manualshive.com logo in svg

Millipore Amicon Ultra-2, Руководство пользователя, Страница: 8 / 11

Поделиться
Скачать
Millipore Amicon Ultra-2 Скачать руководство пользователя страница 8

Amicon

®

 Ultra-2 Centrifugal Filter Devices

8

Specifications

Maximum initial sample volume

 

2.0 mL

Typical final concentrate volume 

30–70 µL depending on MWCO

Maximum relative centrifugal force

Swinging bucket rotor

4,000 × g for concentration spin, 

 

1,000 × g for recovery spin

Fixed angle rotor

7,500 × g for concentration spin, 

 

1,000 × g for recovery spin

NOTE:  

When spinning viscous solutions such as 

 

undiluted serum or plasma, do not exceed 5,400 x g.

Active membrane area

1 cm

2

Hold-up volume

< 5 μL

Dimensions

Filter device and tube

Length (concentration mode; device in filtrate tube):                        119.7 mm (4.71 in.)
Length (recovery spin; device upside down in concentrate tube):      95.3 mm (3.75 in.)

Filter device

Diameter:  15.9 mm (0.63 in.)

Length:     70.7 mm (2.78 in.)

Filtrate tube

Diameter:  13.8 mm (0.54 in.)

Length:     52.9 mm (2.08 in.)

Concentrate tube

Diameter:  13.7 mm (0.54 in.)

Length:     34.5 mm (1.36 in.)

Materials of Construction

Filter device

Copolymer styrene/butadiene

Membrane

Ultracel

®

 low-binding regenerated cellulose

Collection tubes

Polypropylene

Содержание Amicon Ultra-2

Страница 1: ...pun in a swinging bucket or fixed angle rotor Amicon Ultra 2 devices are supplied non sterile and are for single use only The Amicon Ultra 2 product line includes 5 different cutoffs Molecular Weight...

Страница 2: ...Failure to fully seat the device in the filtrate collection tube and push the concentrate collection tube firmly onto the device may result in the device breaking during centrifugation See figure bel...

Страница 3: ...mL protein in 100 mM NaCl 50 L of 40 mg mL protein in 100 mM NaCl 100 mM NaCl 50 L of 40 mg mL protein in 12 3 mM NaCl 12 3 mM NaCl Add 1 95 mL of 10 mM NaCl or exchange buffer 2 mL of 1 mg mL protei...

Страница 4: ...10 to 20 minutes of centrifugation the lowest concentrate volume 30 70 L is reached after spinning for 10 to 60 minutes Figure 1 Typical Filtrate Volume vs Spin Time for Amicon Ultra 2 Device Swingin...

Страница 5: ...nd 10K BSA for 30K and 50K and IgG for 100K n 8 Shaded volumes were used for the calculation of protein recovery in Table 5 Table 3 Typical Concentrate Volume Concentration Factor v Spin Time Fixed An...

Страница 6: ...nging bucket rotor 4 000 g or 35 fixed angle rotor 7 500 g 2 mL starting volume room temperature n 12 Factors that determine sample recovery include the nature of the protein solute relative to the de...

Страница 7: ...te collection tube and concentrate collection tube before use 2 Fill filter device with solution and reweigh 3 Assemble device in filtrate collection tube and centrifuge per instructions 4 Collect the...

Страница 8: ...rum or plasma do not exceed 5 400 x g Active membrane area 1 cm2 Hold up volume 5 L Dimensions Filter device and tube Length concentration mode device in filtrate tube 119 7 mm 4 71 in Length recovery...

Страница 9: ...ent 0 1 Tergazyme detergent 1 Lubrol PX detergent 0 1 Triton X 100 surfactant 0 1 Nonidet P 40 surfactant 2 Tween 20 surfactant 0 1 Sodium deoxycholate 5 Organic solvents Acetone Not recommended Ethyl...

Страница 10: ...FC900396 10K 8 24 96 500 UFC501008 UFC501024 UFC501096 UFC5010BK UFC201024 UFC801008 UFC801024 UFC801096 UFC901008 UFC901024 UFC901096 10K IVD 8 24 96 UFC801008D UFC801024D UFC801096D UFC901008D UFC90...

Страница 11: ...office nearest you go to www sigmaaldrich com offices Technical Assistance Visit the tech service page on our web site at www sigmaaldrich com techservice Standard Warranty The applicable warranty fo...

Отзывы:

Нет отзывов