Section 3: Running an ITC Experiment
3.7 Using the ThermoVac for Cleaning the ITC
Sample Cell
The ThermoVac may be used to flush copious amounts of detergent solution followed by a
thorough rinse with distilled water.
•
Insert the Cell Cleaning Apparatus into the Adapter, until the top flange is touching the top of
the Adapter (see below).
•
Insert the long needle into the Sample cell and push down carefully until the o’ring has
sealed, as shown below.
•
The end of the plastic tubing from the upper tube of the Cell cleaning Apparatus is immersed
into a beaker of 200-400 ml of detergent cleaning solution.
•
The end of the plastic tubing from lower tube is connected to a one liter vacuum flask
through the #8 rubber stopper.
•
The side arm of the vacuum flask is attached to the Vacuum Port of the ThermoVac. Your
Setup up should look like below.
47
