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User Manual

www.logosbio.com

LBSM-MD-ML-LUY-001

VL1712-01

Содержание Luna-II yf

Страница 1: ...User Manual www logosbio com LBSM MD ML LUY 001 VL1712 01...

Страница 2: ...d can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a res...

Страница 3: ...r 3 Protocol Settings 13 3 1 Protocol Parameters 13 3 2 Creating and Editing Protocols 14 3 3 Protocol Selection 15 Chapter 4 Counting Yeast Cells 16 4 1 Instrument Preparation 16 4 2 Sample Preparati...

Страница 4: ...erly connected If the instrument is not grounded the electrical safety of the product cannot be guaranteed 8 Turn the instrument on only after connecting the power cord and AC adapter to both the powe...

Страница 5: ...inutes of mixing samples for accurate cell viability measurements If necessary count your sample twice duplicate readings and take an average 3 As the LUNA II YF is calibrated before shipping recalibr...

Страница 6: ...F also provides the option to review previous data that can be exported to USB as a CSV file Both reusable and disposable slides can be used with the LUNA II YF Designed for cost efficient an accurate...

Страница 7: ...sing option at the specified concentration of Saccharomyces cerevisiae Component Quantity LUNA II YF Automated Yeast Cell Counter 1 Power Cord with AC Adapter 1 PhotonSlide 50 Slides 1 box Acridine Or...

Страница 8: ...hscreen a power button a slide port to insert slides and a USB port for easy data transfer 1 5 2 Rear View of the LUNA II YF The rear of the LUNA II YF has two USB ports and a power inlet to connect t...

Страница 9: ...Do not install the instrument in a location that will expose the instrument to intense ultraviolet light 2 2 Startup Main Menu Push the power button below the touchscreen to turn the instrument on The...

Страница 10: ...the most recent calibration and the date and version of the latest software update Settings options 2 3 1 Save Options Preset the types of files saved and customize the file name prefix 2 3 2 Dilutio...

Страница 11: ...f live and dead cells Raw Image Untagged images of cells in each channel Report PDF report with count data and histograms Select the desired saving options by pressing the boxes in front of each optio...

Страница 12: ...o use for subsequent dilution calculations Press Dilution Calculator Options in the Settings screen Select the desired value The selection will be marked with a yellow circle Press to return to the Se...

Страница 13: ...t remove the slide or turn off the instrument during this process The background calibration value and date will have changed in the Settings Screen 2 3 5 Settings Software Update Logos Biosystems con...

Страница 14: ...ctively see Section 4 2 Sample Preparation Assuming a final sample volume of 20 L users can modify this value according to the following table Yeast Volume 20 L 18 L 17 L 16 L 15 L 14 L 12 L 10 L 8 L...

Страница 15: ...be modified or deleted To create a new protocol select one of the default protocols and press Save as Using the onscreen keyboard rename the protocol and press Save The newly created protocol will ap...

Страница 16: ...tate and Propidium Iodide select the FDA PI protocol Select the desired protocol in the Protocol screen Press Load to apply the selected protocol Now the instrument is ready to count cells with the se...

Страница 17: ...in Pipette gently Incubate the sample for 10 minutes at room temperature FDA PI Mix 17 L yeast suspension with 1 L Fluorescence Signal Enhancer 1 1 L Fluorescein Diacetate Stain and 1 L Propidium Iodi...

Страница 18: ...l appear to the right of the screen The GF button indicates that the green fluorescence channel is on Press GF to change it to RF and switch to the red fluorescence channel To view the stained cells u...

Страница 19: ...the image The red outer box in the top left corner of the image represents the entire counting area and the inner box is the current field of view The size and location of the inner box will change w...

Страница 20: ...channel and a merged image of both channels Press Overlay to change it to GF and display live cells Press GF to change it to RF and display dead cells Press RF to change it to Overlay and display a me...

Страница 21: ...y the distribution of only live cells Press Live on to change it to Dead on and display the distribution of only dead cells The LUNA II YF provides a gating function that can be controlled by the gati...

Страница 22: ...nt concentration options are Total Live Dead and Custom Users may set the concentration to be used for the dilution calculation by pressing the grey box below the Current Concentration value to be the...

Страница 23: ...tings see Section 2 3 1 Settings Save Options Using the onscreen keyboard name the count as desired A default file name prefix may be set in the Settings 2 3 1 Settings Save Options Users may add the...

Страница 24: ...s Avg size indicates the fluorescent object size Cell Count Report File name AOPI_07032016_161208 Date 07 MAR 2016 16 08 Cell count results Total 7 09 x 10e5 cells mL Live 6 60 x 10e5 cells mL Dead 4...

Страница 25: ...es the fluorescent object size If available in the folder thumbnails of captured images will appear below the results Tap on the thumbnails to make it full size Users can view the raw images captured...

Страница 26: ...sh the power button for five seconds 6 2 Cleaning Turn the LUNA II YF off and disconnect the power cable before cleaning Make sure that liquids do not enter any part of the instrument during cleaning...

Страница 27: ...otocol or create a new protocol Make sure the appropriate dilution factor has been selected Poorly aligned images Wait for cells to settle in the slide chambers prior to counting Data transfer and sav...

Страница 28: ...x 0 5 mL F23002 Acridine Orange Stain 2 x 0 5 mL F23003 Propidium Iodide Stain 2 x 0 5 mL F23004 Propidium Iodide Stain for Yeast 2 x 0 5 mL F23211 Fluorescein Diacetate Stain 2 x 0 5 mL F23213 Fluore...

Страница 29: ...strument is in original conditions or 2 after 30 calendar days of purchase only replace or repair the Instrument for up to the Warranty Period without issuing a credit In no event shall the Company ac...

Страница 30: ...31 478 4184 Email sales logosbio com USA 7700 Little River Turnpike STE 207 Annandale VA 22003 USA Tel 1 703 622 4660 Tel 1 703 942 8867 Fax 1 517 266 3925 Email sales logosbio com EUROPE 11B avenue...

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