Leica DM L Instructions Manual Download

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Leica DM L

Instructions

Summary of Contents for DM L

Page 1: ...Leica DM L Instructions...

Page 2: ...tion issued in 1998 by Leica Microsystems Wetzlar GmbH Ernst Leitz Strasse D 35578 Wetzlar P O Box 20 40 Responsible for contents Microscopy product management Phone 49 0 64 41 29 25 19 Fax 49 0 64 41...

Page 3: ...survey observation 33 Operation 34 Basic setting for transmitted light 34 Filters 36 Condensers Koehler illumination 37 Phase contrast 42 Transmitted light darkfield 44 Transmitted light polarization...

Page 4: ...DMLB 100 W lamp with switched mode power supply 100 S 020 519 507 to 020 519 511 Leica DMLP and DMLM 100 Wlamp with switched mode power supply 100 S 020 520 701 to 020 520 718 Distribution voltage mai...

Page 5: ...C prisms compensator 12 Tube lens system in the tube 13 Tube prisms 14 Eyepiece intermediate image 14 field diaphragm not part of all configurations Incident light path 16 20 Vertical illuminator 15 L...

Page 6: ...wn It applies to the microscope Leica DM LB mainly for biological and medical applications and together with a supple mentary manual to the microscopes DM LP polarized light and DM LM material examina...

Page 7: ...r area or the main factory in Wetzlar Attention Attention Attention Unpacking documents Please compare the delivery carefully with the packing note delivery note or invoice We strongly recommend that...

Page 8: ...he objectives and condenser 14 7 Remove the stage after loosening the clamp screw 28 13 Block the vertical movement of the stage bracket by fitting hard foam rubber between the microscope base stage b...

Page 9: ...of lamphousings lamp change p 21 23 Gas discharge lamps Hg Xe p 24 are always fed by external power units For incident light the 100 W halogen lamp can alternatively also be run on an external power...

Page 10: ...33 7 put filter magazine back in position 4 1 33 7 Filter holder Attachment 2 filters or Pol components with 32 mm diameter 32 5 can be inserted in the filter holder 5 3 32 1 The slit above the ICT P...

Page 11: ...table on p 32 If the condenser is not yet complete the following components may have to be fitted before the condenser is adapted to the microscope Fig 14 see p 16 for fitting details Auxiliary conde...

Page 12: ...itting the UCL condenser disc 1 Condenser disc 2 Light ring for darkfield or phase contrast or whole quarter wave compensator 3 Centering screws 4 Axis 5 Centering keys 6 or 4 compensator 7 Auxiliary...

Page 13: ...h a 6 position disc 10 5 and Fig 11 The largest position should be used for brightfield and therefore left empty if possible To disassemble the disc Unscrew the fastening screw of the disc 11 9 which...

Page 14: ...condenser make sure that neither of the centering screws 11 8 is sticking out at the side ICT condenser prisms Insert the ICT condenser prisms K2 K3 etc 11 3a into the large holes 11 3b as follows a T...

Page 15: ...nser carrier using the drive knob 14 1 can be operated on both sides Slightly loosen the clamp screw 14 7a until the condenser can be inserted horizontally from the front Make sure the guide pin 8 3 c...

Page 16: ...l position Affix the stage to the stage bracket see above The clamp lever can be set at any position by pulling it out in an axial direction Assembly of stage object guide tube system Object guide Ass...

Page 17: ...rror and suppression filter or incident light reflector Fig 16 with the bevelled end of the dovetail guide to the front as far as the stop Up to 4 positions can be used by turning the turret plate Fig...

Page 18: ...h annular mirror Fig 16 5 are not labelled The arrow label can be used for transmitted light vacant position or for incident light brightfield POL and ICR Push the cover 35 3 hard until it clicks in p...

Page 19: ...eyeglasses it can be pushed back with eyepieces 10x 20 and 10x 22 insertable and remove pos 8a or 8b The 12 5x 16 M model is basically the same as the 10x 25 M eyepiece Assembly of graticules Only pos...

