•
If applicable, clean the pH electrode manually and make sure to leave it in an appro-
priate buffer. See ÄKTA pure User Manual for detailed instructions.
System storage
If the instrument is not going to be used for a couple of days or longer, also perform the
following:
•
Fill the system and inlets with storage solution (e.g., 20% ethanol) using System CIP.
If Column valve V9-C or V9H-C is mounted, the integrated pressure sensor
of the valve allows the system to monitor the post-column pressure. The
limit for the pressure sensor in Column valve V9-C or V9H-C is automati-
cally set so that the UV monitor and the pH monitor are protected from
high pressure. If Column valve V9-C or V9H-C is not mounted, make sure
to keep the pressure in the system after the column below the pressure
limits for the modules in the flow path.
Note:
•
If applicable, prepare the pH electrode for storage as described in ÄKTA pure User
Manual.
Column cleaning
After a method run is completed, perform the following:
•
Clean the column with one or several cleaning solution(s) using Column CIP.
Column storage
If the column is not going to be used for a couple of days or longer, also perform the
following:
•
Fill the column with storage solution (e.g., 20% ethanol) using Column CIP.
pH electrode storage
If pH monitoring will not be used for a week or longer, perform one of the following ac-
tions:
•
Inject new storage solution into the pH flow cell.
•
Replace the pH electrode with the dummy electrode that is installed in the pH valve
on delivery.
ÄKTA pure Operating Instructions 29022997 AF
129
6 Run a method
6.5 After run procedures
