GenScript eStain 2.0 Скачать руководство пользователя страница 1 | Manualshive

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Aug 15, 2014

GenScript USA Inc.

860 Centennial Ave.

Piscataway, NJ 08854

Tel: 732-885-9188, 732-885-9688

Fax: 732-210-0262, 732-885-5878

Email: [email protected]

For fast Coomassie blue staining of proteins in mini polyacrylamide gels

2.0

®

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Содержание eStain 2.0

Страница 1: ...ript USA Inc 860 Centennial Ave Piscataway NJ 08854 Tel 732 885 9188 732 885 9688 Fax 732 210 0262 732 885 5878 Email product genscript com For fast Coomassie blue staining of proteins in mini polyacr...

Страница 2: ...cifications eStain 2 0 Protein Staining Device Introduction Quick Reference Guide Protocols Troubleshooting Examples of Results Technical Support Warranty Appendix 1 3 5 7 10 11 20 22 23 24 25 eStain...

Страница 3: ...rom GenScript If you order the eStain Protein Staining Pads you will receive the components listed in the table below Store the eStain Protein Staining Pads at room temperature For best results use th...

Страница 4: ...esoaked with proprietary anode buffer The eStain Protein Staining Pads are used with the eStain 2 0 Protein Staining Device The specifications of the eStain Protein Staining Pads are listed below eSta...

Страница 5: ...staining mode when both left and right status lights are on the device is working at numbering mode The Reset button is used to clear parameters The Min button is used to shift between staining and n...

Страница 6: ...ning of proteins in gels with Coomassie blue dye Two types of eStain Protein Staining Pads R 250 and G 250 are available separately from GenScript and for different requirements Each pack of eStain Pr...

Страница 7: ...2 2H2 O 4e Quick Reference Guide Mode Sponge cushion and graphite electrode installed inside eStain 2 0 Device eStain 2 0 Device plugged in eStain 2 0 Device and staining stack assembled Time selectio...

Страница 8: ...fied expiration date printed on the package Protocols continued Installing the eStain 2 0 Device continued Note For a new eStain 2 0 Protein Staining Device two pieces of Sponge Cushion and one unit o...

Страница 9: ...n Anode Pad package Remove the eStain Anode Pad from the package and place it on the anode plate of the eStain 2 0 Protein Staining Device 5 Place the gel on the eStain Anode Pad Gently remove air bub...

Страница 10: ...clean the device To obtain consistent staining results disassemble the staining stack right away after ending the staining procedure 1 Open the closed lid by pressing the Open button 2 Carefully sepa...

Страница 11: ...ter than 100 replace the eStain 2 0 Graphite Electrode as described below Protocols continued During electric staining process the eStain 2 0 Graphite Electrode will absorb ions from anode pad while l...

Страница 12: ...k if the staining stack is properly assembled and ensure full coverage of the gel 1 Cover the eStain Cathode Pad again and then perform electric staining for 1 or 2 more minutes 2 Replace the worn eSt...

Страница 13: ...20 ng reduced BSA Lane 9 10 l New England Biolabs Protein Ladder Cat No P7703S Lane 10 5 l New England Biolabs Protein Ladder Cat No P7703S 1 2 3 4 5 6 7 8 9 10 Troubleshooting continued Problem Ring...

Страница 14: ...oper solvent or sample For any inquiry or request for repair service contact GenScript after confirming the model and serial number of your instrument For your protection items being returned must be...

Страница 15: ...eStain 2 0 Protein Staining System www genscript com eStain 2 0 Protein Staining System www genscript com 26 25 Appendix Appendix continued...

Страница 16: ...eStain 2 0 Protein Staining System www genscript com 27 Appendix continued...

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