ZERO
5
1
3
4.0
ml
Sample
2
4
15
min
PROCEDURE
for Free Iron
applies to DR/4000, DR/2500 DR/2010 and DR/800
Note
If this is the first time you have run this revision of the test on
your DR/4000, you must contact your local
Hach
distributor
for an upgrade of your software (Cat.No. 49544-00). Software
Version number 2.39 contains the factory
UniCell™
programs.
INSTRUMENT SET-UP
1. Press the soft key under
HACH PROGRAM
(or
USER PROGRAM
if the method was entered as a
USER PROGRAM
).
Enter program number
2130
(or
159
if the method was
entered as a
USER PROGRAM
).
Press
ENTER
.
2. The display will show:
HACH PROGRAM: 2130
Iron, HCT 159
The wavelength (
λ
),
485 nm
, is automatically selected.
3. Insert the
Test N’Tube
adapter into the cell holder by
sliding it under the thumb screw and into the alignment
grooves. Fasten in place by tightening the thumb screw.
4. Follow the procedure steps.
(
Note:
If measuring Total Iron and performed the sample
digestion using the HCT 200 Metal Prep Set, select the
Total Iron alternate form under the Options soft key menu).
5. Wipe the outside of the zero vial.
Place the zero vial into the adapter.
Close the light shield.
6. Press the soft key under
ZERO
.
The display will show:
0.00 mg/L Free-Fe
or
0.00 mg/L Tot-Fe
if the Total Iron alternate form
was selected.
7. Clean the outside of the sample vial.
8. Place the prepared sample vial into the adapter. Close
the light shield. Results in
mg/L Free-Fe
or
Tot-Fe
are
displayed.
Note
If the test overranges, repeat the
UniCell™
test with diluted
sample.
2
. Pipet
4.0 ml
sample
into the sample vial.
3
. Close sample vial and
invert a few times, until
the freeze-dried
contents are
completely dissolved.
4
. Start the instrument
timer.
A
15-minute
reaction
period will beginn.
5
. Zero the instrument
using the zero vial
(white cap) provided
and read the sample.
DR / 4000—Rev 2.39
1
. Enter program number
and set up the
instrument as explained
for each instrument
type.
HCT 159
HCT 159
LIST OF REQUIRED REAGENTS
Iron-Fe
UniCell™
HCT 159
23/pkg
HCT 159
OPTIONAL REAGENTS
Metal Prep Set
UniCell™
HCT 200
50 digestions HCT 200
Iron Standard
1000 mg/L as Fe
100 ml
2271-42
Sodium Hydroxide
Standard Solution
1000 ml
2450-53
(5.0 N)
Nitric Acid, concentrated
500 ml
152-49
OPTIONAL APPARATUS
Cylinder, mixing – 100 ml
each
20886-42
Flask, volumetric 100 ml
each
547-42
Pipettor, (Jencons) 1-5 ml
each
27951-00
Replacement tips for 27951-00
pk/100
27952-00
Pipettor, (Jencons) 100-1000 µl
each
27949-00
Replacement tips for 27949-00
pk/400
27950-00
pH Paper
pk/100
26013-00
MEASURING RANGE:
0.10 - 5.00 mg/L Free-Fe / 0.12 - 6.00 mg/L Tot-Fe
PROCEDURE
for Total Iron:
After digestion with the Metal Prep Set HCT 200,
follow the procedure steps 1 – 5.
