Lx POL
17
10
CENTRATION
PREPARATION FOR CENTRATION
1
KOEHLER ILLUMINATION CENTRATION
2
ABBE CONDENSER CENTRATION
2
9151000-795
Lx POL
Issue 1.3
Printed on April,2019
Step: 1
Disengage the Analyzer and Bertrand Lens by switching the operat-
ing lever to “O” position as shown in Fig. 13. Fully open the aperture
diaphragm of the condenser by rotating its ring to the extreme left.
Lower the microscope stage. Place a POL specimen on the stage.
Swing in the 10x objective into working position. Raise the Micro-
scope stage using the coarse adjustment knob until you reach its
positive stop. Use the fine adjustment knob to bring the POL speci-
men into focus by lowering down the stage.
Step: 2
Swing in the 10x objective into working position. Flip in top lens of
condenser in light path. Close the Koehler field diaphragm so that
its closed isirs leaves image is present within the field of view. Use
the condenser focusing knob to bring the image into sharp focus.
Operate the condenser centering screws simultaneously to Center
the image of the field diaphragm Ref. Fig 22 (a), (b), (c). After
centration open the field diaphragm until the Image (iris leaves)
disappears just beyond the field of view.
Step:3
Look through 10x Objective & Eye Pieces cross hair scale. Close the
condenser diaphragm until approximately 20-25% of the iris leaves
fill the field of view. Adjust the centering of the diaphragm by main-
taining condenser centering screws simultaneously with reference
to Eye Piece cross hair scale Ref. Fig.23 (a).
Note: When changing the objective, adjust the condenser field
diaphragm with respect to each objective.
Fig. 23
Fig. 21
Fig. 22
Fig. 23 (a)
(a)
(b)
(c)
22
22
22
