8
8
ADJUSTMENT
Adjustment is done with the service tool.
1. Place the service tool into the openings of the calibration nut at the top of the handle.
2. Turn the service tool clockwise to increase, or counterclockwise to decrease the volume.
3. After adjustment check the calibration according to the instructions above.
FORMULAS FOR CALCULATING RESULTS
Conversion of mass to volume
V = (w + e) x Z
V = volume (µl)
w = weight (mg)
e = evaporation loss (mg)
Z = conversion factor for mg/µl conversion
Evaporation loss can be significant with low volumes. To determine mass loss, dispense water
to the weighing vessel, note the reading and start a stopwatch. See how much the reading
decreases during 30 seconds (e.g. 6 mg = 0.2 mg/s).
Compare this to the pipetting time from taring to reading. Typically pipetting time might be 10
seconds and the mass loss is 2 mg (10 s x 0.2 mg/s) in this example. If an evaporation trap or
lid on the vessel is used the correction of evaporation is usually unnecessary.
Range Volume Accuracy Precision
µl
µl
% s.d.µl cv%
0,2-2µl
2
±0.080 ±4
0.040 2.0
0.2
±0.080 ±40
0.020 20.0
0,5-10µl
10
±0.120 ±1.2 0.080 0.8
1
±0.120 ±12
0.080 8.0
2-20µl
20
±0.20
±1.0 0.10
0.5
2
±0.20
±10.0 0.10
5.0
5-50µl
50
±0.50
±1.0 0.20
0.4
5
±0.50
±10
0.20
4.0
10-100µl
100
±0.80
±0.8 0.30
0.3
10
±0.80
±8.0 0.30
3.0
20-200µl
200
±1.60
±0.8 0.60
0.3
20
±1.60
±8.0 0.60
3.0
200-1000µl 1000 ±8.0
±0.8 3.0
0.3
200
±8.0
±4.0 3.0
1.5
100-1000µl 1000 ±8.0
±0.8 3.0
0.3
100
±8.0
±8.0 3.0
3.0
1-5 ml
5000 ±40.0
±0.8 15.0
0.3
1000 ±40.0
±4.0 15.0
1.5
2-10 ml
10000 ±60.0
±0.6 30.0
0.3
2000 ±60.0
±3.0 30.0
1.5
Volume Accuracy Precision
µl
µl
% s.d.*µl cv%
1 ±0.04 ±4.0 0.04 4.0
2 ±0.05 ±2.5 0.04 2.0
5 ±0.07 ±1.4 0.07 1.4
10 ±0.09 ±0.9 0.08 0.8
20 ±0.12 ±0.6 0.10 0.5
25 ±0.15 ±0.6 0.13 0.5
50 ±0.3
±0.6 0.2
0.4
100 ±0.4
±0.4 0.3
0.3
200 ±0.8
±0.4 0.6
0.3
250 ±1.0
±0.4 0.8
0.3
500 ±1.5
±0.3 1.5
0.3
1000 ±3.0
±0.3 0.3
0.3
2000 ±6.0
±0.3 4.0
0.2
3000 ±9.0
±0.3 6.0
0.2
5000 ±15.0 ±0.3 10.0 0.2
10 ml ±30.0 ±0.3 20.0 0.2
TABLE 1: Maximum permissible errors according ISO8655
