41
Principal Reason for Performing the Test
As a measure of glomerular filtration rate.
Most Common Abnormality Indicated by the Test
Increased inorganic phosphate—decreased glomerular filtration.
Sample Type and Precautions
Remove plasma or serum promptly from the cells or clot. If plasma is being collected, use only
lithium heparinized samples. Do not use oxalate, fluoride, citrate, or EDTA as anticoagulants. Blood
samples must be processed and centrifuged as soon as possible after collection as phosphates
are released quickly from the red blood cells. Hemolysis can result in marked increases in
phosphate concentration.
Complementary Tests
Inorganic phosphate determination should be performed in conjunction with measurements
of calcium, albumin, total protein, and glucose. If renal disease is suspected, BUN, creatinine,
albumin, total protein, and a complete urinalysis should also be determined.
Reaction Sequence
Lactate Dehydrogenase (LDH)
The enzyme lactate dehydrogenase is present in large amounts in all organs and tissues (including
red blood cells) of most animals. It is found in the cell cytoplasm and is released into the blood
during reversible and irreversible (necrosis) cell injury. The test is not a specific or sensitive indicator
of damage to any organ or tissue.
Note:
The normal range of lactate dehydrogenase in the dog and cat is wide, as can be the
intra-animal variation from day to day. Consequently, small increases in activity due to minimal
organ damage are difficult to identify. The measurement of lactate dehydrogenase is a somewhat
traditional test whose diagnostic value is limited in practice.
Principal Reason for Performing the Test
To investigate damage to liver, cardiac or skeletal muscle.
Most Common Abnormality Indicated by the Test
Increased activity is usually associated with hepatic parenchymal lesions.
Sample Type and Precautions
Remove plasma or serum promptly from the cells or clot and analyze as soon as possible. If
plasma is being collected, use only lithium heparinized samples. Fluoride/oxalate and EDTA should
not be used as anticoagulants.
Hemolyzed specimens should not be used because LDH contamination from red blood cells will
occur.
Complementary Tests
Lactate dehydrogenase activity is usually determined in conjunction with other tests of liver, cardiac,
or skeletal muscle function or damage.
Appendices
Содержание Catalyst One
Страница 1: ...IDEXX Catalyst One Chemistry Analyzer Operator s Guide...
Страница 53: ...53 Appendices...
