagent purification and contain RNA stabilizers. Then the 
researcher just finishes with chloroform extraction and precipitation.

TM TM 

FastPROTEIN Blue and FastPROTEIN Red Matrix

TM TM 

FastPROTEIN

Blue Matrix and FastPROTEIN

Red Matrix are designed for 

bacteria and yeast protein extraction respectively.

These matrices are ideal for optimizing induction conditions and quickly lyse 
samples from a range of conditions and run on a protein gel.
No special buffer is required for lysis. The researcher can use his/her own 

TM 

protein induction media as "lysis buffer". The FastPROTEIN

Red Matrix kit 

includes a lysis buffer named "Yeast Breaking Buffer".

30

www.mpbio.com

Содержание 6004-500

Страница 1: ...www mpbio com MP Biomedicals 29525 Fountain Parkway Solon OH 44139 tel 1 800 854 0530 fax 1 800 334 6999 One Call One Source A World of Biotechnology Reagents Instruction Manual Instruction Manual ...

Страница 2: ...ce the precautionary an operating instructions Know the limitations and hazards associated with using this unit Observe the precautionary and operational decals placed on the unit DO NOT operate this unit in an environment where other devices are being used that intentionally radiate electromagnetic energy in an unshielded manner DO NOT use sharp objects such as a pencil point or ballpoint pen to ...

Страница 3: ...Model 6004 500 www mpbio com ...

Страница 4: ...9 3 2 Preparing the FastPrep 24 for Operation 12 3 3 Programming the FastPrep 24 12 3 4 Save Programs 13 Section IV Specifications 15 Section V Warranty and Liability 16 Section VI Appendices 18 2 1 APPENDIX 1 Maintenance Cleaning 18 2 2 APPENDIX 2 An Explanation of FastPrep 24 Instrument Speed Settings 21 2 3 APPENDIX 3 FastPrep 24 Product Line 22 2 4 APPENDIX 4 Accessories and Spare Parts for Fa...

Страница 5: ...stPrep 24 Instrument lyses thoroughly and quickly any tissues and cells and thus allows easy and reproducible isolation of stable RNA active proteins and full length genomic DNA Samples and buffers are added to a Lysing Matrix tube containing specialized Lysing Matrix beads The ergonomic design of the instrument ensures an easy loading of the sample tubes that remains securely sealed during the pr...

Страница 6: ...nt Should there be any damage report it to the carrier and contact MP Biomedicals immediately Save the packaging material in the event a return is necessary 2 3 Set up To assure safe operation and best result read this manual before operating the FastPrep 24 Instrument The FastPrep 24 Instrument comes fully assembled requiring very little set up Install the system on a clean dry stable surface wit...

Страница 7: ...panel keys are given below Ref Fig 2 1 SET Key Press this key to select Speed Time as displayed on LCD Speed Selectable from 4 0 m s to 6 5 m s In steps of 0 5 m s Default 4 0 m s Tube holder Selectable Time Selectable from I sec to 60 sec in steps of I second Default 20 seconds 2 RUN Key To Start or Stop the Instrument 3 Key To Increase the selected value of speed tube holder time 4 Key To Decrea...

Страница 8: ...ut by rotating counter clockwise until it come out Ref Fig 3 2 Remove the securing knob ratchet Nut Fig 3 3 Take out the assembly of spoke plate and test tube holder Fig 4 Ref Fig 6 9 www mpbio com Securing Knob Ratchet Nut Spoke Plate Three Step Spoke Plate Tube Holder ...

Страница 9: ...ey fit snugly in the holes It is preferred to position the tubes symmetrically 6 When all the sample tubes are loaded place this assembly into the FastPrep 24 Instrument Align the locking pin of Aluminium three steps with the holes under the Tube Holder ensuring its proper placement Ref Fig 6 Fig 6 10 Retention Spoke Plate Sample Holder www mpbio com Locking Pin Hole Under the Tube for matching Al...

Страница 10: ...ove each sample tube cap Place the securing knob ratchet nut on this assembly to tighten it completely Ref Fig 7 Fig 7 8 Close the dome properly and secure the dome clip WARNING Sample tubes must be secured properly before running the FastPrep 24 Instrument 11 Sample Tube Retention Spoke Plate www mpbio com ...

Страница 11: ... setting can be saved as described in next section can be changed at this moment Press p or q key to change the default Speed to User Selectable Speed Once speed is selected press SET again flashing of 4 0M S will stop to indicate that speed has been selected Switch ON Power Supply using Rocker Switch Provided at the Back Make sure Emergency Switch is pulled out On Power ON the LCD will Show WELCO...

