HEKA Elektronik EPC 10 USB, Руководство по оборудованию, Страница: 25 / 81

4.1 Testing the EPC 10 USB with the Model Circuit 19
1 : SETUP
E Reset ;
E Mode 3 ; Whole Cell
E PulseAmp 5.0mV ;
E PulseDur 5.0ms ;
E CSlow 50.00pF ;
E RSeries 20.0MOhm ;
E Gain 10 ; 5.0 mV/pA -> medium range
E AutoZero ;
E PulseOn TRUE ; Switch on test pulse
E SaveRpip ;
E Beep ;
Note:
Patchmaster
has a built-in macro interpreter that executes command lines of the form ”
Window
Control[: parameter; comment]
”. E.g., the line ”
E Gain: 10
” would instruct
Patchmaster
to
set the gain pop up in the
EPC 10 USB
window to the 10th value (5 mV/pA). A list of all so-called
Macro Items can be output into the Notebook via the
Help
menu of
Patchmaster
.
The predefined protocols are stored in a text file called
*.pro
and can be edited with any text editor. For
this tutorial it is not necessary to know all possible commands and their syntax. Therefore, please, refer to the
Patchmaster
manual for a detailed description on how to record and modify protocols in the Protocol Editor .
4.1.3 ”SEAL” - Voltage Clamp Recording
Now move the switch of the model circuit to the center position which leaves only a capacitance of about 6 pF
connected. This simulates a GΩ seal and the
C-fast controls can be used to cancel the capacitive spikes resulting
from the stimulus Test Pulse .
Figure 4.3: Before C-fast compensation
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