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Stepped Microtip

9-5

Extender Tip

9-6

Sapphire Tipped Horn

9-6, 9-7

Sonabox

9-7

Dual Horn

9-7

Tray Horn

9-7

Sonabox Diagram

9-8

10.

Troubleshooting

10-1

Overload Indicator Instructions

10-1

Troubleshooting Guide

10-1

11.

Maintenance

11-1

General Maintenance

11-1

Cleaning Instructions

11-1

Tip Care

11-2

12.

Applications

12-1

Principle of Ultrasonics

12-1

Processing Tissue

12-1

Processing Difficult Cells

12-2

13.

Techniques

13-1

Probe Depth

13-1

Foaming and Aerosoling

13-1

Viscosity Limitations

13-1

Keeping Samples Cool

13-1

Free Radical Formation

13-1

14.

Warranty

14-1

15.

Return of Equipment

15-1

16.

Literature Request

16-1

Bibliographies

16-1

Application Notes

16-1

Published Papers

16-2

Technical Notes

16-2

Technical Bulletins

16-2

Содержание SONICATOR XL2010

Страница 1: ...CESSOR OPERATION MANUAL M I S O N I X MISONIX INCORPORATED MODELS XL2020 XL2015 XL2010 MISONIX INCORPORATED 1938 New Highway Farmingdale NY 11735 U S A Telephone 631 694 9555 Fax 631 694 9412 Toll Free 1 800 645 9846 www misonix com ...

Страница 2: ... XL2015 Functions 5 3 XL2010 XL2015 Controls and Indicators Diagram 5 4 6 Preparation for Use 6 1 Convertor Mounting Diagram 6 2 Removal and Attachment of Horns and Tips 6 3 Removal and Attachment of Horns and Tips Diagram 6 4 7 Tuning Instructions 7 1 XL2020 Tuning Instructions Probes Horns 7 1 XL2010 XL2015 Tuning Instructions Probes Horns 7 2 MicrotipTM and Cup Horn Instructions 7 3 8 Programmi...

Страница 3: ...ing Instructions 11 1 Tip Care 11 2 12 Applications 12 1 Principle of Ultrasonics 12 1 Processing Tissue 12 1 Processing Difficult Cells 12 2 13 Techniques 13 1 Probe Depth 13 1 Foaming and Aerosoling 13 1 Viscosity Limitations 13 1 Keeping Samples Cool 13 1 Free Radical Formation 13 1 14 Warranty 14 1 15 Return of Equipment 15 1 16 Literature Request 16 1 Bibliographies 16 1 Application Notes 16 ...

Страница 4: ...ble High voltage is present in the cable and may pose a shock hazard The generator must be properly grounded with a 3 prong plug Test electrical outlet for proper grounding before plugging in unit Install the SONICATOR in an area free from excessive dust dirt explosive or corrosive fumes and protected from extremes in temperature and humidity NEVER immerse the convertor in liquids of any kind or l...

Страница 5: ...ate DO NOT OPERATE DAMAGED EQUIPMENT Retain all packing materials for possible future shipments POWER REQUIREMENTS For power requirements check the label on the back of the unit 1 For 110V operation the generator requires a single phase three wire 110V 50 60 Hz line supply unless otherwise specified 2 For 220V operation the generator requires a single phase three wire 220V 50 60 Hz line supply unl...

Страница 6: ... x 2 5 Dia 20 3cm x 6 4cm 8 L x 2 5 Dia 20 3cm x 6 4cm Materials Aluminum Aluminum Standard Horn Weight 0 5 lbs 0 45Kg 0 5 lbs 0 45Kg Dimensions 5 L x1 5 Dia 12 7cm x 3 8cm 5 L x1 5 Dia 12 7cm x 3 8cm Materials Titanium Alloy Titanium Alloy Environmental Pollution Degree Two 2 Two 2 Installation Category II II Temperature Limits 50 F to 110 F 10 C to 43 C 50 F to 110 F 10 C to 43 C Barometric Pres...