Страница 12: ... to indicate the Run time can be changed using p or q keys Once Run time is selected press SET key again Flashing of LCD will stop to indicate that Run time has been selected Once speed tube holder and time have been selected or saved Press RUN key LCD will show SURE press RUN key to start the session If you are ready to start then press the RUN Key again The motor will start and display will show...

Страница 13: ...now use p key to reach up to the message Program1 press SET key display will show P1 S 4 0 and value 4 0 will be flashing which indicates that the speed set in Program 1 is 4 0 M S use p or q key to change Speed Value Once Speed has been Set press SET key to save in program 1 and now LCD will show P1TH MP Letter MP will be Flashing use p or q key to Set the desired tube holder Once tube holder has...

Страница 14: ...inutes between runs 4 The Instrument should be properly grounded for safe use Earth terminal Temperature Indication In case LCD displays SYS WARM it is an indication that Critical Parts of the system are getting Hot User should provide a PAUSE before next RUN In case LCD displays SYS HOT it is an indication that temperature of critical parts is High Unit will stop automatically and user will not b...

Страница 15: ...nts Speed Range 4 0 6 5m sec Programmable in 0 5 m sec increments Acceleration 2 seconds to maximum speed Deceleration 2 seconds to stop Weight 17 9 Kg Power Requirement 110VAC 60Hz or 220 VAC 50 Hz 400 W Operating Temperature 15ºC to 35ºC Storage Temperature 5ºC to 43ºC Dimensions LXWXH 33 2 cm X 43 7 cm X 46 5 cm 16 www mpbio com ...

Страница 16: ...d springs rubber cups dome tube holders tube covers motor and its attachments other accessories WARRANTY DISCLAIMERS The warranty is in lieu of all warranties expressed or implied and no representative or person is authorized to assume for manufacturer any other liable in connection with the sale of our products There shall be no claims for defects or failure of performance or product failure any ...

Страница 17: ...harge is made for service and return postage Please return the product insured packed with sufficient protection postage insurance prepaid to the address Any duty brokerage fee if any must be paid by the consumer OUT OF WARRANTY SERVICE There will be charges rendered for repairs made to the product after the expiration of the aforesaid one 1 year warranty period after purchaser is advised appropri...

Страница 18: ...gs are provided If a spring breaks follow the steps below Ref Fig 8 1 Open the dome Securing knob ratchet from the shaft 2 Remove the sample holder all plastic pieces 3 Put the screw driver through the hole provided on the face of aluminium collared bush holding unscrew the bottom spring holder top end of spring s screw 4 Remove damaged spring replace it with the new spring It is recommended that ...

Страница 19: ...f Fig 9 1 Ensure that input power is disconnected during replacement of fuse 2 Take out fuse receptacle from the AC receptacle provided at the back side of main instrument A spare fuse is provided in fuse holder Remove the faulty fuse and replace with new one 3 Now insert the fuse receptacle into the AC receptacle carefully 4 Connect the power cord to the instrument plug it into a compatible outle...

Страница 20: ... in the FastPrep 24 Instrument spills should be cleaned immediately and appropriate decontamination carried out The FastPrep 24 Instrument may not be resistant to all cleaning regimens required for all infections agents Exercise appropriate caution and wear protective clothing eyewear and gloves when working with potentially infectious samples Contaminated units should be kept in an appropriate bi...

Страница 21: ... second refer to the maximum vertical velocity achieved by a sample tube during reciprocating motion The rate of collision is proportional to speed while the energy of impact is proportional to the square of the speed For example a 50 increase in the FastPrep 24 Instrument speed setting increased the rate of collision by 50 and at the same time increases the energy of impact by 125 The FastPrep 24...

Страница 22: ...particles the lysing matrix FastPrep 24 Kits that contain Lysing Matrix Tubes and purification reagents A specific Lysing Matrix for each application 23 Lysing Matrix Sample Extraction A B C D E F G H I J K Animal bacteria yeast fungi plant Bacteria spores Yeast fungi Animal and plant Soil sediments water feces All kind of samples optimization DNA RNA proteins RNA proteins RNA proteins DNA RNA All...

Страница 23: ... one 1 4 inch ceramic sphere Extra 1 4 inch ceramic spheres are packaged separately Lysing Matrix A tubes have orange caps and found in the FastDNA and FastDNA SPIN Kits Lysing Matrix B Each impact resistant 2 0 ml tube contains 0 1 mm silica spheres Lysing Matrix B tubes have blue caps and are found in the FastRNA Pro Blue Kit for isolation of total RNA from gram positive and gram negative bacter...

