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Leica DVM6 User Manual 

  

2

Contents

General Instructions

General Instructions 

5

Important Safety Notes 

6

Symbols Used 

7

Safety Instructions 

8

Introduction

Congratulations! 11
Packing and Unpacking 

12

Standard Delivery and Accessories 

13

System Overview 

15

Overview of the Instrument 

16

Instrument Components 

16

Leica DVM6 C 

17

Leica DVM6 S 

17

Leica DVM6 A 

18

Leica DVM6 M 

18

Objectives 19

Installation/Assembly

Setting up Leica DVM6 

21

Installing the PlanAPO Objective 

22

Cables and Terminals 

23

Optional Accessories  

24

Polarizer 24

Diffuser 25
Side Light Adapter 

26

Transmitted Light Stage 

27

Leica LED5000 SLI External Illumination 

28

Personalizing the Computer and Installing Software 

29

Instrument Setup

Leica DVM6 

32

Switching on the Leica DVM6 

32

Switching on the Computer and Starting the LAS X Software 

33

Placing the Sample and Positioning the Stage 

34

Focusing 35
Changing the Magnification 

37

Using a Tilting Stand 

38

Application

Leica DVM6 Application Example 

41

Leica DVM6 

41

Changing the Objective 

43

Coaxial Illumination 

44

Using Accessories 

47

Polarizer, Diffuser 

47

Side Light Adapter, Transmitted Light Stage  

48

Leica LED5000 SLI External Illumination  

49

Содержание DVM6

Страница 1: ...Leica DVM6 User Manual...

Страница 2: ...inals 23 Optional Accessories 24 Polarizer 24 Diffuser 25 Side Light Adapter 26 Transmitted Light Stage 27 Leica LED5000 SLI External Illumination 28 Personalizing the Computer and Installing Software...

Страница 3: ...ca LAS X Basic Functions 51 Service Care Maintenance Contact Persons 54 Spare parts 57 Accessories 58 Specifications Specifications 60 Dimensional Drawings 69 Objectives 69 Leica DVM6 C S A 70 Leica D...

Страница 4: ...Leica DVM6 User Manual General Instructions 4 General Instructions...

Страница 5: ...Do not use any unsuitable cleaning agents chemicals or techniques for cleaning O O Never use chemicals to clean colored surfaces or accessories with rubberized parts This could damage the surfaces an...

Страница 6: ...anuals and updates are also available for you to download and print from our website www leica microsystems com This User Manual describes the special func tions of the Leica DVM6 digital microscope s...

Страница 7: ...nstruments Warning of hazardous electrical voltage This symbol indicates especially impor tant information that is mandatory to read and observe Failure to comply can cause the following O O Hazards t...

Страница 8: ...that are outside of the scope of this manual Unauthorized alterations to the instrument or non compliant use shall void all rights to any warranty claims Place of use O O Refer to Safety Concept book...

Страница 9: ...Health risks Workplaces with microscopes facilitate and improve the viewing task but they also impose high demands on the eyes and holding muscles of the user Depending on the duration of unin terrupt...

Страница 10: ...Leica DVM6 User Manual Introduction 10 Introduction...

Страница 11: ...LAS X is required for displaying and editing the images from the Leica DVM6 and for controlling the Leica DVM6 and selected accessories This means you will be perfectly equipped for any situation inc...

Страница 12: ...micro scope weighs approximately 20 kg Keep the original packaging Always use the original packaging to transport the Leica DVM6 LEICA DVM6 LEICA DVM6 1 2 To avoid damaging the Leica DVM6 never hold i...

Страница 13: ...r supplying power 4 USB cable for connection to the computer 5 Leica Application Suite X LAS X software DVD Required accessories 6 Country specific power cable 7 Objective 8 Windows computer with Wind...

Страница 14: ...adapter 4 Transmitted light stage 5 Dust cover 6 Flexible Leica LED5000 SLI point light source For information on additional accessories such as objectives and adapters illumination or the dust cover...

Страница 15: ...view 1 2 3 4 5 LEICA DVM6 ThisoverviewshowstheLeicaDVM6inanexam ple application with additional components 1 Zoom module 2 Tilting stand with rocker arm 3 PlanAPO FOV 12 55 objective 4 XY stage with s...

