15
Select the
STED
mode.
Set the WLL laser line that you need to use ( for ex.
488nm
at
30 % to 50% transmission
).
Select the Hyd 2 detector and set it at
100%.
Introduce
to the Hyd a bandwidth that is
10nm fahter
to the WLL
line that you set, and maximum
10nm lower
than the
wavelength of the laser depletion ( +/-582nm).
Open the
Gate Setting
; set the
Wll
value which is equal to
the value you use to excite the fluorochrome (for ex
488nm
)
Set the
Gate Start
from 0,5 to 2,5
1,5
is compromise
Set the
Gate End
to
6,5
STED configuration ( step2)
Содержание Sp5 WLL STED
Страница 3: ...3 System Start up step 1 On the STED Tower Switch ON the button...
Страница 7: ...7 Turn on the XCite Light System Start up step 5...
Страница 18: ...18 System Shut Down step 1 Before you exit from the software PLEASE clean the objective Turn off the 3 lasers...
Страница 19: ...19 System Shut Down step 2 Exit from the LASAF software Go to Start and shut down the computer...
Страница 20: ...20 Turn on the XCite Light System Shut Down step 3...
Страница 22: ...22 System Shut Down step 5 On the STED Tower turn the Key vertical position Switch off the button...
