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4.2 Sample Preparation
4.2.1 Materials required
Cell suspension
Luna™ Cell Counting Slides
Trypan Blue Stain 0.4 %
Luna
™ USB drive or equivalent
4.2.2 Prepare cell suspension according to standard cell biology procedure. Please avoid clumping
of cells for accurate counting.
4.2.3 Mix 10
l of the cell suspension with 10
l of Trypan Blue Stain in an appropriate tube by
gentle pipetting up and down.
4.3 Loading Samples into the Luna
™ Cell Counting Slide
Hold the edge of the slide and load 10
‒ 12
l of the mixed cell sample into the sample loading port
of one chamber of the counting slide. Alternatively, the slide can be placed on a clean surface
during sample loading.
For easy and accurate loading, tilt the pipette 45
‒ 60 degrees as shown below.
Note
: Be careful not to over-load or under-load the sample into the chamber.