Страница 7: ...s intermittent operation permits high intensity sonication while avoiding heat build up in the processed sample It also allows particulate material being processed to settle back under the tip OVERLOAD INDICATOR under certain extraordinary conditions the amount of power supplied to the convertor horn assembly might endanger the circuitry of the generator to the point of failure In order to prevent...

Страница 8: ...age of acoustic amplification It includes a removable or solid tip a 20 stud for attachment to the convertor and external threads around the body for attachment of accessories e g Flow Cells The Horn is available in three standard tip diameters of or 1 In addition each horn comes in three different tip configurations Tapped horns have a replaceable Flat Tip Solid horns do not have a replaceable ti...

Страница 9: ...listed below and prompts 10 Elapsed Time Display Displays actual elapsed time of sonication 11 PULSARTM OFF Time Indicates pulse OFF time from 0 1 seconds to 1 hour 12 Overload Indicator Indicates a load condition greater than the maximum output power of the generator or that the unit requires tuning or other readjustments 13 PULSARTM Indicator Indicates application of pulsed energy 14 PAUSE Key I...

Страница 10: ...maximum available ultrasonic power delivered to the horn i e 40 output 0 4 x 550 Watts available 220 Watts delivered 5 PULSARTM Cycle Timer XL2015 Only Activates pulsed energy Pulse width adjusts from 0 05 of a second 5 duty to 0 95 of a second 95 duty every second 6 Amplitude Control Knob Controls the amplitude of ultrasonic vibration at the tip of the horn 7 Tuning Control Knob Allows adjustment...

Страница 11: ... check the tightness of the horn and flat tip by using the wrenches provided and referring to 6 3 6 4 of this manual An insufficiently tightened horn or tip will inhibit tuning and might cause damage to the generator circuitry or mating parts of the convertor and horn NEVER assemble or disassemble the probe by holding the convertor in a vise ALWAYS use the wrenches supplied with the unit 8 Mount t...

Страница 12: ... of the holes at the base of the horn 2 Place the opening of the 7 16 open end wrench on the wrench flats of the Flat Tip MICROTIP or Extender 3 Apply even and firm pressure CAUTION An insufficiently tightened horn probe or tip will inhibit tuning and might cause damage to the generator circuitry or mating parts of the convertor and horn NEVER assemble or disassemble the probe by holding the conve...

Страница 13: ...trol Knob see 5 2 fully counter clockwise to zero 2 Press Power Switch to the ON position The switch should be illuminated 3 Wait until the prompt For Tuning Procedure Refer to Manual appears on the LCD display then press the TUNE key and the LCD will display as follows Tuning Probe Active 4 Turn the Amplitude Control Knob slowly towards setting 3 4 1 Pay strong attention to the bar graph of the L...

Страница 14: ...d TRAY HORNS 1 Turn Amplitude Control Knob see 5 4 fully counter clockwise to zero and turn Pulsar Duty cycle to off for the Model XL2015 2 Press Power Switch to the ON position The switch should be illuminated 3 Press and hold down the TUNE switch 4 Turn the Amplitude Control Knob slowly towards setting 3 4 1 Pay strong attention to the needle of the analog power meter STOP turning the Amplitude ...

Страница 15: ...L2020 or the needle of the analog power meter on the XL2010 XL2015 STOP turning the Amplitude Control Knob if the reading exceeds 70 3 2 Rotate the Tuning Knob see 5 2 or 5 4 either clockwise or counter clockwise until the lowest possible reading usually below 20 is achieved 4 Turn the Amplitude Control Knob slowly towards setting 3 and repeat steps 3 1 and 3 2 5 Turn the Amplitude Control Knob sl...

