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2   PDQeX Instruction Manual 

201

90513

 

Purpose of the PDQeX 

MicroGEM's 

PDQeX 

Nucleic  Acid  Extractor  extracts

  DNA  from  a  wide  range  of 

biological  substances.  It  should  only  be  used  by  trained  personnel  or  under 
supervision. 
The PDQeX is designed to extract DNA from the following types of samples. If your 
target  material  is  not  covered  in  this  document,  please  contact  us 

at 

[email protected]

.  

Forensic evidence or Human Identification (HID) samples containing:

o

Saliva

o

Blood

o

Hair follicles

o

Spermatozoa

o

Human or animal cell tissue

o

Touch / trace samples

Plant material

o

Leaves and stems

o

Plant pathogens

Animal tissue

o

Muscle and Fat

o

Hair follicles

Bacteria

DNA Viruses

Insects

The  PDQeX  family  of 

devices

  is  intended  to  assist  scientists  and  technicians  to 

simplify  their  workflow  by  reducing  DNA  extraction  to  hands-off,  automated 
processes. This is achieved inside a disposable, sealed cartridge and thereby protects 
the integrity of the sample and isolates staff from potential pathogens. 

The PDQeX uses different cartridges for different substrates. Each cartridge is designed 
to  perform  a  single  extraction  and  the  reagents  within  the  cartridges  are  formulated 
differently for different substrates (tissue, saliva, semen, blood, plant). 

The elegance and simplicity of this system removes the need for robotic extraction or 
time-consuming manipulation by a laboratory technician. 

WARNING:  Do  not  modify  this  equipment  without  authorisation 

from the manufacturer. 

Содержание forensicGEM

Страница 1: ...PDQeX Nucleic Acid Extractor Instructions for use Applicable models forensicGEM phytoGEM prepGEM IMPORTANT Read the instructions before operating this device...

Страница 2: ...and Fat o Hair follicles Bacteria DNA Viruses Insects The PDQeX family of devices is intended to assist scientists and technicians to simplify their workflow by reducing DNA extraction to hands off au...

Страница 3: ...hydrolases and a program on the PDQeX to activate them 2 A temperature regimen is carried out by the hardware This brings into play the different enzymes at different temperatures The final temperatu...

Страница 4: ...access for disconnection from the main supply Back of the PDQeX Only use the power supply provided with the PDQeX Do not place the PDQeX close to any RF transmitter A low power transmitter such as a c...

Страница 5: ...easier for you to remove the spent cartridges after the run has finished Pull out the collection tube rack see the picture on the previous page if you are unsure where this is and place 0 2 ml PCR tub...

Страница 6: ...wer the hinged flap onto the caps of the cartridges and close the sliding door MAKE SURE THE CARTRIDGE POSITIONS IN THE BLOCK CORRESPOND TO THE COLLECTION TUBES BELOW OTHERWISE YOU WILL CONTAMINATE TH...

Страница 7: ...of them You will then be able to slide out the drawer Cap your tubes and store at 20 C Notes The door of the machine will lock while the block is hot If you wish to carry out sequential runs make sure...

Страница 8: ...n To the left of the screen is a map to help you navigate the program 2 System Settings The System Settings window allows you to 1 Set Date and Time 2 Reset to Factory Defaults 3 Provide links so that...

Страница 9: ...Instruction Manual 20190513 2 2 Date and Time Set the Date and Time using the keypad and press Save The last System option gives you a QR code so that you can access more information from the MicroGEM...

Страница 10: ...is above the heating block During UV treatment the sliding door will be locked Press UV Treatment on the Home screen The procedure takes 30 minutes and can be interrupted at any time You can press In...

Страница 11: ...rogram you wish to run The temperature regimen will be shown Press Start The PDQeX will switch to a display that shows the progress of your program At any time you can terminate the program but be awa...

Страница 12: ...specific sample type Press the Button Click on the dashed line at the top of the screen to give your program a name You cannot save your program without a name Press on the temperature time place hold...

Страница 13: ...sensor at the back of the collection tube drawer If the drawer is not in place or is crooked the machine will not run This prevents the DNA from being seriously contaminated The block is hot and we d...

Страница 14: ...pened swabs and wipes Aluminium parts of this machine should not be soaked in Virkon or Bleach Extended use of chemical agents such as these will corrode the parts WARNING DO NOT GET LIQUIDS INSIDE TH...

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