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From Eye to Insight

Personal AUtomated Lab Assistant

Instructions for use

Leica Microsystems CMS GmbH

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Instructions for use 11934164, Revision 1.0, 2018-06-15

PAULA

Содержание PAULA

Страница 1: ...From Eye to Insight Personal AUtomated Lab Assistant Instructions for use Leica Microsystems CMS GmbH Instructions for use 11934164 Revision 1 0 2018 06 15 PAULA...

Страница 2: ...ithout express written permission from Leica Microsystems CMS GmbH The instructions contained in the following documentation reflect state of the art technology We have com piled the texts and illustr...

Страница 3: ...6 2 Local ad hoc pairing network 15 7 Supported control devices 16 8 Getting started 17 8 1 Scope of Delivery 17 8 2 Unpacking 18 8 3 Placement of the Digital Imager 18 8 4 Connectivity set up 19 8 5...

Страница 4: ...or or moder ate injury Caution In this manual additional safety notes are indicated with the triangle symbol shown here and have a gray background Caution The instrument and accessories can be damaged...

Страница 5: ...ning of hazardous laser radiation take appro priate safety precautions Instructions for disposing of the instrument its ac cessories and consumables Explanatory note Item not contained in all equipmen...

Страница 6: ...hting purposes or room lighting Reasonably foreseeable misuse The following are prohibited Using PAULA system for any purpose not in accordance with the Declaration of Conformity e g using it as an in...

Страница 7: ...ers Leica Microsystems CMS GmbH offers special microscope variants with additional safety features Laser couplings require corresponding safety devices that have to be inspect ed and installed by trai...

Страница 8: ...follow the instructions and warnings contained in this user manual The instruments and accessories described in this manual have been safety tested and checked for possible hazards The responsible Lei...

Страница 9: ...ing Power NUC6i7KYK via an external power supply Only use the LPS certified Power Supply Delta Electronics ADP 120RH D General specifications NUC6i7KYK PC Supply voltage 19V DC Power consumption max 9...

Страница 10: ...ding 2000 m ASL NL Transport and storage in a range of 10 70 C and at a humidity not exceeding 80 non condensing Make sure that a free air circulation WARNING By definition the main circuit breaker of...

Страница 11: ...efore cleaning Pro tect electrical components from moisture Do not spray Isopropanol not 70 ethanol on the surfaces and cables Do not dip the imager nor the NUC PC nor any of the cables in Isopropanol...

Страница 12: ...de reader Embedded in the front of the digital imager Core software Control of digital imager including image acquisition t lapse and confluence check browse and search function Optional apps Transfec...

Страница 13: ...4 System Overview Fig 2 Dimensions of the digital imager in mm Fig 3 Top View PAULA 4 2 Intel NUC PC Please refer to the instruction for use delivered with the Intel NUC PC 13...

Страница 14: ...Intel NUC PC Ensure that the Digital Imager is used only by persons authorized to do so Ensure that the Digital Imager user manual is always available Check on a regular basis that the users are comp...

Страница 15: ...uch monitor no local area network LAN needed or you can operate PAULA using tablets Windows Android or iOS tablets are supported For this purpose PAULA NUC PC must be integrated into your local networ...

Страница 16: ...in the same network as the Intel NUC PC Use the set up service of your tablet to connect the client Windows Android or iOS tablet to the desired network Ensure that the Intel NUC PC is connected to t...

Страница 17: ...ve Windows 10 Pro Processor Intel Core i7 6650u CPU 2 20GHz 2 21GHz Screen size inch 9 7 9 7 10 6 Resolution pixel 1536 x 2048 1536 x 2048 1366 x 768 Storage RAM GB 16 16 16 Tested and recommended tab...

Страница 18: ...bols indicating environment friendly recycling Before you start the set up of PAULA please remove the transport lock at the rear side of the digital imager 8 3 Placement of the Digital Imager The Digi...

