6
5
6
4
Before you begin your actual pipetting work, fill and empty the tip 2-3 times with the solution
that you will be pipetting. Hold the pipette in an upright position while aspirating liquid. The
grippy should rest on your index finger. Make sure that the tips, pipette and solution are at the
same temperature.
FORWARD TECHNIQUE
Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button to the first stop.
2. Dip the tip under the surface of the liquid in the reservoir to a depth of about 1 cm and
slowly release the push button. Withdraw the tip from the liquid touching it against the
edge of the reservoir to remove excess liquid.
3. Deliver the liquid by gently depressing the push button to the first stop. After a delay of
about one second, continue to depress the push button all the way to the second stop.
This action will empty the tip.
4. Release the push button to the ready position. If necessary, change the tip and
continue pipetting.
REVERSE TECHNIQUE
The reverse technique is suitable for dispensing liquids that have a high viscosity or a tendency
to foam easily. The technique is also recommended for dispensing very small volumes. Fill a
clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the second stop.
2. Dip the tip under the surface of the liquid in the reservoir to a depth of about 1 cm, and
slowly release the push button.
This action will fill the tip. Withdraw the tip from the liquid touching it against the edge of
the reservoir to remove excess liquid.
3. Deliver the preset volume by gently depressing the push button to the first stop.
Hold the push button at the first stop. Some liquid will remain in the tip and this should not
be included in the delivery.
4. The remaining liquid should either be discarded with the tip or pipetted back into the
container.
REPETITIVE TECHNIQUE
The repetitive technique offers a rapid and simple procedure for repeated delivery of the same
volume. Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the second stop.
2. Dip the tip under the surface of the liquid in the reservoir to a depth of about 1 cm, and
slowly release the push button. This action will fill the tip. Withdraw the tip from the liquid
touching against the edge of the reservoir to remove excess liquid.
3. Deliver the preset volume by gently depressing the push button to the first stop.
Hold the push button at the first stop. Some liquid will remain in the tip and this should not
be included in the delivery.
4. Continue pipetting by repeating steps 2 and 3.
