00110230w Rev 10/20
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Message
General Cause
Corrective Action
Warning High concentration
Concentration of sample
is too high
Dilute sample so that it is no higher than recommended maximum
operating range guideline. See
Lost Start
Sample volume too small,
sensor not fully immersed
in solution while sample is
loading, or air bubble in sensor
Make sure sample volume is ≥ 100 µL. Keep sensor fully
immersed while screen displays. Keep the Sensor Submerged.
False start
Sensor malfunction
Replace with unused sensor.
False stop
Start/Stop short
Previously used sensor
Sensors are not reuseable, replace with unused sensor.
Electrode short
Sensor malfunction
Replace sensor.
Aperture block
Sample population size
exceeds aperture size
Dilute to reduce incidences, but may not eliminate. Use
D-PBS for diluting solution. See
Viscous sample
(e.g. serum-enriched media)
Keep sensor submerged in sample until Count Complete appears
on the display.
Use D-PBS for diluting sample.
Cell Counter sensor is blocked
Sample concentration is too high; dilute cell sample more.
Ensure that the cells are in a single-cell suspension. Break
clumps by pipetting up and down with a standard pipettor or
pre-filter using Steriflip
®
filter unit with 20 µm nylon net.
Air bubble in sensor due to:
Pre-mature pressing of OK
button
Fully submerge sensor in sample before pressing OK button.
Air bubble in sample
Mix sample gently to avoid air bubbles.
Sensor lifted from sample
before screen displays
Sample
Loaded
Keep sensor submerged in sample while screen displays Keep
the Sensor Submerged.
Wrong diluting solution
Refer to list of acceptable diluting solutions in
.
Debris from diluting solution
Diluents can be filtered to remove debris using ≥0.2 µm using
Stericup
®
, Steritop
®
or Steriflip
®
filters. See
for catalogue numbers.
Open Start
Issues detected upon sensor
insertion
Re-insert sensor. Try new sensor, if problem persists, contact
Tech Service.
Open stop
Previously used sensor
detected
Re-insert sensor. Try new sensor, if problem persists, contact
Tech Service.
System stopped
Error occurred or Menu
bottom pressed during
counting to abort test
Replace sensor and retest.
No histograms
currently stored
No stored data
The instrument is new or all histograms have been deleted.
Unsuccessful file transfer
Some histograms
failed to export
Low battery
or USB thumb drive
not inserted
or USB thumb drive
not fully inserted
or USB thumb drive is
malfunctioning or full
or USB thumb drive is
not formatted to FAT32
Charge battery and re-try file transfer.
Push USB thumb drive into the Scepter™ 3.0
USB port firmly.
Check the USB thumb drive to ensure it is
formatted FAT32, has sufficient space available
and is functioning properly.
See
Can not mount
USB thumb drive