Page 20: ...he specimen stage as far as possible before assembling the objectives Attention Close vacant threads in the nosepiece with dust protection caps code no p 63 Further information p 27 28 Lamps assembly...

Page 21: ...with a 12 V 100 W halo gen lamp For incident light fluorescence mercury and xenon gas discharge lamps are preferable due to the brighter image Fig 20 24 obtained Lamphousing 106 105 2 107 2 and 107 O...

Page 22: ...mbled p 21 Check the mains voltage setting and correct if necessary or use a series transformer e g 110 230 V Lamphousing 106 105 2 107 2 and 107 Halogen lamp Disconnect from power supply mains plug m...

Page 23: ...mp holder LH 106 z only Lamphousing 106 z Hg and Xe lamps The following information is extremely important and should be adhered to under all circumstances Always unplug the power unit from the mains...

Page 24: ...and remove the lamp holder Fig 24 Remove the spent burner by loosening the clamp screws 24 1 and 24 3 Fig 24 Lamp holders for gas discharge lamps 1 Upper clamp 2 Seal point of the burner 3 Lower clamp...

Page 25: ...er ignition p 53 55 Lamphousing 106 z Hg and Xe lamps Always insert the burner so that 1 the lettering on the metal base is upright after insertion different diameters of the metal base for the Hg 100...

Page 26: ...a coverglass 0 17 The objective may only be used with a coverglass of the standard 0 17 mm thickness Use without a coverglass or with a coverglass of a very different thickness will result in a disti...

Page 27: ...n free objective for quantitative polarized light microscopy 25 RMS 32 RMS 32 25 D or A B C Pupil position of the objective important for ICT interference contrast and ICR p 48 Objective type performa...

Page 28: ...yepiece adapter tubes Not for visual observation only for adaptation of DM LD and MPS photomicro systems mounting diameter 27 mm together with special eyepiece adapter tube 46 4 HC eyepiece 18x 20 PHO...

Page 29: ...chromats N PLAN Plan achromats PL FLUOTAR Semiapochr PL APO Planapochromats Field performance of the intermediate modules The maximum admissible field performance of the intermediate modules is derive...

Page 30: ...4 Field of view 25 is only possible with direct tube adaption to microscope stand height index of intermediate modules therefore 0 providing suitable objectives are used Fov 20 and 22 can be used up t...

Page 31: ...DF Oil 6 10x 1 5 mm 1 With slot in diffusing screen but not for polarized light Lowest objective magnification for DF PH ICT 10x After removing the condenser CL PH CLP PH UCL UCLP objective magnifica...

Page 32: ...tion The total magnification of a light microscope is subject to physical limits known as the useful magnification This is roughly a thousand times the aperture of the objective p 28 Objective Eyepiec...

Page 33: ...resent to the BF brightfield position or pull out the light ring slide 7 7 Move condenser 28 4 as far as the upper stop Open the field diaphragm 28 10 If present Pull out the light trap 30 5 39 4 Set...

Page 34: ...epieces For trinocular tube with switchable beamsplitter only Set beamsplitter at visual observation by adjusting the rod 46 4 A key to the switching positions is given in symbols on the side of the t...

Page 35: ...ing glasses you should remove 18 10 for 10x 25 or push back 18 10 for 10x 20 and 10x 22 the glare protection but make sure to put it on if you are not wearing glasses 18 7 Set your interpupillary dist...

Page 36: ...and 10 4 The condenser top is swung out for objective magnifications 10x Condensers Field diaphragm Close the field diaphragm 28 10 Narrow the aperture diaphragm 28 14 if necessary Rotate the condens...

Page 37: ...ot lamp 10 Field diaphragm 11 Brightness control 12 Mount for IC prisms and compensators tube slit 13 Clamp screw for stage rotation 14 Aperture diaphragm 15 Condenser disc Fig 9 16a b Condenser centr...