Страница 16: ...t objectives are available for selection 8 Contrast dial for coaxial incident light illumination for PlanAPO FOV 12 55 and FOV 3 60 objectives 9 LED ring light illumination coaxial illumination 10 XY...

Страница 17: ...controlled exclusively via the Leica LAS X software LEICA DVM6 On the Leica DVM6 S 10 450 702 the focus drive is encoded and can be operated both motorized and manually the XY stage is encoded and ca...

Страница 18: ...the motorized focus drive The exact position of the sample can be controlled on the screen in the precise detail LEICA DVM6 The Leica DVM6 M consists of the zoom module and can only be used with a spe...

Страница 19: ...a PlanAPO FOV 12 55 10 450 705 objective is the objective for medium magnifi cation levels It has a working distance of 33 mm Using the zoom ring you can select the visible sample section ranging from...

Страница 20: ...Leica DVM6 User Manual Installation Assembly 20 Installation Assembly...

Страница 21: ...hat the Leica DVM6 is far enough away from the screen to ensure the tilt ing movement of the microscope The Leica DVM6 microscope is designed as a VDU workstation the microscope image is always and on...

Страница 22: ...rical parts Installing the objective The objective weighs approximately 2 kg Therefore hold it firmly during assembly and disassembly Remove any samples from the XY stage before assembly 1 Take the ob...

Страница 23: ...hat the Leica DVM6 is switched off 2 Connect the Leica DVM6 to a suitable power socket using a power supply and power cable 3 Connect the Windows PC to the power supply 4 Using the USB cable connect t...

Страница 24: ...ossible damage to the samples remove them from the XY stage Rotate the focus drive downwards to create more room for assembly 1 Attach the polarizer so that it perfectly fits onto the objective 2 Lock...

Страница 25: ...ling accessories Assembly To prevent possible damage to the samples remove them from the XY stage Rotate the focus drive downwards to create more room for assembly 1 Attach the diffuser so that it per...

Страница 26: ...the distance to the sample Assembly Remove the samples from the XY stage to prevent possible damage Rotate the focus drive downwards to create more room for assembly 1 Attach the side light adapter so...

Страница 27: ...ns mitted light stage Assembly Remove any samples from the XY stage Rotate the focus drive downwards to create more room for assembly 1 Remove the stage plate from the XY stage The stage plate is secu...

Страница 28: ...ase contact your Leica Microsystems representative Power supply via Leica DVM6 1 Make sure that the Leica DVM6 is switched off 2 Connect the provided cable to the Leica LED5000 SLI The right and left...

Страница 29: ...For instructions on installing and using the software refer to the DVD provided with the Leica DVM6 Computer provided by the customer The appropriate version of Leica Appli cation Suite X LAS X must...

Страница 30: ...with a 16 9 or 16 10 aspect ratio is recommended for displaying the image using LAS X This leaves a sufficient amount of space on the monitor next to the live display for the LAS X software control e...

Страница 31: ...Leica DVM6 User Manual Instrument Setup 31 Instrument Setup...

Страница 32: ...pe and is securely in place 1 For the PlanAPO FOV 12 55 and FOV 3 60 objectives Rotate the relief contrast controller all the way forward open and set the contrast dial to the normal position left sto...

Страница 33: ...ion in an unmounted state Ensure that the objective is inserted on the microscope and is securely in place the microscope is switched on and the micro scope is connected to the computer via USB Use 1...

Страница 34: ...ter of the stage plate 2 Now you can move the stage manually or using the LAS X software until the desired section is displayed in the live image On a Leica DVM6 with motorized XY stage double clickin...

Страница 35: ...r lowers the XY stage using the focusing drive Focusing is manual or motor ized depending on the instrument version The sample detail is brought into sharp focus as soon as it is at the focal point 1...

Страница 36: ...e 4 Working distance for FOV 12 55 planapo chromatic objective The working distance for the PlanAPO FOV 12 55 objective is shown in the image You can see the working distance for other objectives on p...

Страница 37: ...ve image 4 Refocus if necessary The Leica DVM6 provides you with infi nitely variable adjustment for changing the magnification level Parfocality The Leica DVM6 is parfocally matched If the sample is...

