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8. Accessories and Spare Parts

8. Accessories and

Spare Parts

Please order accessories and spare parts according to the designation
and code numbers given below.

Designation

Filters

Filter kit, 254 nm

Filter kit, 280 nm

Filter kit, 405 nm

Aperture

Converter 280 nm

Flow cells

Flow cell S-2 complete

with measuring cell

Flow cell 10 mm complete

with measuring cell

Flow cell 3 mm

with measuring cell

Flow cell HR-10

with measuring cell

Flow cell large volume

with measuring cell

Measuring cell, 3 mm

Measuring cell, 10 mm

Accessories and Spare parts

UV lamp complete

Tubing and fittings

Tubing, PTFE, (pack of 5 m)

Shutter

Allen key

Signal input cable

Mains cable, US

Mains cable, EU

Fuse holder for 125 mA fuse

Fuse holder for 250 mA fuse

Fuse, 125 mA for 220 V

Fuse, 250 mA for 110 V

Code No.

19-2432-01

19-2433-01

19-4724-01

19-2492-01

19-2486-01

19-4840-02

19-2504-02

19-2503-02

19-6254-02

19-4510-02

19-2525-01

19-2524-01

19-3807-01

19-2505-01

19-0041-01

19-2491-01

19-0379-01

19-2853-01

19-2447-01

19-2448-01

19-2925-01

19-2926-01

19-2367-01

19-2368-01

Pieces

Содержание UV-1

Страница 1: ...Monitor UV 1 User Manual Edition AG 59 7797 01 ...

Страница 2: ...in the conditions of delivery is valid only if the product has been installed and used according to the instructions supplied by GE Healthcare Bio Sciences AB GE Healthcare Bio Sciences AB shall in no event be liable for incidental or consequential damages including without limitation lost profits loss of income loss of business opportunities loss of use and other related exposures however caused ...

Страница 3: ...AU or transmission T 15 4 3 Conversion table T to AU and OD 16 4 4 Start up 16 4 5 Stabilization time 16 4 6 Basic operating procedure AU 17 4 7 Basic operating procedure Transmission T 17 4 8 Shut down 17 5 Maintenance 18 5 1 General precautions 18 5 2 Cleaning the flow cell 18 5 3 Changing the flow cell 19 5 4 Interference filters 20 5 5 Other optical surfaces 20 5 6 Instrument housing 20 5 7 La...

Страница 4: ...able light source furthermore a built in reference cell eliminates baseline drift The output signal can be recor ded in either AU or T Sensitivity range between 0 01 and 2 AUFS or 0 100 T The UV 1 offers a range of three detection wavelengths 254 280 and 405 nm In addition five flow cells are available for different applications in Standard Chromatography FPLC and Industrial applications 4 ...

Страница 5: ...ing upon the flow cell chosen Note Flow cell S 2 has only the sample cell The sample cell and reference cell are angled to receive light from the same point in the lamp Fig 1 thus assuring a stable base line by negating the effects of variations in lamp intensity The lamp is powered by a stabilized DC to AC converter operating at 20 kHz to maximize its efficiency Lamp output is independent of vari...

Страница 6: ... unit If transmission is to be monitored the signal passes directly to the low pass filter If AU is to be monitored the reference cell light intensity IR is compared with the sample cell intensity IS to form log IR IS in a logarithmic circuit before passing to the low pass filter The signal finally passes to the range selector before being presented to the output terminal 6 ...

Страница 7: ...cell holder is removed by turning the locking Fig 4 6 knob on the rear panel 2 Sample inlet Inlet for sample flow 3 Sample outlet Outlet for sample flow 4 Reference inlet Inlet for flowing reference liquid The reference cell may be operated dry with static reference liquid 5 Reference outlet Outlet for flowing reference liquid 7 1 2 3 4 5 ...

Страница 8: ...ith the arrowhead next to the appropriate opening Filters converters and apertures must be pressed fully 9 Support rod Enables the optical unit to be mounted on laboratory scaffolding The rod may be secured in either of two positions by a screw and a guide pin 10 Support rod Alternative position for support rod 11 Multi core cable Connects the optical unit to the control unit via an 11 pin plug wi...

Страница 9: ...d When the UV 1 is used in the AU mode each of the other position corresponds to the optical absorbance which will give a full scale deflection fsd on a recorder with a sensitivity of 10 mV Absorbances are measured relative to air or a liquid which is present in the reference cell When the recorder is used together with UV 1 in the T mode the absorbance range selector switch is used together with ...

Страница 10: ...ses 1 x 250 mA for 110 130 V 60 Hz 1 x 125 mA for 220 240 V 50 Hz The output signal is a 10 mV DC signal The terminals are connected to a potentiometric recorder via shielded cables supplied The UV 1 is normally earthed via the mains ground Inlet for the mains cable Inlet for the optical unit To connect the shield of an output signal cable Note Do not attempt to ground the control unit via the rec...

Страница 11: ... of these items they should remain in their packing materials until required for use Carefully unpack the UV 1 Check the contents against the packing list supplied Inspect for any damage that may have occurred during transit Report any damage immediately to the local GE Healthcare representative and to the transport company concerned Save the packing material if future transport can be foreseen 3 ...

Страница 12: ...ce The filters should not allowed to come in contact with any liquid or exposed to temperatures above 60 C For directions on cleaning interference filters see Section 5 4 1 Select the appropriate filter for the wavelength to be used 2 Insert the filter and converter or aperture in the optical unit Fig 4 7 Fig 4 8 Each filter is marked with its wavelength and the letter F The 280 nm converter is ma...

