MAKING A STANDARD % GLUCOSE SOLUTION
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5. Using plastic pipettes, rinse prism and sample well with
distilled or deionized water. Wipe dry. The instrument is ready
for the next sample.
8. MAKING A STANDARD % GLUCOSE SOLUTION
To make a Glucose Solution, follow the procedure below:
y
Place container (such as a glass vial or dropper bottle that has
a cover) on an analytical balance.
y
Tare the balance.
y
To make an X Glucose solution weigh out X grams of high
purity Glucose (CAS #: 50-99-7) directly into the container.
y
Add distilled or deionized water to the container so the total
weight of the solution is 100g.
Note:
Solutions above 20% may need to be vigorously stirred
or shaken and heated in a water bath to roughly 40 °C (104 °F).
Remove solution when glucose has dissolved. Cool completely
before use. The total quantity can be scaled proportionally for
smaller containers but accuracy may be sacrificed.
Example with 10% Glucose:
%
g
Glucose
g
Water
Total
25
10.000
90.000
100.000
