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8

Useful microscopy definitions and Research Scope

options

Objective Lenses

 

There are typically three types of objective lenses found on brightfield
microscopes - 

Achromat, semi-plan, and plan objectives

.  To

understand the difference, realize that all microscope lenses are curved, which
results in an image that is slightly out of focus at the edges, and may produce
chromatic or spherical aberrations due to this curved shape.

Achromat Objective 

- An 

achromatic lens 

(

achromat

) is an objective

lens that is designed to limit the effects of 

chromatic

 and 

spherical

aberration

(See notes below). Achromatic lenses are corrected by bringing

two wavelengths (typically red and blue) into focus in the same plane.  An
objective that is Achromatic carries the implication (guarantee) that at least
60% of the field of view will have a quality flat focus.

Semi-Plan Objective 

Semi-Plan

 objectives are guaranteed to have a

quality flat field of focus for 80% of the field of view.  A

 Semi-Plan

Achromat

lens is also corrected for chromatic aberrations, as described

above.

Plan Objective

 - Plan Objectives guarantee a quality flat field of focus for

100% of the field of view.  A 

Plan Achromat

 lens will also have the same

corrections for chromatic aberrations as noted above.  In Plan Objectives
when imaging the field, parts of the specimen at the edge of the visual field

are almost as well focused

 as those in the center. Straight lines on the

specimen appear straight throughout the visual field, without any aberrations.
Such microscopes are especially good for photomicrography.  In general, the
better the plan field objective, the more expensive the lens.

Flat Field Objectives

 - As one looks at the field of view, there may be slight

blurring at the edge of the field.  This edge blurring of 

Flat Field

 objectives is

slight and easily corrected by a small tweak of the fine focus knob as one looks
toward the edge of the field, or by moving the specimen so that the portion
that is at the edge is moved into the center of the viewing field.  Flat Field
Objectives tend to be inexpensive when compared to Plan Field.

Plan Field versus Flat Field Objectives 

-   Most educational, teaching

microscopes are Flat Field Objectives due to this cost difference.

Infinity - Infinity lenses 

- In microscopy, the term "infinity" means that the

objective lenses are designed to project their images to infinity, not to some
finite distance.  Traditional brightlight microscopes had a "fixed" focal point at

Содержание Research Scope T-29031

Страница 1: ...cope T 29033 Binocular Acromat Plan Research Scope T 29043 Trinocular Acromat Plan Research Scope T 29034 Binocular Infinity Achromat Research Scope T 29044 Trinocular Infinity Achromat Research Scope T 29035 Binocular Infinity Semi Plan Research Scope T 29045 Trinocular Infinity Semi Plan Research Scope T 29036 Binocular Infinity Plan Research Scope T 29046 Trinocular Infinity Plan Research Scope...

Страница 2: ...2 ...

Страница 3: ...rs can operate the instrument conveniently and safely T 29031 T 29041 T 29032 T 29042 T 29033 T 29043 Halogen Light 12V 20 W D D D D D D High Bright Köhler Illumination D D D D D D Low Coaxial Fine Focus Scale 0 002mm D D D D D D 30 Inclined Binocular 55mm 75mm D D D 30 Inclined Trinocular 55mm 75mm D D D Eyepieces 10X D D D D D D DIN 4X 10X 40X 100X Oil Objectives Achromat Achomat Semi Plan Achom...

Страница 4: ...inocular 55mm 75mm D D D 30 Inclined Trinocular 55mm 75mm D D D Eyepieces 10X D D D D D D DIN 4X 10X 40X 100X Oil Objectives Achomat Infinity Achomat Infinity Semi Plan Infinity Semi Plan Infinity Plan Infinity Plan Infinity Double layered mechanical stage 180mm x 150 mm D D D D D D Gimbaled N A 1 25 Abbé Condenser with built in Iris Diaphragm and filters for Köhler Illumination D D D D D D ...

Страница 5: ...e manipulated by moving the gimbaled condenser by using the three substage manipulating screws High Bright Köhler Illumination Köhler Illumination Defined Köhler illumination is a method to reduce an old problem of transmitted light microscopes The filament of illuminating bulb of the microscope is often visible in the same plane as the sample reducing resolution and sharp focus One simple partial...

Страница 6: ...ntrast can introduce artifacts into your view and misrepresent what you actually see in the specimen Finally adjust the light intensity to your comfort level If available it is bet ter to use a neutral density filter in the filter holder below the bottom of the condenser then severely reducing the light intensity A neutral density filter tends to block all wave lengths equally Reduction of volt ag...

Страница 7: ...l a suitable position is found The stage should not move on its own as it sits waiting for your next use Bulb replacement Replacement bulb is Ken A Vision Part T6V20WP IMPORTANT NOTE Before replacing the lamp turn OFF the power switch and unplug from electrical source Place microscope on its side or back exposing the bottom of the unit Unscrew the knurled knob that is towards the front of the unit...

Страница 8: ... 100 of the field of view A Plan Achromat lens will also have the same corrections for chromatic aberrations as noted above In Plan Objectives when imaging the field parts of the specimen at the edge of the visual field are almost as well focused as those in the center Straight lines on the specimen appear straight throughout the visual field without any aberrations Such microscopes are especially...

Страница 9: ...lurry in places Spherical aberrations Spherical aberration occurs when the rays of light passing through the center of the lens tend to focus on a different plane than those entering near the edge of the lens This is because the curvature of the lens is not perfect The variance in cost of microscopes in general is often attributed to the cost of lenses needed to cure this problem Often the only wa...

Страница 10: ...avoid storing unit where it may be exposed to any chemical volatiles or other airborne corrosives Long Term Maintenance All optical and mechanical equipment requires periodic servicing to keep it performing properly Ken A Vision recommends the user anticipate this need by establishing a schedule of regular preventive maintenance to help to assure long life and sustain optimum performance of your i...

Страница 11: ...ssories If you are interested in purchasing accessories or replacement parts for your Research Scope visit the Ken A Vision Web site http store kenavision com catalog or contact your local Ken A Vision Dealer ...

Страница 12: ...en a vision com www ken a vision com INS SC29 Ken A Vision reserves the right to make design improvements and other changes in accordance with the latest technology There is no obligation to make changes in products already manufactured Patents Pending Copyright 2009 Ken A Vision Corporation ...

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