4. Operation
4.6 Basic
operating
procedure -
AU
4.7 Basic
operating
procedure -
Transmission
(%T)
4.8 Shut down
1.
2.
3.
4.
1.
2.
3.
4.
5
.
6.
7.
1.
Switch AU/ %T to AU (Fig.
5
:13).
Set the range selector (Fig.
5
:12) to SHORT and zero the
recorder with the recorder zero control.
Set the range selector to 2 and adjust the recorder baseline with
the baseline adjust (Fig.
5
:14).
Set the range selector to the appropriate range and readjust the
recorder baseline with the baseline adjust (Fig.
5
:14). Only
minor adjustment should be necessary.
Switch AU to %T (Fig.
5
:13).
Set the range selector (Fig.
5
:12) to SHORT and zero the
recorder.
Turn the baseline adjust (Fig.
5
:14) fully clockwise.
Set the range selector so that the recorder gives a deflection just
greater than 100%.
Adjust baseline to bring the pen back to 100%.
Exchange the converter for the shutter and zero the recorder
with the recorder zero. This response will correspond to 0%
transmission.
Replace the shutter with the converter. This response will
correspond to 100% transmission. Slight adjustment of baseline
may be required to obtain the response obtained in Step
5
.
On completion of the chromatographic run
,
flush the flow cell
either with pure solvent or the buffer used in the
chromatographic run. To prevent the deposition of salts from
aqueous buffers
,
flush the cell with distilled water after use
,
if
necessary after disconnecting the column.
Note:
Never allow aqueous buffers to dry out in the cell. Either
continue to flush through with buffer or leave the cell filled
with distilled water.
2. Leave the UV-1 switched on. The monitor should only be
switched off if it is not going to be used again for more than one
week.
17
