Invitrogen Bandmate MAN0018722 Скачать руководство пользователя страница 13

Observation

Possible cause

Recommended action

Processor stopped working.

Processor electronics errored.

Turn the system off for one minute to allow
the electronics to reset. If the processor does
not turn on after resetting, contact technical
support.

Processor loses power.

Processor was unplugged or a
power outage occurred.

Restore power to the unit when possible. If
a run was in progress, it will automatically re-
start at the point it had reached when power
was lost (only if the program was saved). No
other special measures are needed. If you
wish to terminate the recovered run, press
the "home" button when the program recovery
screen is displayed.

Processor stops during a run

and displays an error message.

An error was detected. The
most common error is that the
work area window is not fully
closed.

The run will resume once the error is
corrected.

Antibodies are not dispensed.

The pokers on the sample tray
are not facing toward the back
of the instrument.

Ensure that the pokers on the sample tray are
facing toward the back of the instrument.

The pokers on the sample tray
have been damaged.

Ensure that the pokers on the sample tray
remain at a 90° angle to the horizontal, are
not bent over, and that they are still sharp.
If the pokers are bent, it may be possible to
bend the pokers back to their original vertical
position. Test the tube-puncturing capability of
the pokers with water or buffer in the reagent
tubes after repositioning.
Repeat the run with a new sample tray that
is provided with the processor. Alternatively,
replacement trays are available for puchase if
the pokers become damaged.

Buffer is not dispensed during

the run.

The end of the buffer hose
may not have been resting at
the bottom of the filled buffer
bottle, or the cap may be tight
and not allowing air flow into
the bottle.

Ensure that the end of the buffer hose is
resting in the bottom of a filled bottle of buffer.
The buffer bottle cap should be slightly loose
to allow air to flow into the bottle.

The device is noisy.

The tray’s magnet covers were
omitted, or the trays were not
correctly seated on the rocker
cradle. The omission of the
magnet cover may lead to the
tray bouncing out of the rocker
cradle.

Before starting the program, ensure that both
magnetic cover trays are securely in place and
correctly aligned on the blot trays.

Chapter 1 

Bandmate

 Automated Western Processor

Troubleshooting

1

Bandmate

 Automated Western Blot Processor User Guide

13

Содержание Bandmate MAN0018722

Страница 1: ...ch Use Only Not for use in diagnostic procedures Bandmate Automated Western Blot Processor USER GUIDE For automation of western blot processing Catalog Numbers BW1000 Publication Number MAN0018722 Rev...

Страница 2: ...sion history Pub No MAN0018722 Revision Date Description C 0 14 Sep 2021 Added to products for separate purchase and troubleshooting topics Minor corrections B 0 06 Oct 2020 Corrected safety and regul...

Страница 3: ...connection symbols 16 Conformity symbols 16 Safety information for instruments not manufactured by Thermo Fisher Scientific 17 Instrument safety 17 General 17 Physical injury 18 Electrical safety 18 C...

Страница 4: ...mes Antibodies and reagents are directly streamed into incubation trays which eliminates cross contamination between reagents The curved mini and midi trays allow for even processing of blots with min...

Страница 5: ...or Siphon Hose for Buffer Bottle 1 Reagent Tubes 40 50 mL Recovery Tubes 8 Mini Trays 2 Midi Trays 2 Tray Covers 2 Funnel Trays 2 Waste Trays 2 Quick Reference Guide 1 Table 3 Available for separate p...

Страница 6: ...le Protein Stain Kit for PVDF Membranes 24585 Operate the Bandmate Automated Western Blot Processor Set up the system 1 Set up the processor on a stable level lab bench 2 Open the door and remove the...

Страница 7: ...and as a template Select the desired program using the dial Press Edit 2 Enter a name for the custom protocol Select letters and numbers by turning the dial Use the cluster of 4 buttons at the lower l...

Страница 8: ...if antibodies need to be saved Table 6 Standard Midi Step Reagent Repeat Fill volume Rock time Rocks per min Drain to 1 1 Pre Fill 1X 1 hr 15 Waste 2 1 1X 1 hr 15 Waste 3 Buffer 3X 40 mL 10 mins 20 W...

Страница 9: ...two reagent tubes will be required for each blot being processed Check to ensure the foil on the bottom of the reagent tubes is not damaged Screw one reagent tube firmly and completely into the prima...

Страница 10: ...tinue to rock with the final dispensed wash buffer volume in the tray after all of the steps of the protocol are completed to ensure that the blots do not dry out The Home button should be selected be...

Страница 11: ...the pump has stopped running measure the actual amount of liquid dispensed and enter the value using the encoder knob Measure the volume from the other positions in the same way and record the value P...

Страница 12: ...um washes per step 10 Minimum reagent per blot 3 5 mL mini 7 5 mL midi Maximum number of protocol steps 9 Maximum number of protocols 7 Buffer dispensing volume 10 150 mL Incubation step time range 0...

Страница 13: ...the sample tray remain at a 90 angle to the horizontal are not bent over and that they are still sharp If the pokers are bent it may be possible to bend the pokers back to their original vertical pos...

Страница 14: ...will appear The reagent tubes were not pierced Possible issues can include a faulty tube bent piercing points on the incubation tray or faulty alignment of the reagent slider Verify that all tubes hav...

Страница 15: ...see the Documentation and Support section in this document Symbols on this instrument Symbols may be found on the instrument to warn against potential hazards or convey important safety information In...

Страница 16: ...s conformity with European Union requirements Indicates conformity with Australian standards for electromagnetic compatibility Indicates conformity with the WEEE Directive 2012 19 EU CAUTION To minimi...

Страница 17: ...and Pressurized fluids Wear eye protection when working with any pressurized fluids Use caution when working with any polymeric tubing that is under pressure Extinguish any nearby flames if you use fl...

Страница 18: ...and hold it stationary while someone else slides the contents out of the box Electrical safety WARNING Do not overfill trays with liquid Excess liquid can overflow into the control unit and possibly...

Страница 19: ...re recommended by the manufacturer confirm with the manufacturer that the proposed method will not damage the equipment Instrument component and accessory disposal To minimize negative environmental i...

Страница 20: ...igital device pursuant to part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference in a residential installation This equipment generates uses a...

Страница 21: ...trical and electronic equipment SJ T 11364 2014 China RoHS Standard Marking for the Restricted Use of Hazardous Substances in Electronic and Electrical Products For instrument specific certificates vi...

Страница 22: ...primary waste container holds the immediate waste A secondary container contains spills or leaks from the primary container Both containers must be compatible with the waste material and meet federal...

Страница 23: ...goggles Individuals should be trained according to applicable regulatory and company institution requirements before working with potentially biohazardous materials Follow all applicable local state...

Страница 24: ...tocols Certificates of Analysis Safety Data Sheets SDSs also known as MSDSs Note For SDSs for reagents and chemicals from other manufacturers contact the manufacturer Limited product warranty Life Tec...

Страница 25: ......

Страница 26: ...ated Western Processor_UG_MAN0018722 v6 GUID 8DBD70A8 7BC4 4AB0 919A 5601AB0A9781 2021 07 19 19 26 41 en 15 09 51 386 01 00 thermofisher com support thermofisher com askaquestion thermofisher com 14 S...

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