ibidi 12170, Инструкция по эксплуатации

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Objective Heater Universal – Silver Line
5.2 Emergency Release Mechanism
A break-away connector in the cable between the Objective Heater and the Temperature Controller
allows for emergency release of the Objective Heater from the Temperature Controller (see Figure
3 ). If a force above about 14 N acts on the cable of the Objective Heater, the break-away connector
releases open to prevent any force from being transferred to the microscope’s objective.
Figure 3: Principle of the emergency release mechanism by a break-away connector in the cable
between the Objective Heater and the Temperature Controller.
5.3 Temperature Adjustment of the Objective Heater Universal – Silver Line
ibidi recommends a temperature adjustment before starting the first experiment to ensure that the
sample reaches the correct temperature. The temperature in the sample is affected primarily by the
temperature of the Stage Top Incubator. In addition, the temperature of the microscope objective
can contribute to the sample temperature by heat transfer from the sample to the objective and vice
versa. The temperature of the Objective Heater is regulated by the Temperature Controller. Please
follow the instructions for temperature adjustment of the sample as described in the instructions of
the Temperature Controller (
see Instructions ).
1. Adjust temperatures of Heated Plate and Heated Lid in a way that the target temperature for
the sample is reached (e.g., 37°C), while the objective is not yet in contact with the sample.
2. As soon as the target temperature of the sample is reached, bring the cold objective in contact
with the sample. The temperature of the sample will most likely drop. Wait until the tempera-
ture of the sample reaches a stable plateau.
3. In order to bring back the sample temperature to the target temperature, heat up the Objective
Heater to an initial temperature between 38°C and 45°C. The optimal temperature depends on
the diameter and material of your objective. Wait until the temperature is stable again.
4. Adjust the temperature of the Objective Heater, if necessary. We recommend beginning the
temperature adjustment with a low temperature and to increase the temperature stepwise by
0.5–1 °C.
Version 1.0 (June 1, 2022) 16 Instruction Manual