Страница 13: ...matography 10 mm Fluoro plastic 10 mm 250 µl 8 7 µl 1 0 MPa Standard 19 2504 02 optical quartz 10 bar Chromatography 5 mm Silicon rubber 5 mm 0 2 MPa Industrial scale Industrial polypropylene 2 bar 19 4510 02 optical quartz Flow rate 300 I h at 0 1 MPa All flow cell are mounted in the holders ready to be installed directly into the cell housing Release the locking knob Fig 4 6 by turning it agains...

Страница 14: ...the back of the control unit Fig 6 21 The plug has a snap lock To remove it squeeze the ribbed sides firmly and pull Connecting a There is one 10 mV signal output port on the rear panel of the recorder control unit It is for use with GE Healthcare recorders or similar instruments Connect the output terminals to the input of the recorder using a signal cable Fig 12 Connect the shield of the signal ...

Страница 15: ...ither the absorbance or transmission transmittance of a flowing liquid Absorbance measurements give T recorder responses which are proportional to the solute concentration when Lambert Beers Law is obeyed The AU setting is thus most appropriate for general use particularly when quantitative results are required However even with the 3 mm path length cell there is always a slight risk that the peak...

Страница 16: ...ly installed and that the sample cell is filled with the appropriate eluent The reference cell may be left closed with air in the cell unless the UV 1 is used with eluents showing appreciable UV absorption In this case the reference cell should be filled with eluent If the instrument has been switched off turn on the mains switch Fig 5 16 and refer to Section 4 5 to restabilize the instrument Chec...

Страница 17: ...than 100 Adjust baseline to bring the pen back to 100 Exchange the converter for the shutter and zero the recorder with the recorder zero This response will correspond to 0 transmission Replace the shutter with the converter This response will correspond to 100 transmission Slight adjustment of baseline may be required to obtain the response obtained in Step 5 On completion of the chromatographic ...

Страница 18: ...e of the flow cell The cells may be inspected for particles by removing the cell holder and examining the light paths with a magnifying glass If the cell contains trapped particles proceed as follows 1 Remove the cell holder from the optical unit 2 Connect a syringe to the outlet tubing and squirt a clean solution of ethanol in distilled water 50 v v through the cell in small aliquots Examine the ...

Страница 19: ... the syringe 4 Allow the acid to remain in the cell for 10 20 minutes Longer exposures several hours will not harm the cell 5 Eject the cleaning solution carefully without splashing and rinse the cell with a distilled water 100 ml b ethanol distilled water 50 v v 100 ml c distilled water 100 ml 6 Replace the cell in the optical unit and reconnect the system to be monitored 5 3 Changing the To chan...

Страница 20: ...he UV 1 or its box Interference filters should never be exposed to temperatures above 60 C Clean all other optical surfaces by wiping the surface with clean lint free cloth moistened with carbon tetrachloride ethanol or another suitable pure solvent Wipe the instrument regularly with a damp cloth Let the instrument dry completely before use Before performing this test see that the filter is clean ...

Страница 21: ...mp carry out the tests described in Section 5 6 If the tests indicates an aging lamp proceed as follows l Disconnect the control unit from the mains supply and disconnect the optical unit from the control unit Warning The control unit must be disconnected from the mains and from the optical unit If the UV Iamp is broken make sure that all mercury is removed 2 Remove the cell holder 3 Remove the tw...

Страница 22: ... Connect the optical unit to the control unit and plug into the mains supply Switch on the instrument and allow to warm up for about 10 minutes Insert a filter and the corresponding converter or aperture Connect the control unit to a recorder with a full scale response of 50 mV Set the range selector to SHORT and zero the recorder Set AU T to T and turn the baseline adjust fully clockwise Select a...

Страница 23: ... 2 3 4 5 6 7 8 8 9 Poor ground contact Excess noise on mains supply Recorder connections incorrect Recorder range incorrect Dirty cell Deposits on optical surfaces Solvent with high UV absorption Lamp not warmed up Bubbles passing through the cell Aging lamp Check contact to ground Use alternative power source or remove source of disturbance Check connection between output terminals and recorder S...

Страница 24: ...U h at 280 nm constant temperature after 2 hours warm up 1 5 s to 90 of FSD at all ranges 0 10 mV 0 to 40 C 20 95 relative humidity 84 106 kPa 840 1060 mbar atmospheric pressure 20 VA 100 120 220 230 240 V 50 60 Hz Control unit 180x145x75 mm Optical unit 180x145x75 mm Control unit 1 6 kg Optical unit 1 7 kg EMC standards This product meets the requirement of the EMC Directive 89 336 EEC through th...

Страница 25: ...ume with measuring cell Measuring cell 3 mm Measuring cell 10 mm Accessories and Spare parts UV lamp complete Tubing and fittings Tubing PTFE pack of 5 m Shutter Allen key Signal input cable Mains cable US Mains cable EU Fuse holder for 125 mA fuse Fuse holder for 250 mA fuse Fuse 125 mA for 220 V Fuse 250 mA for 110 V Code No 19 2432 01 19 2433 01 19 4724 01 19 2492 01 19 2486 01 19 4840 02 19 25...

Страница 26: ...Printed in Sweden by SNITS DESIGN AB Västra Aros Tryckeri Nov 1996 ...

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