Страница 16: ... Key Action TUNE Activates or Deactivates tuning circuit START STOP Starts or Stops output in all modes of operation Resets timer in timed mode PROG DATA Initiates Timer and PULSARTM entry program and displays entries on the LCD screen during run ENTER Enters data during program mode CLEAR Clears previous or erroneous entries Exits programming mode PAUSE Interrupts or Resumes processing cycle 60 7...

Страница 17: ...then switches to For Tuning Procedure Refer to Manual alternates with If SONICATOR is Tuned Press CLEAR Clear introduction message Press CLEAR Key Press PROG to Program Timer and PULSAR or alternates with Press START for Continuous Operation Start sonication in continuous mode Turn Amplitude Control Knob to desired level Press START Key STOP Continuous 0 00 00 Elapsed Time begins to count seconds ...

Страница 18: ...sec Then Press ENTER Enter total processing time from 00 00 01 to 9 59 59 For example 20 minutes Press 2 0 0 0 Keys and then press ENTER Key Enter Pulse ON Time 00 00 0 min sec tenths alternates with Then Press ENTER 00 00 0 min sec tenths Skip pulsing feature Enter 0 for pulse ON time Press 0 Key and then press ENTER Key 0 20 00 PROG START CLEAR 00 00 0 MEM 0 00 00 0 Start sonication in timed mod...

Страница 19: ...ter Total Process Time alternates with 0 00 00 hr min sec Then Press ENTER Skip timer feature Enter 0 for total process time Press 0 Key and then press ENTER Key Enter Pulse ON Time 00 00 0 min sec tenths alternates with Then Press ENTER 00 00 0 min sec tenths Enter pulse ON time from 00 00 00 1 to 59 59 00 1 For example 30 seconds Press 3 0 0 Keys and then press ENTER Key Enter Pulse OFF Time min...

Страница 20: ...0 0 Select the default memory location MEM 0 Press PROG Key DATA 0 00 00 hr min sec Enter Total Process Time alternates with 0 00 00 hr min sec Then Press ENTER Enter total processing time from 00 00 01 to 9 59 59 For example 10 minutes Press 1 0 0 0 Keys and then press ENTER Key Enter Pulse ON Time 00 00 0 min sec tenths alternates with Then Press ENTER 00 00 0 min sec tenths Enter pulse ON time ...

Страница 21: ...ower Switch SONICATOR XL2020 MISONIX INCORPORATED Scrolls on screen then switches to For Tuning Procedure Refer to Manual alternates with If SONICATOR is Tuned Press CLEAR Clear introduction message Press CLEAR Key Press PROG to Program Timer and PULSAR or alternates with Press START for Continuous Operation Begin programming Press PROG Key DATA 0 00 00 PROG START CLEAR 00 00 0 MEM 0 00 00 0 Choos...

Страница 22: ...pters to inlet outlet and overflow ports 6 Tune the system before adding water or other coolant follow the tuning instructions outlined in 7 1 through 7 3 7 When filling the cup horn allow room for displacement when sample is added For constant flow cooling use hose adapters with soft tubing Provide pinch clamps on the Inlet and outlet tubes to control flow An open bath recirculating chiller is re...

Страница 23: ...ges or centers Conical tubes should be tilted to expose maximum sample area to the horn Samples should be wide and shallow rather than high and narrow Maintain a small clearance around the vessel to allow adequate coolant flow Suspend vessels at least to 3 to 6 mm above the face of the horn Energy in the cup can be determined easily by the pattern of sonication cavitation bubbles or micro streamin...

Страница 24: ...tional effect The cell includes two orifice plates drilled 1 8 and 3 32 Flow through the cell can be controlled by the orifice size alone or by suitable outlet valving The metal parts orifice plates holder and retainer are stainless steel and the O rings are Buna N nitrile synthetic rubber Transparent Polycarbonate Model Part 800A This model permits observation of the sonication process The materi...