Page 38: ...hragm is stopped down to less than 0 6x of the objective aperture and should be avoided where possible The aperture diaphragm is set according to the viewer s subjective impression of the image the sc...

Page 39: ...tering keys and open the field diaphragm Operation of transmitted light The lens can only be used up to an objective magnification of max 20x Exact Koehler illumination p 37 can no longer be obtained...

Page 40: ...Immersion objectives OIL only use optical immersion oil 30 4 of DIN ISO standard Cleaning p 62 Lettering p 27 28 and p 41 W Water immersion used distilled water if possible IMM Universal objective fo...

Page 41: ...ightfield Light absorbing object structures are required for this type of imaging i e it usually makes sense to stain the specimen first Optical contrasting techniques offer an alternative PH DF POL e...

Page 42: ...ting the eyelens 30 2 Retighten the clamp ring This does not apply for CL PH and CLP PH condensers 7 4 Centering the light rings Condensers UCL UCLP and UCA P Figs 7 and 10 Insert both centering keys...

Page 43: ...with higher apertures can be used if it is possible to reduce the aperture with a built in iris diaphragm These objectives can be identified by the fact that the maximum and minimum apertures are giv...

Page 44: ...ction 28 16a b until the field is as homogeneously illuminated as possible narrowing the field diaphragm again 28 10 29c You can now switch to objectives of higher magnifications Only the observed obj...

Page 45: ...le for objectives with pupil position D p 50 and 28 9 Light ring slide for CL PH and CLP PH condensers p 42 44 Assembly of polarizers Push the analyser 32 6 into the microscope stand 33 1 The engravin...

Page 46: ...trated Turn the hinged analyser outfit not illustrated into the marked centre position Remove compensators 33 4 33 7 33 8 9 6 11 10 from the light path Rotate the polarizer 33 5a or 34 8 and 34 9 unti...

Page 47: ...analyser must be pushed into the slit on the left of the illuminator not illustrated Rotatable analyser only Loosen analyser clamp not illustrated and set so that the two line markings are exactly op...

Page 48: ...n with the clamp screw 34 8 Adjustment of the condenser prisms If the equipment was delivered together the condenser prisms will already have been adjusted at the factory but it advisable to check the...

Page 49: ...onds to the magnification of the objective used e g pos 20 40 for 20x and 40x objectives note on p 15 Swing in the condenser top 0 90 S1 or 1 40 OIL Set Koehler illumination exactly p 37 This is made...

Page 50: ...f the crossed position or though crossed turned out of the zero position Polarizer has been damaged by powerful light sources Check this by holding the polarizer against a window or light source Damag...

Page 51: ...ght path 39 2 Open the light trap 39 9 Remove any diffusing screens from the light path Fig 37 Lamphousing 106 with 12 V 100 W halogen lamp 1 Screw for opening the lamphousing 2 3 x y lamp centration...

Page 52: ...the light path Switch the condenser disc to the BF position 7 3 or pull out the light ring slide 7 7 Open the aperture diaphragm in the condenser 28 14 Put a piece of paper on the microscope base Adju...

Page 53: ...alogen Xe Hg lamp Fig 40 and 41 The image of the light source is focused with the collector 40 6 if using the fluorescence illuminator you will only be able to see a few coils of the lamp filament The...

Page 54: ...hen hot Never look directly into the light path There is danger of glare when switching on the brightfield reflector BF or Smith reflector 6 4 6 5 Attention Reflector filter systems Turn an objective...

Page 55: ...possible does not apply for LH 105 2 and 107 Fig 41 Schematic diagram of lamp adjustment for lamphousing 106 z in reality the lamp images are less well defined a direct lamp image focused but decente...

Page 56: ...y improve the contrast and be gentler to sensitive fluorescence specimens Centration Put the two centering keys 2 4 into the two holes 39 5 and turn until the diaphragm is in the centre position Max f...

Page 57: ...divide the two values to obtain the micrometer value for the total magnification in use Example If 1 220 mm of the stage micrometer corresponds to 50 divisions of the measurement scale the micrometer...

