Invitrogen Bandmate MAN0018722, Руководство пользователя

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9.
To recover the antibody solutions, place empty 50 mL uncapped conical tubes in the appropriate
spaces on each side of the waste tray (space #1 for the primary antibody and #2 for the secondary
antibodies).
Note: For antibody recovery from midi trays, conical tubes must be added on both sides of the
waste tank as recovered solutions will be distributed in both tubes.
10.
Check the waste tank to make sure that it is empty. Ensure that the funnel tray and waste trays are
pushed all the way to the back and are in place. Close the waste compartment and the work area
window.
11.
Select the desired protocol from the menu and press Run .
12.
Select the trays to be used for the protocol. Follow the instructions on the screen to indicate which
section(s) are in use.
13.
Press Continue .
14.
Press Run .
15.
Once the run is complete, press Home to stop the rocker and return to the home screen.
Open the window and remove the blot. The blot is now ready for downstream processing
(chemiluminescent or colorimetric detection) and/or immediate imaging (fluorescence).
Note:
The rocker will continue to rock with the final dispensed wash buffer volume in the tray after
all of the steps of the protocol are completed to ensure that the blots do not dry out. The Home
button should be selected before removing the blot from the blot tray.
Clean the processor
1.
Wash the sample trays, funnel trays, and tray covers using a gentle laboratory detergent. Rinse
with distilled water and dry face down.
2.
Remove the Reagent Tubes from the instrument.
3.
If the processor is not going to be used again for some time, flush the buffer line with distilled
water to prevent salt build-up. To flush, place the wash buffer hose into a container of distilled
water, then select
Flush to run water through the lines and into the trays. Once water has been
flushed through the tubing, remove the tubing from the water container, then select Flush until the
liquid is completely flushed from the system and tubing.
General maintenance
Replacing buffers
When replacing or adding additional wash buffer to the wash bottle, it is recommended to flush the lines
to ensure no bubbles were introduced when the buffer was replenished.
Chapter 1
Bandmate
™
Automated Western Processor
Operate the Bandmate ™ Automated Western Blot Processor
1
10
Bandmate ™ Automated Western Blot Processor User Guide