Страница 25: ... retainer and holder are stainless steel and have very fine threads shipped with a light film of machine oil to prevent galling If the oil film is removed with the use of solvents or any other means apply molybdenum disulfide powdered Teflon graphite or other appropriate lubricant Stainless Steel Model Part 800C 1 Clean cell and all metallic parts with appropriate solution 2 Install 228 O ring on ...

Страница 26: ...g at the desired power setting below setting 5 on the Amplitude Control Knob the MICROTIP limit 3 Always immerse the MICROTIP in liquid before turning on the instrument Immersing a live MICROTIP may cause splashing aerosoling or foaming 4 If the material being treated aerosols or foams the generator should be turned off immediately as this is equivalent to operating the MICROTIP in air 5 The MICRO...

Страница 27: ...an not be used on removable flat tips because the wrenching forces are transmitted to the disc and cause fracture Sapphire discs are not applied to horns under 12 7 mm in radiating face diameter 2 Sapphire tip discs are very hard with a rating of 9 0 on the Mohs scale where diamond is 10 However they are also easily fractured by mechanical shock or bending Therefore avoid any contact between the t...

Страница 28: ...ews Pass coolant tubing through holes Invert Enclosure If the Acoustic Enclosure is to be used exclusively with the cup horn the latch handle may be reversed to close downward Remove inner nut which secures pawl DO NOT allow adjusting outer nut to rotate Remove pawl rotate it 180 on shaft reinstall pawl and nut and tighten nut DUAL HORN The Dual Horn accessory allows a single SONICATOR to process ...

Страница 29: ...dicator light OVERLOAD REFER TO MANUAL When overload occurs for all models proceed with steps 1 7 below 1 Shut the unit off using main power switch 2 Check all electrical connections 3 Eliminate contact between horn and sample For flow cell applications reduce static pressure if necessary 4 Check tightness of horns and or tips Remove tips and clean threads before tightening 5 Check horn for cracks...

Страница 30: ...movement possible and no cracks or frayed ends visible Do not subject the cable ends to severe bending loads while performing these tests Order a replacement cable immediately if your cable does not pass the above inspection Should the convertor cable assembly be subjected to misuse such as dropping or a severe pulling force on the wire itself the cable must be inspected as above DO NOT USE A CABL...

Страница 31: ...ill release tiny glass and titanium particles into the sample which may gray the solution The particles will form a thin dark gray layer on centrifuging but usually have an insignificant effect chemically Proper care of the probe is essential for dependable service The intense cavitation will after a prolonged period cause the tip to erode and decrease the efficiency of the sonication process with...

Страница 32: ...ocedure in 7 1 through 7 3 The SONICATOR Ultrasonic Liquid Processor will break down cells bacteria spores or tissue It can prepare an emulsion down to 1 100 of a micron homogenize immiscible liquids polymerize some materials de polymerize others and accelerate chemical reactions By virtue of this instrument faster and more efficient extraction of serums toxins enzymes and viruses from organic sou...

Страница 33: ...staphylococcus collagenase can be used with skin and cartilage and trypsin hyaluronidase can be used with liver and kidney Glass beads in the 10 to 50 micron size range can also be added to the liquid to expedite processing When using glass beads a good ratio is beads liquid Glass beads concentrate the energy released by the implosion and facilitate the crushing of cell walls After processing the ...

Страница 34: ...um to reduce a tenacious foam If foam persists the sample may have to be discarded VISCOSITY LIMITATIONS Viscous solutions and highly concentrated liquids can be difficult to sonicate If the liquid is so thick that it will not pour or circulate easily or if 25 micron glass powder will not settle to the bottom quickly it is too thick and cannot be processed effectively KEEPING SAMPLES COOL Intense ...

Страница 35: ...years from the date of original delivery The obligation of MISONIX Incorporated under this guaranty will be to make any repair at its plant in Farmingdale New York or at any of its designated service centers without charge including parts and labor but not including shipping charges necessitated by any defect in workmanship or material This guarantee is in lieu of any warranty of any kind or natur...

