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Chapter 6: Maintaining Your Filtered PCR Enclosure
HEPA Filter Leak Test
Purpose
After installing the new HEPA filter, the HEPA filter should be
leak checked. This test is performed to determine the integrity of
the HEPA filter, the filter housing, and the filter seal. Leak testing
must be done by a qualified technician with calibrated equipment.
Remove the upper perforated diffuser located inside the enclosure
by using a Phillips screwdriver to unfasten the screws. (See Figure
6-1). The filter passes the leak test at .01% or better. Reference
Leak Testing and Photometer scanning from the Institute of
Environmental Services (IES-RP-CC001.3)
Apparatus
1.
An aerosol photometer ATI model 2D, 2E, 2G or
equivalent
. Air Techniques Hamilton Associates Inc., 11403
Cron Ridge Dr., Owings Mills, MD 21117
2. One aerosol generator of the Laskin nozzle(s) type . An
aerosol of mineral oil or suitable liquid shall be created by
flowing air through it. The compressed air supplied to the
generator should be adjusted to a pressure of 10± 1 psig. during
operation. Air Techniques Inc. Model TDA-4A or equal. One
nozzle at 10 psig is (67.5 cfm x 100ug/l)/(Vol. of air), For the
2' at 55 fpm or 230 cfm, one nozzle @10 psig is 6750/230 cfm
= 30 ug/l. For the 3' at 55 fpm or 340 cfm, one nozzle @ 10
psig is 20 ug/l. For the 4' at 55 fpm or 460 cfm, one nozzle at
10 psig is 15 ug/l.
3. Mineral oil (Catalog #1491400).
4. Sampling Nozzle, Rectangular 1/2" x 3 - 1/4", Air Techniques,
Inc.
Procedure
For the ATI 2G Photometer
1. Turn on the photometer and allow it to operate for a minimum
of 5 minutes. Leave the valve in the “CLEAR” setting.
2. Press the “ENTER” keypad. Press the “REF” keypad.
3. The display will display “P1” for approximately 1 second, and
then display a numerical value.
HEPA