Action
Step
To aid in loading samples, wet the well-locating decal and apply it to the
front of the glass plate so that the appropriate edge outlines the sample
wells.
Note:
The side wells for standards of a preparative comb correspond to the outer-
most wells formed by the 10-well comb.
1
Fill the sample wells and each upper buffer chamber that will be used with
running buffer. One upper buffer chamber holds approximately 75 mL.
2
Load the sample into the wells using a fine-tipped microsyringe. The width
of the wells depends on the number of wells per comb.For the volume re-
quired to fill to 1mm well depth, see the table below.
3
Volume of sample (µL) per 1 mm depth
Comb thickness (mm)
No. of wells
1.5
1.0
0.75
19.1
12.7
9.5
5
5.8
9
7.2
4.8
3.6
10
4.4
2.9
2.2
15
2.9
18
Final assembly
CAUTION
Devices must only be operated with a certified power supply capa-
ble of limiting the voltage and current to specified ratings.
38
SE 250/260 Mighty Small II Operating Instructions 29281629 AA
5 Operation
5.2 Assembly