Страница 36: ...r and generator separately in plastic bags film or other protective wrapping when using loose packing materials such as foam pellets shredded paper or excelsior Remove MICROTIP probes or extenders and pack separately in same carton IMPORTANT NOTICE BY RETURNING ANY MATERIAL TO MISONIX INC THE CUSTOMER OR THE CUSTOMER S AGENT THUS CERTIFIES THAT ANY AND ALL MATERIALS SO RETURNED ARE OR HAVE BEEN RE...

Страница 37: ...ication of Ultrasonics in Geology and Earth Sciences BIB 9 Ultrasound and Disinfection BIB 10 Ultrasonics and Liposomes BIB 11 Sonochemistry Ultrasonic Cavitation and Chemical Reactions BIB 12 Suslick Bibliography on Sonochemisty BIB 13 Biological Research Update BIB 14 Ceramics and Soils Research Update BIB 15 Industrial Applications Update BIB 16 Food Technology Update APPLICATION NOTES AN 1 Bre...

Страница 38: ...easurement TN 2 Extenders and Sapphire Tips TN 3 Extending Control Range TN 4 Microtips TN 5 Half Wave Length Extender Tips TN 6 Flow Cells TN 7 Development of Protocols TECHNICAL BULLETINS TB 1 Ultrasound Hearing and Health TB 3 Sifting Solids TB 4 Pulsed Sonication TB 5 ASTM Test Method G 32 Mail Request To MISONIX INCORPORATED Literature Request 1938 New Highway Farmingdale NY 11735 Ship Litera...

Страница 39: ..._____________________ 5 4 XL2010 XL2015 CONTROLS AND INDICATORS DIAGRAM Control Index 1 Overload Indicator 2 Tuning Switch 3 Power Switch 4 Power Output Display 5 PULSARTM Cycle Timer XL2015 Only 6 Amplitude Control Knob 7 Tuning Control Knob 8 SHV Cable Connector 9 Footswitch Jack 10 Power Receptacle 11 Fuse 10 amp 115V 8 amp 220V OVERLOAD PULSAR DUTY CYCLE 1sec TUNE TUNE ...

Страница 40: ...STOP Key 2 PULSARTM On Time 10 Elapsed Time Display 18 Amplitude Control Knob 3 Power Output Display 11 PULSARTM Off Time 19 Tuning Control Knob 4 Pause Indicator 12 Overload Indicator 20 SHV Cable Connector 5 Timer Indicator 13 PULSARTM Indicator 21 Footswitch Jack 6 CLEAR Key 14 PAUSE Key 22 Power Receptacle 7 ENTER Key 15 TUNE Key 23 Fuse 10 amp 115V 8 Power Switch 16 PROG DATA Key 8 amp 220V 4...

Страница 41: ...Manual_________________________________________________________Preparation for Use _________________________________________________________________________________________________ 6 2 CONVERTOR MOUNTING DIAGRAM ...

Страница 42: ..._______Accessories _________________________________________________________________________________________________ 9 8 SONABOX DIAGRAM S U P E R S O N A B O X A S S E M B L Y F O R S T A N D A R D H O R N S S U P E R S O N A B O X A S S E M B L Y F O R C U P H O R N U S E ...

Страница 43: ..._______________________________________________________________________________________________ 2 2 SYSTEM COMPONENTS DIAGRAM 1 Operating Manual 2 High Frequency Cable 3 7 16 X Open End Wrench 4 Generator 5 Convertor 6 Power Cord 7 2 Pin Spanner Wrenches 8 Removable Flat Tip 9 Tip Diameter Horn ...

Страница 44: ... Use _________________________________________________________________________________________________ 6 4 REMOVAL AND ATTACHMENT OF HORNS AND TIPS DIAGRAM Microtip Removal Microtip Attachment 1 7 16 X Open End 2 Pin Spanner Wrenches Horn Removal Horn Attachment Tip Removal Tip Attachment ...

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