Страница 38: ...e tilting stand with the zoom module and objective in a range from 0 to 60 The maximum tilting angle depends on the objective being used and the size and height of the sample The Leica DVM6 compensate...

Страница 39: ...before the objec tive collides with the XY stage while the micro scope is being tilted O O The warning tone triggers based on the assumption that you are using a flat sample Exercise caution when usin...

Страница 40: ...Leica DVM6 User Manual Application 40 Application...

Страница 41: ...n levels without special preparation O O The Leica Application Suite X LAS X soft ware lets you control the Leica DVM6 change settings and record images or videos Following you will find a brief descr...

Страница 42: ...as it will go 7 Position the sample on the stage plate You may have to move and rotate the sample 8 Focus using the coarse and fine adjustment 9 Now increase the magnification level and refocus if ne...

Страница 43: ...les from the XY stage before changing the objective Make sure not to touch optical and elec trical parts The objective is changed during ongoing operations hot swap You do not need to switch off the L...

Страница 44: ...and FOV 3 60 objectives Switching on the coaxial illumination 1 Focus on the sample 2 Switch on the coaxial illumination using the Leica LAS X software 3 You can also regulate the brightness of the co...

Страница 45: ...wait a moment until the exposure correction has compensated for the brightness of the image The contrast dial should only be turned out of the normal position for flat shin ing samples to improve the...

Страница 46: ...patial impression 1 With the coaxial illumination switched on bring the sample into focus 2 Use the control dial to adjust the coaxial illumination until the image shows the desired spatial effect Adj...

Страница 47: ...ation direction of the LED ring light illumination O O This can display low contrast samples with high contrast Potential material errors or other visible defects can be identified quickly and reliabl...

Страница 48: ...at a shallow angle This accentuates irregularities on the sample surface e g waviness or scratches Transmitted light stage O O When using the transmitted light stage 10 450 712 the light is directed t...

Страница 49: ...ontrolled using the Leica LAS X software O O The intensity of the illumination can be adjusted in 10 increments O O It can also be operated using the separate gooseneck with integrated control unit Co...

Страница 50: ...Leica DVM6 User Manual Software Application 50 Software Application...

Страница 51: ...ences are not listed in this User Manual Image area O O The current image is shown in the image area The displayed status indicator on the top right indicates whether a live image a paused image or a...

Страница 52: ...e the display Information on the image currently shown Show a scale in the image Status bar The current instrument status is shown in the status bar For more information a detailed descrip tion of the...

Страница 53: ...Leica DVM6 User Manual Service 53 Service...

Страница 54: ...did on the very first day Warranty benefits The guarantee covers all faults in materials and manufacture It does not however cover damage resulting from careless or improper handling Contact address I...

Страница 55: ...ssive use of solvents The lint free cloth lens cloth or cotton swab should be soaked with solvent but not so wet that solvent runs over the lens O O Protect your microscope from moisture fumes and aci...

Страница 56: ...using distilled water O O Remove dust with a bellows and a soft paintbrush O O Clean the objectives with special optics cleaning cloths and a commercially avail able glass cleaner Servicing O O Repair...

Страница 57: ...M6 User Manual Service 57 Spare parts 1 2 3 4 Item Leica article number Designation 1 19 002 518 Power supply 2 19 005 838 USB3 0 cable 2 m 3 19 005 829 Stage plate 4 10 725 566 Fastening screw for ob...

Страница 58: ...pter for DVM6 objective FOV 43 75 FOV 12 55 objectives 5 10 450 709 Diffuser adapter for objective FOV 43 75 provides dome like illumination when used with FOV 12 55 objective 6 10 450 717 Diffuser ad...

Страница 59: ...Leica DVM6 User Manual Specifications 59 Specifications...

Страница 60: ...Motorized Tilting stand LAS X software Combinable objectives PlanAPO FOV 43 75 10 450 704 o o o PlanAPO FOV 12 55 10 450 705 o o o PlanAPO FOV 3 60 10 450 706 o o o Transmitted light option 10 450 71...

