IRB20 Inverted Microscope Trouble Shooting
10
Fein
TROUBLE
POSSIBLE CAUSE
SOLUTION
Field of view
obstructed or
irregular illumination
Nosepiece is not clicked into position.
Move nosepiece into position.
Filter slider is not in place.
Adjust filter slider.
Phase Contrast slider is not in place.
Adjust phase slider.
Dust in field of view
Objective lens is dirty.
Clean objective lens.
Sample has dirt or dust on it.
Use a new sample.
Eyepiece is dirty.
Clean eyepiece lens.
Hot spot on image
Aperture diaphragm isn’t open enough.
Adjust aperture diaphragm.
Poor image: low
contrast, image is not
sharp, image details
are not clear.
Objective is not in correct position.
Rotate nosepiece into position.
Objective lens is dirty.
Clean objective lens.
Culture dish is too thick.
Use normal size culture dish.
Sample is dirty.
Clean sample.
Part of image is not
clear.
Objective is not in correct position.
Rotate nosepiece into position.
Sample is not placed in correct place on stage.
Adjust sample and holder.
Phase contrast
observation is poor.
Annular spot did not focus with dish holder.
Readjust phase alignment.
Culture dish bottom is not flat.
Use standard culture dish.
Coarse focus knob is
too tight.
Tension is too tight.
Loosen tension adjustment ring.
Nosepiece slides
down automatically.
Tension is too loose.
Tighten tension adjustment ring.
Binocular field of view
is inconsistent.
The interpupillary distance is not set properly.
Adjust interpupillary distance.
Diopter is not set properly.
Adjust diopter.
Left eye and right eye have different eyepieces
in them.
Make sure a matching pair of
eyepieces are being used.
When using the high
power objective it is
touching the sample.
The culture dish bottom is too thick.
Use a standard culture dish.
The bulb does not
work.
No bulb is in place.
Install a bulb.
Bulb or fuse is broken.
Replace bulb or fuse.
The power cord is not plugged in or turned on. Ensure cord is plugged in and
unit is turned on.
Bulb burns out
quickly.
Improper bulb is being used.
Use M40-001 5W LED bulb only.
