Updated 06.09.2019
Nélia Castro
Thin-section microscope – Leica DMPL with Leica
camera MC170HD
User instructions
1.
Switch on camera power (and switch OFF when you are finished!). Select “PC” state
on the back of the camera of things do not work (NOT “HDMI” state, which is for the
HDMI connector to a television).
First time users
, read “To ease calibration” textbox
at the end of this page
2.
Optional: Start IrfanView (thumbs) (or ACDSee) (for parallel use, to browse images,
renaming etc.) and go to relevant computer folder (your M:-
disk). Normally files are saved at “M:\My Documents\My
Pictures\” or “M:\pc\Pictures\”.
3.
Start LAS (Leica Application Suite) (see icon
right)
4.
Switch on micr light, find relevant thin section and
magnification (carry out specific microscope setting, like
cross-polar, transmitted and/or reflected light). Do necessary
light adjustments.
“Captured format” should be 2592 x 1944 pixls (=5
megapixel image)
5.
Important: “Calibrate” must be ticked !
a.
Optional: Left-mouse drag a square on a white/grey part
on the thin section (our outside the actual thin section),
and select “White balance”. White balance (colour cast)
will then be kept neutral until someone changes the
lamp power (requires then a new white balance
configuration).
b.
Click “Acquire”
c.
Select correct magnification for the objective in use
(2,5x, 5x a.s.o.).
6.
In “Process” – Scale bar tick “Show” (move scale bar) and
click “Merge”
7.
Do required adjustments. Change lightning if necessary (the images should be a bit darker than what you
actually like because then it is quite easy to edit them in e.g. PhotoShop; at least make sure that no white
part is totally burnt out).
End of session: If needed copy your images to your USB stick.
Switch off camera power.
