Esco Labculture PLUS, Руководство пользователя, Страница: 22 / 30

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Labculture
•
PLUS
3.5 UV Lamps
The UV light is a germicide and virucide. Even at the minimum acceptable irradiance in a BSC – 40 μW/cm
2
(US
Department of Health and Human Services et. al., 2000), it would only take 12.5 minutes to reach 30,000
μW/cm2 (1 W = 1 J/sec), which has been listed as germicidal for spore forming organism.
Unlike many other type of decontamination agent, UV light doesn’t leave any residue. The decontamination
action stops upon de-energizing of the lamp.
However, due to the short wavelength, the UV light does not penetrate well. Thus UV light can only be used to
effectively disinfect the work area of an empty BSC. For any container stored inside the BSC’s work area, the
UV radiation will only disinfect the outer surface of the material, leaving the inner surface and the content
inside the material untouched.
UV light decontamination method may be used before and after working with vegetative organisms
and viruses. However, it should not be the sole decontamination agent; the use of chemical
decontamination agent is still encouraged.
Minimize the material inside the BSC’s work area during the process of UV light decontamination. A
direct interaction with UV light can degenerate plastic- or rubber-based material and can cause other
hazard.
Make sure that the BSC sash is in the fully closed position and the interlock is working properly before
activating the UV lamp. Avoid direct contact with skin and eyes as UV light is classified as a probable
human carcinogen.
The UV lamp should be activated for around 60 minutes to work effectively. Use the UV timer feature
to easily control the period of decontamination (UV timer is disabled by default). Leaving the UV lamp
on for over 60 minutes or even overnight is not recommended because it shortens the lifespan of the
lamp. The UV lamps used in Esco BSC have a lifespan of 2,000 hours.
The UV lamp should be cleaned of any dust and dirt weekly and changed annually to ensure its
effectiveness.
The use of UV lamp in BSC has been explicitly discouraged in all major international standards and
recommendations.
3.6 Decontamination and Disinfecting Agents
For stainless steel surfaces, all common disinfecting agents except chlorine-based ones are suitable.
For powder coated surfaces, all common disinfecting agents are suitable. However, the BSC has been
specifically evaluated for use with the following:
o
1N hydrochloric acid
o
1N sodium hydroxide
o
1% quaternary ammonium compound
o
5% formaldehyde
o
5,000 ppm hypochlorite
o
2% iodophor
o
5% phenol
o
70% ethyl alcohol
Depending on the contaminant involved at the time of operating the BSC, there are various other
types of disinfecting agents that may be used. The following table outlines the effectiveness of various
disinfecting agents against the different types of contaminants.