Страница 24: ...ml tube contains white granules and silicon carbide Lysing Matrix F tubes have white caps Lysing Matrix G Each impact resistant 2 0 ml tube contains silicon carbide and 2 mm glass beads Lysing Matrix G tubes have brown caps Lysing Matrix H Each impact resistant 2 0 ml tube contains 2 0 ml glass beads and 2 mm yellow zirconia beads Lysing Matrix H tubes have yellow caps Lysing Matrix I Each impact ...

Страница 25: ... have brown caps and are found in the GENECLEAN for Ancient DNA Kit Addidtional Lysing Matrices One the rare occasion that matrices A E do not homogenize totally the sample the researcher may wish to consider the texture of the sample and test lysing matrix F K tubes Smooth and or muscular tissue may need a rough particle like garnet to tear it Hard brittle samples may need a 6 mm ceramic sphere 2...

Страница 26: ...High quantity 3 Reproducibility 1 Purity 2 Reproducibility 3 High quantity 1 Purity 2 Reproducibility 3 High quantity 1 Purity 2 Reproducibility 3 High quantity 1 Purity 2 Reproducibility 3 High quantity FastDNA Kit FastDNA SPIN Kit FastDNA SPIN Kit for Soil FastRNA Pro Blue Kit FastRNA Pro Red Kit FastRNA Pro Green Kit FastRNA Pro Soil Direct and Indirect Kits TM FastProtein Blue Matrix TM FastPr...

Страница 27: ...ne cartilage or seeds Homogenization in the FastPrep 24 instrument with Lysing Matrix A takes place in the presence of sample specific Cell Lysis Solutions CLS For plant tissues CLS VF is used in conjunction with a Protein Precipitation Solution PPS Yeast algae and fungi are lysed in the presence of CLS Y For all other samples CLS TC is used during sample lysis For maximum flexibility all buffers ...

Страница 28: ...ly difficult sources such as eubacterial spores and endospores gram positive bacteria yeast algae nematodes and fungi Homogenization in the Fastprep 24 instrument with Lysing Matrix E takes place in the presence of MT Buffer and Sodium Phosphate Buffer reagents carefully developed to protect and solubilize nucleic acids and proteins upon cell lysis These reagents work together to allow extraction ...

Страница 29: ...tion and contain RNA stabilizers Then the researcher just finishes with chloroform extraction and precipitation TM TM FastPROTEIN Blue and FastPROTEIN Red Matrix TM TM FastPROTEIN Blue Matrix and FastPROTEIN Red Matrix are designed for bacteria and yeast protein extraction respectively These matrices are ideal for optimizing induction conditions and quickly lyse samples from a range of conditions ...

Страница 30: ...tPrep 24 SMPS 48V Display PCB Driver PCB Warning This Part Must be Replaced by Qualified Technician or MP Biomedicals Technical Service 6004503A 6004503B 6003502 6003504 A 6002505 Motor Bowl EA EA EA EA EA Cat No Description Package Size www mpbio com ...

Страница 31: ...e Replaced by Qualified Technician or MP Biomedicals Technical Service 6002506 6002507 6002508 6002509 6002510 Polycarbonate Dome with cap and Stopper Dome Rubber Gasket Dome Clip Lock pins for Dome Locking Rachet nut pack of 2 EA EA pack of 2 EA www mpbio com ...

Страница 32: ...e Replaced by Qualified Technician or MP Biomedicals Technical Service 6002511 6002512 6002513 6002514 6002515 6002516 Rachet Bolt Sample Holder Set 3 STEP Assembly with studded bearings Spring assembly set with hooks Rubber Shoe EA EA EA EA pack of 4 pack of 4 www mpbio com ...

Страница 33: ...n AC Cord 3Pin flat Ac Cord for USA UL AC Receptacle with fuse Rocker Switch UL Emergency Switch EA EA EA EA EA Warning This Part Must be Replaced by Qualified Technician or MP Biomedicals Technical Service Cat No Description Package Size www mpbio com ...

Страница 34: ...IMPORTANT As the design and manufacture of our product is subject to constant improvement the product supplied may differ in some details from the specification and ...

Страница 35: ...www mpbio com MP Biomedicals 29525 Fountain Parkway Solon OH 44139 tel 1 800 854 0530 fax 1 800 334 6999 Instruction Manual Instruction Manual Ver 4 0 Worldwide Ordering and Technical Support ...

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