ibidi 12150, Инструкция по эксплуатации

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ibidi Heating System Multiwell Plate – Silver Line
2 Intended Use
The ibidi Heating System Multiwell Plate – Silver Line is a stage top incubator for live cell imaging
that fits onto inverted microscope stages with a mounting frame K opening (110 × 160 mm). The ibidi
Heating System Multiwell Plate, consisting of an Incubation Chamber with Heated Plate and Heated
Lid and a Temperature Controller, provides a platform for keeping multiwell plates for microscopy
at a constant temperature during an experiment on a microscope.
For full control of the incubation environment, the ibidi Heating System Multiwell Plate can be com-
bined with the ibidi Gas Incubation System to regulate the CO
2
and O
2
concentrations as well as the
humidity.
The ibidi Heating System Multiwell Plate is for research use only! It is not intended for use in diag-
nostic procedures.
3 Principle
Physiological Conditions in Live Cell Imaging
Cells react sensitively to changes in their environment. Factors such as temperature, humidity, and
CO
2
/O
2
levels significantly influence the outcome of cell culture assays. In order to achieve biologi-
cally relevant and reproducible results, it is crucial to maintain optimal conditions on the microscope
stage during live cell imaging experiments.
To enable high-quality live cell imaging under physiological conditions, the following parameters
need to be considered:
Environment:
• Temperature
• Carbon Dioxide (CO
2
) Levels and pH of the
Medium
• Humidity and Evaporation
• Oxygen (O
2
) Levels
Optical Aspects:
• Condensation
• Imaging Method and Photon Dose
Figure 1: Live cell imaging conditions.
The Heating System combined with the Gas Incubation System supplies complete incubator condi-
tions for your live cell imaging directly on the microscope stage.
Version 1.0 (August 1, 2022) 14 Instruction Manual