Страница 61: ...3 664 2 748 pixels Pixel size 1 67 1 67 m Exposure time 0 5 to 500 ms Frame rate live 1 600 1 200 pixels Maximum 37 frames per second Image acquisition resolution 2 megapixels 1 600 1 200 pixels 5 me...

Страница 62: ...OV 43 75 PlanAPO FOV 12 55 PlanAPO FOV 3 60 Design engineering Main objective multi layered tempered lead free Correction Planapochromatic Connection Quick change system with slide in mechanism Magnif...

Страница 63: ...58 394 to 789 V min 4 to 9 15 to 30 53 to 105 Object field 3 wSys V max 2 19 mm 0 63 mm 0 18 mm V min 35 0 mm 10 04 mm 2 88 mm hSys V max 1 64 mm 0 47 mm 0 14 mm V min 26 3 mm 7 53 mm 2 16 mm Depth of...

Страница 64: ...lp mm 22 lp mm 77 lp mm Useful magnification range 1 2 V max 17 to 34 42 to 84 96 to 193 V min 1 or 2 4 to 7 13 to 26 Object field 3 wSys V max 2 19 mm 0 63 mm 0 18 mm V min 35 0 mm 10 04 mm 2 88 mm h...

Страница 65: ...D LED Coaxial incident light Integrated Integrated Light source LED LED Contrast dial 4 plate rotatable Relief contrast Ring light illumination accessories Diffuser Adapter o o Polarizer Adapter o o D...

Страница 66: ...using range Z direction 60 mm Drive Manual coarse fine Motorized Resolution 0 5 m 0 25 m 0 25 m Stand DVM6 C DVM6 S DVM6 A Tilting stand Tilting angle 60 to 60 Zero position vertically Indexed Locking...

Страница 67: ...ear side of tilting stand CTL2 for Leica LED 5000SLI Rear side of tilting stand Power supply Input 100 V AC to 240 V AC 3 2 A Output 33 V DC 3 93 A max Electrical interfaces DVM6 C DVM6 S DVM6 A Tempe...

Страница 68: ...LAS X software modules LAS X Core LAS X Live Image Builder XY LAS X Live Image Builder Z LAS X 2D Measurements LAS X 2D Annotations LAS X Image EDF including 3D SurfaceViewer LAS X 3D surface measure...

Страница 69: ...Leica DVM6 User Manual Specifications 69 Dimensional Drawings Objectives Leica PlanAPO FOV 3 60 Leica PlanAPO FOV 12 55 Leica PlanAPO FOV 43 75 84 84 84 74 60 144 144 144 102 33 5 130 Dimensions in mm...

Страница 70: ...Leica DVM6 User Manual Specifications 70 Leica DVM6 C S A 134 758 60 60 260 435 647 260 435 420 473 442 326 max 230 518 647 LEICA DVM6 Dimensions in mm...

Страница 71: ...Leica DVM6 User Manual Specifications 71 Leica DVM6 M 154 376 228 LEICA DVM6 Dimensions in mm...

Страница 72: ...Leica DVM6 User Manual Terms Used 72 Terms Used...

Страница 73: ...d and the numerical aperture Diffuser The diffuser illuminates the samples from a large range of angles This illuminates reflec tive samples in a more uniform manner and prevents glares in the image F...

Страница 74: ...n birefringence selective absorption or scattering Polarization can be used to differentiate changes in the structure and the composition of the material that cannot be detected with ordinary light Re...

Страница 75: ...tance on an image on a monitor to the corresponding distance on the object O O Visual display magnification This corre sponds to the lateral display magnifica tion when viewing a digital image on a mo...

Страница 76: ...Leica DVM6 User Manual Troubleshooting 76 Troubleshooting...

Страница 77: ...the zoom ring to the lowest magnification level When using a coaxial illumination set the relief contrast to the OPEN setting The motorized focus drive cannot be activated in the LAS X software Ensure...

Страница 78: ...ves up when a Leica DVM6 with a motorized focus drive is restarted Remove samples and other objects from the XY stage to avoid damaging the objective in use Error Correction Uneven image brightness wh...

Страница 79: ...to modifications LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH CONNECT WITH US www leica microsystems com Leica Microsystems Schweiz AG Max Schmidheiny Strasse 201...

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