GE HEALTHCARE Biacore T100, Руководство пользователя

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Biacore T100 Software Handbook BR-1006-48 Edition AE
141
Concentration analysis 8
The left-hand panel lists the details of the calibration curve data, with
concentration and response or slope values for all calibration points. The
Calc
conc column lists the concentration corresponding to the actual response value
as determined from the fitted calibration curve. Select a row in the table to
highlight the corresponding point on the calibration curve.
The calibration curves are shown in the right-hand panel. Choose the curve to
display from the list at the top of the panel. Each calibration curve is constructed
from the cycles in an assay step with the purpose
Calibration . Right-click on a
curve or calibration point for options for excluding calibration curves or single
cycles from the evaluation. Excluded cycles are shown as open symbols and
excluded curves are shown as broken lines. The excluded points are marked in
the left-hand panel with red strikethrough text. You can also choose to display
sensorgrams for individual calibration cycles from the right-click menu.
Note: The sample name entered for the calibration samples is used only as a
title for the calibration curve. For wizard-based runs, the sample name
will be the same for all calibration cycles, but different names can be
introduced either in method-based runs or by editing the keyword table.
Changing sample names in calibration cycles only changes the title in the
calibration curve panel and has no effect on the curve itself.
Flow cell Concentration analysis may be performed without a refer-
ence cell, since the unknown samples are determined by
direct reference to a calibration curve obtained under the
same conditions. If a reference cell is included in the flow
path for the run, the reference-subtracted curve may how-
ever be selected if desired.
Report point Response levels for concentration analysis (relative
response) are normally taken from a report point shortly
after the end of the sample injection, to avoid contributions
from the bulk refractive index of the sample. For analyses
based on the rate of binding (report point slope), a report
point early in the sample injection is normally used.
Response type Choose between relative response and slope.
Fitting function Choose between linear and 4-parameter functions, or
custom models if you have defined any (Section 8.2.4).
The 4-parameter function is a general fitting function for
continuous curves, and is recommended for most purposes.
Use a linear function only if you have good reason to expect
the calibration curve to be a straight line. See Section 7.2.4
for the equations for fitting functions.