FiT SUPER SESI, Руководство пользователя

Страница: 20 / 36

Page 18 of 34
SUPER SESI, by FIT User manual, Revision 2.0
Changing the electrospray silica capillary
CAUTION During this operation, the electrospray voltage must be set to zero, the
pressure of the electrospray vial holder must be set to zero, and the temperature of the
ionization chamber must be set below 40ºC. Once the ionization chamber set
temperature is changed, wait for at least 30 min until it cools down.
If the spray is on, set the electrospray voltage to zero. See section ‘Stopping the electrospray´
for more details.
If the MS is acquiring, set the MS to stand-by mode. Refer to the user manual of your mass
spectrometer for this.
If the vial holder is pressurized, depressurize it. For this, make sure that the syringe is not
connected to the syringe port and open the syringe valve 2 (up position).
If the ionization chamber temperature is high, set it to a safe value and wait for it to cool down.
IMPORTANT NOTE It is very important to make sure that the silica capillary, the
electrospray probe and the electrospray vial holder are free from contamination.
Always use gloves when manipulating them, and make sure that all surfaces are very
clean and free from any contamination that could affect your measurements. If these
parts get contaminated by accidental contact with the skin or with other contaminated
surface, the background of your measurements will be affected until you clean them.
Once the system is cooled, the voltage is switched off, and the vial holder is depressurized,
unlock the electrospray probe, gently pull it out from the ionization chamber, and put it on the
maintenance position.
Figure 14. Unlocking the electrospray probe .
*The figure may differ from the latest
design.
TIPS & TRICKS In order to reduce the pressure needed to pressurize the vial holder and create
the Electrospray, we recommend to use a sharp silica capillary (20um id) as short as
possible*, and an extension of silica capillary (50um id) unto the vial holder. For this, we
provide a Union fitting that is attached to the Electrospray Probe Handgrip, so the two
capillaries described previously can be connected at this point.
* This length must be long enough to reach the Fitting union (Figure 15).