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Electrophoresis Power Supply EPS 3501 XL

Operating Instructions

28-9639-69

AA

41

Maintenance 5

General 5.1

5 Maintenance

5.1 General

No user maintenance is required for Electrophoresis Power Supply EPS 3501 XL.

NOTICE
Cleaning

. Keep the instrument dry and clean. Wipe regularly with a soft

damp tissue and, if necessary, a mild cleaning agent. Let the instrument
dry completely before use.

WARNING
Electrical shock hazard

. All repairs should be done by service

personnel authorized by GE Healthcare. Do not open any covers or
replace parts unless specifically stated in the user documentation.

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Содержание EPS 3501 XL

Страница 1: ...GE Healthcare Electrophoresis Power Supply EPS 3501 XL Operating Instructions Original instructions...

Страница 2: ......

Страница 3: ...eatures 20 2 5 Recycling information 21 3 Installation 23 3 1 Site requirements 23 3 2 Unpacking 23 3 3 Transport 23 3 4 Connections 24 3 5 Spare parts and accessories 25 4 Operation 27 4 1 Operation...

Страница 4: ...Table of Contents 4 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA...

Страница 5: ...nderstand the concepts of electrophoresis You must read the Safety Instructions in Chapter 2 of these Operating Instructions The system should be installed according to the instructions in Chapter 3 o...

Страница 6: ...ting Instructions contain WARNINGS CAUTIONS and NOTICES concerning the use of the product with meanings as defined below WARNING WARNING indicates a hazardous situation which if not avoided could resu...

Страница 7: ...s e g File Open refers to the Open option in the File menu 1 2 Regulatory information This section lists the directives and standards that are fulfilled by EPS 3501 XL Manufacturing information CE Con...

Страница 8: ...lthcare Regulatory compliance of connected equipment Any equipment connected to EPS 3501 XL should meet the safety requirements of EN 61010 1 IEC61010 1 or relevant harmonized standards Within the Eur...

Страница 9: ...electric focusing using Immobiline or carrier ampholytes such as CleanGel and ExcelGel Flatbed SDS PAGE Polyacrylamide Gel Electrophoresis or flatbed native PAGE EPS 3501 XL is also suitable for DNA s...

Страница 10: ...ment 10 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA Instrument description Figure 1 1 The main parts of the EPS 3501 XL instrument 1 3 2 Part Description 1 Display 2...

Страница 11: ...tage is applied HV on and connectors for two electrophoresis units Display Indicator Switch Color Description HV ON indicator lamp Green The HV ON lamp indicates that the high voltage power supply is...

Страница 12: ...n time and volthours in RUN PAUSE and END The values scroll i e they automatically change from maximum to minimum value or vice versa run Press run to start the run and put the program into RUN mode T...

Страница 13: ...am in MORE mode This gives access to some special functions These include COPY Copying a program CLEAR Clearing a program SETUP Disabling the start current check MORE mode cannot be activated in RUN o...

Страница 14: ...1 Introduction 1 3 Instrument 14 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA...

Страница 15: ...g the equipment you must be aware of the hazards described in this manual Follow the instructions provided to avoid personal injury or damage to the equipment The safety precautions in this section ar...

Страница 16: ...performed by properly trained personnel only WARNING EPS 3501 XL is a high voltage instrument that can cause fatal electrical shock if the safety features are disabled The safety lid must be securely...

Страница 17: ...power cord must always be easy to disconnect WARNING Hazardous biological agents during run When using hazardous biological agents flush the entire system tubing with bacteriostatic solution followed...

Страница 18: ...sed in safety labels 28 9269 30 ETTAN IPGphor 3 Made in Sweden 28952336aa GE Healthcare Bio Sciences AB 751 84 Uppsala Sweden Serial No 1234567 N12406 Code No 11003364 Mfg Year 2009 Voltage 100 240 V...

Страница 19: ...le European directives Label Meaning Label Meaning This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected se...

Страница 20: ...required disconnect the mains power cord The run is interrupted immediately Power failure to will result in EPS 3501 XL The run is interrupted immediately in an undefined state Function Description F...

Страница 21: ...501 XL contains hazardous substances Detailed information is available from your GE Healthcare representative Disposal of electrical components Waste of electrical and electronic equipment must not be...

Страница 22: ...2 Safety instructions 2 5 Recycling information 22 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA...

