4
www.bioneer.com
BQ-042-101-05
Revision : 2 (2016-07-04)
IX.
Product Specifications
Sample Type
Mini
(Typical yield)
Midi
(Typical yield)
Maxi
(Typical yield)
Whole blood
200 μl ( < 10 μg)
2 ml ( < 80 μg)
4 ml ( < 150 μg)
Cultured cell
~ 1 x 10
6
( < 12 μg)
~ 5 x 10
6
(< 60 μg)
~ 1 x 10
7
(< 120 μg)
Animal tissue
~ 25 mg ( < 10 μg)
~ 100 mg ( < 40 μg)
~ 250 mg (< 120 μg)
Bacteria
(Gram (-), (+))
~ 1 x 10
9
(< 15 μg)
~ 5 x 10
9
(< 80 μg)
~ 1 x 10
10
(< 150 μg)
Expected purity
A
260
/
280
> 1.8, A
260
/
230
> 1.4
* The yield of low copy cell could be less than the figures shown in the table.
** For cultured cells, lower cell numbers can be used to a minimal cell number of ~10
4
for “micro” scale
extraction.
Small amount of sample
MagListo
TM
5M Genomic DNA Extraction Kit is also able to extract genomic DNA from a small quantity of
sample. If the sample is low copy cell (< 1x10
4
) or has a small amount of DNA, we recommend that about
4 μg of carrier DNA (a homopolymer such as poly-dA, poly-dT, or gDNA) or RNA should be added to the
starting material. Ensure that the carrier DNA does not interfere with your downstream application. Carrier
RNA can be removed later by RNase digestion. Refer to “DNA Extraction from Cultured Cell for Micro”
which is optimized protocol for extraction of DNA from low copy cell in page 10
X.
Principle
MagListo
TM
5M Genomic DNA Extraction Kit is designed for the extraction of genomic DNA from a variety
of sources including high molecular weight up to 40 Kb (Note: this is often for most DNA based
application). The overall principle is based on adsorption of DNA onto the magnetic nano bead by
chaotropic salt. For instance, chaotropic agents in Buffer ② (Binding) contains guanidine hydrochloride,
as which removes water molecules around DNA and silica coated magnetic bead surface resulting in
genomic DNA then being captured by magnetic beads. The magnetic nano bead and nucleic acid
complexes are pulled and fixed on the tube wall using a magnetic force, followed by washing with ethanol
