5
Publication 5502-018-REV 1.0
1. Prior to use of electrode, spray with
Rain-X and gently shake over platinum
electrode surface of electrode for
10 seconds. Discard Rain-X. Sterilize
Oocyte Petri Electrode by spraying
three times with 75% ethanol, then
drying with a Kimwipe. Rinse with
sterile MilliQ water, then dry with a
Kimwipe. Electrode is now ready to use.
Note: Sigmacote may be used as an
alternative to Rain-X to coat electrodes
prior to use.
2. Connect the Mini Micro-Grabber cables
to the Oocyte Petri Electrode, plug the
banana cable into the Tweezertrode
Adapter Cables and then connect the
Adapter Cables into the voltage output
of the BTX Electroporator.
3. Following instructions for the BTX
Electroporator, set the appropriate
parameters for electroporation.
4. Use a wide bore pipette tip to transfer
oocytes or zygotes to the 1 mm gap
between the two electrodes on the
slide. Suggested fill volumes are 7 µl
for 70 embryos and 10 µl for 100–120
embryos.
5. Deliver the electroporation pulse(s) to
the sample.
6. Recover sample with wide bore
pipette tip. During a session of
multiple electroporations using the
same transfectant, excess buffer may
be removed with a sterile eye spear in
between rounds of electroporation.
7. Disconnect from Mini Micro-Grabber
cables and clean electrode by flushing
with distilled water.
8. Sterilize Oocyte Petri Electrode by
spraying with 75% ethanol three times
and allow to dry.
9. Electrode is ready for next sample.
10. If finished with electroporation,
remove Mini Micro-grabber cables
from Oocyte Petri Electrode.
11. Gently flush electrode gap with
a mild detergent and rinse with
distilled water.
12. Dry electrode with 75% ethanol by
flushing electrode with alcohol and
gently shake any remaining drops.
13. Allow to air dry until no alcohol is seen
and store with glass cover in a dry
area.
Make sure the BTX Electroporator is switched off before continuing.
WARNING: High Voltage
Operation: Getting Started