Страница 23: ...on see Electrophoresis Power Supply EPS 3501 XL User Manual 3 1 Site requirements 3 2 Unpacking Check the equipment for damage before starting assembly and installation Document any damage and contact...

Страница 24: ...nel Local regulation for Great Britain This appliance must be earthed The wires in the mains lead are colored in accordance with the following code If the plug provided is unsuitable for your socket o...

Страница 25: ...d black The brown wire must be connected to the terminal which is marked with the letter L or colored red Note After replacing or changing a fuse the fuse cover in the plug must be replaced with a fus...

Страница 26: ...3 Installation 3 5 Spare parts and accessories 26 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA...

Страница 27: ...racters in the display are shown as bold characters in this manual 4 2 Starting the instrument Turn on the instrument by pressing the Power switch to the I position When the power supply is turned on...

Страница 28: ...h 1 1 enter set 2 1 2 1 0 mA 0 W 2 1 0 mA 0 W 1 mA 5 W 0 00 h 2 1 enter set enter set SET 1000 V 10 mA 0 W 0 00 h 2 1 enter set enter set Vh SET 1000 V 10 mA 10 W 0 00 h 2 1 SET 1000 V 10 mA 10 W 0 V...

Страница 29: ...led by one of these limiting values which means that it is run at either constant voltage current or power The EPS 3501 XL automatically switches over the controlling parameter according to programmed...

Страница 30: ...a step program Choosing gradient means that a voltage endpoint for the actual phase is programmed together with current and power limiting values A linear voltage gradient is made with zero for the f...

Страница 31: ...radient programming the programming for the IEF part of a 2 D electrophoresis with Immobiline DryStrip Gels is shown in Table 4 2 and Figure 4 3 Note that the first phase is a very steep gradient to r...

Страница 32: ...or manual break choose OFF with Confirm with set enter Note that the breakpoint unit is valid for all phases within the program and you will only get this question when programming the first phase see...

Страница 33: ...with the program number flashing It is possible to go back to this position at any stage during programming in SET mode by pressing exit or STOP Note that the program is automatically saved with all c...

Страница 34: ...e is done by entering the last phase as above moving to the question Last Phase and answering No N The program will jump to the start position for programming a phase with the phase number blinking Pr...

Страница 35: ...Choosing a program Press set enter and select the program you wish to run by pressing until the value is correct Omit this step if you have just programmed or edited a method as described in Programmi...

Страница 36: ...Insert delete 4 Choose Insert or Delete 5a Program a new phase 2 5b Back to new Phase 2 start position Old phase 2 is deleted step GRADIENT enter set A 500 V 1 mA 5 W SET 1 1 1 Vh SET 2 1 SET 2 1 100...

Страница 37: ...omplete press either pause continue again or run to continue the run from where it was interrupted Editing a running program when in PAUSE When in the PAUSE mode you can also press set enter to make c...

Страница 38: ...umber 2 Start the run 3 During a run you can view the settings It is also possible to interrupt the run and make changes in the program 4 Stop the run manually 5 The run is stopped automatically 1000...

Страница 39: ...o to zero as indicated by the HV on LED switching off The end parameter values are displayed Switch between total elapsed time integrated voltage or integrated current for all phases in the program by...

Страница 40: ...4 Operation 4 5 Performing a run 40 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA...

Страница 41: ...upply EPS 3501 XL NOTICE Cleaning Keep the instrument dry and clean Wipe regularly with a soft damp tissue and if necessary a mild cleaning agent Let the instrument dry completely before use WARNING E...

Страница 42: ...5 Maintenance 5 1 General 42 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA...

Страница 43: ...Cause Action The current is less than the lower limit This can be due to incorrect connection of the electrophoresis equipment or due to use of buffers with extremely low conductivity 1 Check connecti...

Страница 44: ...6 Troubleshooting 44 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA...

Страница 45: ...XL refer to the Electrophoresis Power Supply EPS 3501 XL User Manual 7 3 Ordering information For ordering information visit www gelifesciences com 2DE Parameter Value Ingression protection IP20 Supp...

Страница 46: ...7 Reference information 7 3 Ordering information 46 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA...

Страница 47: ...al and communication connections Appendix A Appendix A Electrical and communication connections Voltage 100 120 220 240 V AC Frequency 50 60 Hz Power 260 W 4 AMax 100 120 220 240 MAINS Disconnectbefor...

Страница 48: ...Appendix A Electrical and communication connections 48 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA...

Страница 49: ......

Страница 50: ...009 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Con...

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