6
12. Place the 12-in-1 rack into the instrument as shown in the below fig. and secure the rack in
position by two lock plates aside the worktable.
Fig. 10
13. Prepare sample with proper Pre-treatment
Sample Pre-treatment procedure
Weigh 150 mg of the fungal material and add to the HiBead tubes (DBCA04).
Add 400
l of Lysis Solution (PL) (DS0016)
.
Vortex vigorously for 5 minutes.
Add 20
l of RNaseA Solution and incubate the mixture for 10 minutes at 65°C.
Centrifuge the samples at 14,000 rpm for 1 minute at room temperature (15-25°C).
Transfer the supernatant to a new collection tube and add 130
l of Precipitation Buffer
(PS) (DS0017). Mix and incubate in ice for 5 minutes.
Transfer the entire lysate on to the HiShredder (DSCA02) and centrifuge the samples at
14,000 rpm for 1 minute at room temperature (15-25°C).
Transfer the flow through without disturbing the pellet to a new Collection tube (provided)
Proceed with step 14 for DNA purification in Insta NX® Machine as follows.
14. Place the Collection tube containing sample onto the 12-in-1 rack.
Fig. 11
