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FLUORESCENT IMAGE ANALYZER

FLA-5000

Operation Guide

Science System, Industrial Materials Dept.

Fuji Photo Film Co., Ltd.

Version 1.0 October 2001

Содержание FLA-5000

Страница 1: ...1 FLUORESCENT IMAGEANALYZER FLA 5000 Operation Guide Science System Industrial Materials Dept Fuji Photo Film Co Ltd Version 1 0 October 2001 ...

Страница 2: ... 16 8 Reading digitize Samples 19 Index Thank you for purchasing our fluorescent image analyzer Model FLA 5000 This Operator s Guide is intended to provide a brief description of the operating procedures for the image analyzer unit incor porated into your system together with the functional options available from the unit For a detailed description of the opera tion of the unit please refer to the...

Страница 3: ...ic range reliable linearity high resolution and capability of reuse Ensures high resolution high sharpness and linearity not attainable by existing filmless systems and Obviates the need for a darkroom and automated processing lab for configuration of the system 2 Turning ON OFF the FLA 5000 1 Press the POWER switch on the right side of the unit to the I position 2 The POWER indicator lamp should ...

Страница 4: ...eading conditions Chemiluminescencemode Click this button to open the screen for setting the chemiluminescentsample reading conditions Digitize mode Click this button to open the screen for setting the digitize sample reading conditions Use this button to recall stored reading conditions Use this button to change or register the filter Use this button to set the display menu and file for mat The c...

Страница 5: ...opriate material Never use a water dampened cloth For any heavy contamination use a cloth with a small amount of anhydrous ethanol Extra Pure or Guaranteed Reagent In actuality however it is necessary to ensure that such appropriate anhydrous ethanol to be used has been kept in a brown re agent bottle or stored in the proper environment recommended by the maker since any anhy drous ethanol stored ...

Страница 6: ...elow 1 File Name Assign a file name to the read image data for saving 2 Comments Enter any comments required for saving with the assigned file name 3 Change Filter Use this button when changing any filter in formation No need to use this option in the normal IP reading operation 4 Laser Select the laser to be used In the IP reading mode the 635nm laser will be automatically selected 5 Filter Selec...

Страница 7: ...e is the higher the voltage to be applied to the PMT and the reading sensitivity are Small Value Large Low Sensitivity High 6 Place the IP on the IP stage You may use only IPs with magnetic absorption layers on the back on the IP stage Since an IP is stuck on the IP stage by the magnetic force any IPs without magnetic absorption layers may not be attached to the IP stage Such IPs are unusable 6 1 ...

Страница 8: ...8 1 Click the button and reading starts 7 Load the IP stage into the FLA 5000 body 7 1 Open the door of the stage setting block and set the IP stage with the stuck IP facedown 7 2 Push the IP stage to the very end until it butts 7 3 Close the door of the stage setting block 8 2 When reading finishes normally the following dialog box appears Click the button 8 3 You may finish reading at any time b...

Страница 9: ...er tubes PMTs built in the FLA 5000 as shown below One PMT is built in the FLA 5000 Two reading modes are available Two PMTs are built in the FLA 5000 Six reading modes are available Available when two PMTs are built in A v a i l a b l e when one PMT is built in 1 laser 1 image One laser is used to excite fluorescent samples Fluorescent light from a sample passes through a single filter and forms ...

Страница 10: ...10 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 1 l aser 1 i m age 1 l aser 1 i m age C ycl i c 16 17 1 l aser 2 i m age 1 l aser 2 i m age C ycl i c 18 17 2 l aser 2 i m age 2 l aser 2 i m age C ycl i c ...

Страница 11: ... Area The actual reading area will be shown in the form of a red colored box As necessary move the cursor onto the red frame or to any point in the red box and drag the box to change the size and or position of the reading area 12 Read Click on this button to start the reading operation 13 Save Template Use this button to save the reading conditions in a file For details see the Operation Manual 1...

Страница 12: ... stage then as shown below This is an easy way 6 1 1 Open the door of the stage setting block and set the fluorescent sample stage with the printed frame side faceup on the FLA 5000 6 1 2 Set the gel stopper as shown below 6 1 3 Put a gel sample so that the side with charging holes faces up Fix it with the gel stopper Set the stage in the front position Do not pull it to the very end 6 1 4 Press t...

Страница 13: ... 6 2 1 Set a tighter plate in any position of the tighter plate frame set on the multi stage 6 2 2 Open the door of the stage setting block and set the multi stage with the printed frame side faceup on the FLA 5000 Push the multi stage to the very end 6 2 3 Close the door of the stage setting block ...

Страница 14: ...o lock them 6 3 4 Fix the glass with two glass holders in the front position and one glass holder in the rear position Turn the dial of each glass holder clockwise to fix the glass There are spacers on the side of the glass holders Each spacer has two levels Slide the spacers to adjust the balance of the glass holders Movable stays 6 3 5 Open the door of the stage setting block and set the multi s...

Страница 15: ...set reading area where reading is completed is displayed in the reading status real time display window Reading is executed from the front side of the stage to the back On the monitor screen read data is displayed from below to above 7 2 When reading finishes normally the following dialog box appears Click the button 7 3 You may finish reading at any time before the whole reading area is read Clic...

Страница 16: ...his option in the normal chemiluminescent sample reading operation 4 Enter the voltage value to be applied to the first PMT Voltage values must be an integer between 250 and 1000 5 Filter Select the filter to be used for reading In the chemiluminescent sample reading mode no filter is used as a general rule In the chemiluminscence mode the filter tray must contain at least one through position whe...

Страница 17: ...o the very end 7 Set the chemiluminescent sample on the fluorescent sample stage The following descriptions show an example of setting a membrane sample 7 1 Set a membrane sample with the chemiluminescent side facedown on the fluorescent sample stage Tape the corners of the membrane to reduce influences of physical vibrations upon the image 7 2 Press the fluorescent sample stage slowly to the very...

Страница 18: ... button and reading starts 8 2 When reading finishes normally the following dialog box appears Click the button 8 3 You may finish reading at any time before the whole reading area is read Click the button when you want to finish reading ...

Страница 19: ...eed to use this option in the normal chemiluminescent sample reading operation 4 Laser Select a laser unit to be used for reading from the pull down menu In the digitizing mode two options are available 473nm and 532nm 5 Enter the voltage value to be applied to the first PMT Voltage values must be an integer between 250 and 1000 6 Filter Click to select the filter to be used for reading In the dig...

Страница 20: ...the very end 7 Set the fluorescent sample stage on the FLA 5000 7 1 Set a sample with the migration side facedown on the fluorescent sample stage 7 2 Put the fluorescent plate for digitizing supplied with the fluorescent sample stage on the digitize sample Put the fluorescent plate with the mat lusterless side facedown 7 3 Press the fluorescent sample stage slowly to the very end until it butts 7 ...

Страница 21: ... 1 Click the button and reading starts 8 2 When reading finishes normally the following dialog box appears Click the button 8 3 You may finish reading at any time before the whole reading area is read Click the button when you want to finish reading ...

Страница 22: ...22 Science System Industrial Materials Dept Fuji Photo Film Co Ltd Version 1 0 October 2001 ...

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