Page 58: ...nd the refractive indices no of the object and ni of the medium between the coverglass and the objective air 1 d d Example The upper and lower surface of a thin polished specimen have been focused wit...

Page 59: ...ion Various adapters are available for the connection of TV cameras with c mount and B mount thread Fig 43 The c mount adapters listed in the following table can be used on all trinocular phototubes S...

Page 60: ...Vary illumination intensity carry out white balance for TV camera according to manufacturer s instructions use a conversion filter e g DLF Disturbed picture frame Remedy Earth the microscope Variotube...

Page 61: ...rease free artists hair brush or by blowing with a bellows ball or by vacuum suction Any remaining dirt can be removed with a clean cloth moistened with distilled water Failing this use pure alcohol c...

Page 62: ...ece 021 264 520 018 Eyecup HC PLAN 10x 20 eyepiece Light trap fluorescence 090 938 001 017 Light trap Incident light fluorescence Immersion oil DIN ISO standard fluorescence free 513 787 10 ml OIL and...

Page 63: ...pecial tubes enable for example graticule or slide overlay etc p 68 Attention Intermediate modules When using intermediate modules remember that the eyepiece field of view number may be affected p 30...

Page 64: ...d 100 100 4 Clamp screw Fig 46a Microscope tubes from the DM R range only with adapter 36 2 1 BSA 25 Binocular tube with focus compensation 2 HC FSA 25 PR and HC FSA 25 P Binocular phototubes with PR...

Page 65: ...sheet Viewing attachments Dual viewing attachment L 3 20 for 2 viewers Fig 48 The two viewers may either sit next to each other the image in the 2nd port is laterally reversed or opposite image positi...

Page 66: ...draw specimens by tracing their outlines or superimpose scales also lateral overlay p 68 By interrupting the microscope light path it is also possible particularly for TV microscopy to display larger...

Page 67: ...49 8 the standard 5 x 5 cm slide frame 49 6 and the control for focusing the transparencies The halogen lamp is fed by a separate transformer Fig 50 Assembly of the diapositive overlay device Align t...

Page 68: ...tc into the microscope image so that they can be recorded together with the image Attention The following diapositives are available Marker arrow 10 mm measurement scale with 100 divisions m marks for...

Page 69: ...tics 51 3 onto the tube flange 51 1 with the coupling ring 51 2 Align the macro adapter 51 5 against the macrodual zoom and secure with the threaded ring 51 6 Fasten the macro adapter and the macrodua...

Page 70: ...nation is left switched off to avoid disturbing image brightening The object is placed on the stage under the mirror housing of the macrodual zoom 51 11 and illuminated Stand lamps cold light illumina...

Page 71: ...1 17x eyepiece magnification e g 10x e g 0 11 x 1 x 1 17 x 10 1 29 The total magnification in the eyepiece would therefore be 1 29x Drawing Using the macrodual zoom as a drawing device Drawing micros...

Page 72: ...v numbers the correction value is fov 25 Auxiliary lens 2x An auxiliary lens 2x can be screwed in under the mirror 51 11 to magnify the field that is to be imaged This must be taken into account for t...

Page 73: ...nt light outfit 19 28 52 Interference contrast 15 48 Intermediate systems 12 17 30 52 64 Iris diaphragm 27 44 Koehler illumination 37 Lamps 9 21 52 63 Lamp change 21 23 26 54 Lens 2 5x 14 32 40 Light...

Page 74: ...declaration will cease to be valid if the instrument is modified without our consent Product DM LB DM LP and DM LM Model Microscope Identification Nos 020 519 010 000 020 519 011 000 020 520 701 to 02...

Page 75: ...0 64 41 29 25 99 http www leica com Leica Microsystems Wetzlar GmbH Ernst Leitz Strasse 35578 Wetzlar Tel 0 64 41 29 0 Fax 0 64 41 29 25 99 Printed on chlorine free bleached paper Order nos of the edi...

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