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The dots represent individual amplification curves in your
experiment. All dots that are within the top right of the
graph are called positive and will be subject to normalization
determined in Advanced Settings.
To switch between “Intensity vs Quality” and “Performance
vs Quality” select the Graph Axes drop down menu. All 3
thresholds are used for calling even if they are not currently
displayed on the graph.
SETTINGS
The software uses a combination of Quality (Q), Performance
(P), and Intensity (I) thresholds to determine if an amplification
is positive (green zone) or negative (red zone). All values (Q, P
and I) must be above the relevant threshold for an amplification
to be called positive. The Settings tab enables you to review
metrics for amplifications and set appropriate thresholds.
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