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3

Measurement (calibration)

1. Open the internal solution filler port.

2. If the amount of internal solution has decreased, fill up

the internal solution.

3. Move the sleeve, and check that internal solution drains.

And then push down the sleeve lightly.
If the sleeve is fixed, immerse it in warm water (30°C to
40°C) for tens of minutes.

4. Wash the electrode with pure water (or deionized water)

and wipe it with filter paper or tissue paper, prior to every
immersion into standard/sample solution.

5. When there are air bubbles inside the responsive glass

membrane, stir it lightly to remove bubbles.

6. Immerse the responsive glass membrane and liquid

junction in the sample completely. 
Make sure that the surface of the internal solution inside
the electrode is higher than the surface of the sample
solution.

Bubbles

Liquid junction hole

7. Stir the electrode lightly to remove bubbles.

Note

Be sure to keep the internal solution filler port open during
measurement. If it is clogged with the solution, accurate
measurement cannot be performed. Wipe the solution with
cloth.

For calibration/measurement on pH meter, refer to the
instruction manual of pH meter.

Maintenance

Note

Do not use organic solvents (such as acetone or
tetrahydrofuran) or acid (above 1 mol/L) or alkaline (above 0.1
mol/L) cleaning solutions. Using such chemicals may damage
the body of the electrode or cause a decline in performance.

To maintain electrode performance, replace the internal
solution approximately every one or two months. (Refer
to "Preparation" (page 2).)

Dirt on the responsive glass membrane or liquid junction
by the sample may cause a decline in electrode
response and sensitivity or measurement error. If the
electrode is very dirty and cannot be washed clean
using pure water (or deionized water), perform the
following cleaning.

Cleaning of electrode

1. Open the internal solution filler port.
2. Immerse the responsive glass membrane and liquid

junction in cleaning agent according to the type of dirt for
approximately one hour until contamination is removed. 
Refer to the instruction manual of cleaning agent.
The responsive glass membrane can also be cleaned by
wiping it with cotton gauze containing cleaning agent.

3. Rinse the electrode with pure water (or deionized water)

completely.

Type of dirt

Cleaning agent

General

Diluted neutral cleaning agent

Oil

Alcohol, or diluted neutral cleaning agent

Inorganic substance

Electrode cleaner (model 220) or Hydrochloric 
acid approximately 1 mol/L

Protein

Cleaning agent including protein-cutting enzyme 
(model 250)

Liquid junction

Electrode cleaner (model 220)

Altered of glass, 
Slow-response

Electrode cleaner (model 220)

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