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| LABNET SPECTRAFUGE 16M MICROCENTRIFUGE
10.1 Determination of g-values
The centrifuging radius of the 1.5 mL rotor is 7.3 cm. See Section 7.1 (Rotor and Accessories) for the correct radius when using adapters
and smaller tubes. The Relative Centrifugal Force chart can be used to determine g-values.
500
475
450
425
400
375
350
325
25,000
20,000
15,000
10,000
6000
4000
3000
2000
1000
500
200
300
275
250
225
200
175
150
140
130
120
110
100
90
80
70
60
50
20,000
30,000
10,000
5000
2000
1000
500
200
100
50
20
10
0
Speed of Centrifuge
Spindle in RPM
Relative Centrifugal
Force (x g)
Radius in mm from Center of
Centrifuge Spindle to Point
Along Tube or Bottle
To calculate the RCF value at any point along the tube or bottle, measure the radius, in mm, from the center of the centrifuge spindle
to the particular point. Draw a line from the radius value on the right-hand column to the appropriate centrifuge speed on the left-
hand column. The RCF value is the point where the line crosses the center column. The nomogram is based on the formula:
RCF = (11/17 x 10
-7
) RN
2
where:
R = Radius in mm from centrifuge spindle to point in tube bottom
N = Speed of spindle in RPM