Страница 19: ...USB3 cable from the Digital Imager with the appropriate interface at the rear side of the NUC PC any socket Connect the power supply of the Digital Imager to the mains Plug in the power supply cord a...

Страница 20: ...s well This might limit the simultaneous use of addi tional digital imagers Please follow instructions for use of your touch monitor Switch on NUC PC and the touch monitor An icon of PAULA App is disp...

Страница 21: ...you want to do Connect PAULA to a LAN 8 4 2 1 Set up local network with a travel router 6 2 8 4 2 1 Add the Digital Imager to a local area network LAN Note Make sure that the LAN meets the minimum req...

Страница 22: ...ocal network a permanent static IP address is provided In this case you can start the PAULA app on your tablet directly 8 6 If the NUC PC does not have a permanent IP address please click on the Leica...

Страница 23: ...assays 1 2 3 4 5 Switch between Acquisition and Data View Use the Acquisition View to define your imaging parameters and start cell based assays Use the Data View to monitor and organize your results...

Страница 24: ...rs Switch between live still image image acquisition Switch between channels Phase contrast red and green fluorescence For each channel a slider is displayed which allows to define the intensity Overl...

Страница 25: ...dependent if they are assigned to a project or a particular flask You can select if you want to see in addition all images acquired during a t lapse recording and or if you want to show the results of...

Страница 26: ...are prompted to select your PAULA device Please compare the last 3 digits with the last digits of the serial number of your PAULA The serial number is displayed at the rear side of the cell checker I...

Страница 27: ...mination settings Barcode reader 9 1 User management rules Only one user per time can log in No other user except administrator can log in in parallel If the logged in user does not touch the system f...

Страница 28: ...o User Management Go to Users Create new user assign administrator rights and delete current user Note If you want to benefit from the notification function you need to provide your e mail address and...

Страница 29: ...men Insert name of the cell line You can use a predefined cell line from the pull down menu or you can create your own name Optional insert plasmid name Optional insert passage Optional insert comment...

Страница 30: ...ck has been performed during the acquisition of the t lapse the sum mary is in Data View as well What do you want to do next Enable notification service 9 2 Activate Confluence Check or another cell b...

Страница 31: ...he flask of interest and select t lapse of interest The summary page of the t lapse is displayed In case a cell based assay has been performed dur ing acquisition of the t lapse you can select the re...

Страница 32: ...ransfection Efficiency Measurement of percentage of fluorescent cells Not yet available Cell Counting Count of cells Not yet available Wound healing Coverage of a specified scratched area Not yet avai...

Страница 33: ...8 5 1 What do you want to do next Check the image and the outline in the Data View 8 5 2 Start a t lapse in combination with the confluence check 10 3 2 Perform a confluence check on up to 10 images...

Страница 34: ...pse or the confluence control panel Check the results in Data View under flask Double click on the t lapse to see the results You can switch between the graph view or a list view to see the individual...

Страница 35: ...All available apps for cell based assays will be displayed Currently only Confluence Check available Select assay of interest and insert licence number which will be delivered separately by e mail an...

Страница 36: ...ent and enable notify me and define the exact time point in the control panel for confluence check I cannot activate the barcode reader Open specimen definition panel and activate the barcode icon If...

Страница 37: ...cted computing devices which share one data communication system net Physically near 8 same building Eth ernet and WiFi are the most used connections between the members of a LAN Static IP address The...

Страница 38: ...ntrated soap solution petroleum ether or ethyl alcohol For cleaning coated parts use a linen or leather cloth that is moistened with one of these substances Caution Thinners containing acetone xylene...

Страница 39: ...equipment type Paula is in compliance with Directive 2014 53 EU The full text of the EU declaration of conformity is available at the following internet ad dress http www leica microsystems com produ...

Страница 40: ...Leica logos are registered trademarks of Leica IR GmbH Order nos of the editions in English German French 11934164 Printed on chlorine free bleached paper Revision 1 0 issued 2018 05 15 Leica Microsy...

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