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5. On the

Product Type

screen, select the type of purification to be carried out.

Fig. 4

6. On the Setting screen

a. Select Elution volume: 50/ 100/150 or 200µl
b. Select Kit Name: Select the type of extraction procedure to be performed.

Fig. 5

7. Lift open the front door and take out the 12-in-1 rack for preparation.

NOTE:

You can follow

Step by Step Setup worktable

for preparation of 12-in-1 rack

8. Place the Fungal DNA Extraction Cartridges (DS0401) on 12-in-1 rack as shown in the

following figure.

Fig. 6

MBIN008 Insta NX® Fungal DNA

Содержание Insta NX

Страница 1: ...uch as PCR Southern blotting technique etc The DNA purification procedure using the column comprises of three steps viz adsorption of DNA to the Super S membrane removal of residual contaminants and e...

Страница 2: ...etween 15 25 C except certain components as specified on each labels Under recommended condition kit is stable for 4 months Materials needed but not provided Tabletop Microcentrifuge with rotor for 2...

Страница 3: ...e 1 Turn on Insta NX instrument Allow the instrument to initialize 2 Once the instrument is ready select Login on the instrument screen Fig 1 3 Enter the Password to login Fig 2 4 On the Home screen s...

Страница 4: ...t Kit Name Select the type of extraction procedure to be performed Fig 5 7 Lift open the front door and take out the 12 in 1 rack for preparation NOTE You can follow Step by Step Setup worktable for p...

Страница 5: ...e cartridge as shown below Fig 7 10 Place 1ml tip DS0337 set onto the 12 in 1 rack Fig 8 11 Open the metal lid and place the Collection tube Polypropylene PW1139 into 12 in 1 rack as shown in figure b...

Страница 6: ...ure for 10 minutes at 65 C Centrifuge the samples at 14 000 rpm for 1 minute at room temperature 15 25 C Transfer the supernatant to a new collection tube and add 130 l of Precipitation Buffer PS DS00...

Страница 7: ...ile the run is going on Fig 13 17 After the run is completed the screen will look like the below figure Fig 14 18 Lift the instrument lid and take out the 12 in 1 rack Remove the elution tubes and dis...

Страница 8: ...d the procedure carefully before starting the experiment Performance and Evaluation Each lot of Insta NX Fungal DNA Purification Kit is tested against predetermined specifications to ensure consistent...

Страница 9: ...claving and or incineration of used or unusable preparations of this product Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with cli...