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11 

Cytotoxic Lead Shielded Class II Biological Safety Cabinets 

However, sitting position has some drawbacks too: 

 

The working area available is fairly limited 

 

There is a potential risk of being constrained in the same posture for a long time 

 

Sitting posture is one of the most stressful postures for one’s back 

Therefore you should pay careful attention to the following guidelines in order to achieve comfortable and 
healthy working conditions: 

 

Always ensure that your legs have enough legroom. 

 

Keep your lower back comfortably supported by your chair. Adjust the chair or use a pillow behind 
your back whenever necessary. 

 

You should place your feet flat on the floor or on a footrest. Don’t dangle your feet and compress 
your thighs. 

 

You should keep varying your sitting position throughout the day at regular intervals so that you are 
never in the same posture for too long. 

 

Observe the following precautions with respect to your eyes: 

o

 

Give your eyes frequent breaks. Periodically look away from the work area and focus at a 
distant point. 

o

 

Keep your glasses clean. 

 

Arrange the items/apparatus frequently used in your work in such a way that you can minimize the 
physical strain involved in handling them. 

 

Exercise regularly 

The BSC’s noise emission has been tested and found to be in compliance with EN 12469, ISO 4871 and 
NSF/ANSI 49 which is important to ensure health and comfort for the operator. 
Ergonomics accessories available with Esco include: 

 

Armrest padding 

 

Lab chair 

 

Footrest 

Please contact your local distributor or Esco for more information. 

3.5 UV Lamps 

The UV light is a very effective germicide and virucide.  Even at the minimum acceptable irradiance in a BSC – 
40 μW/cm

2

 (US Department of Health and Human Services et. al., 2000), it would only take 12.5 minutes to 

reach 30,000 μW/cm2 (1 W = 1 J/sec), which has been listed as germicidal for spore forming organism.  
Unlike many other type of decontamination agent, UV light doesn’t leave any residue. The decontamination 
action stops upon de-energizing of the lamp.  
 
However, due to the short wavelength, the UV light does not penetrate well. Thus UV light can only be used to 
effectively disinfect the work area of an empty BSC. For any container stored inside the BSC’s work area, the 
UV radiation will only disinfect the outer surface of the material, leaving the inner surface and the content 
inside the material untouched.  
 

 

UV light decontamination method may be used before and after working with vegetative organisms 
and viruses. However, it should not be the sole decontamination agent; the use of chemical 
decontamination agent is still encouraged.  

 

Minimize the material inside the BSC’s work area during the process of UV light decontamination. A 
direct interaction with UV light can degenerate plastic- or rubber-based material and can cause other 
hazard.  

 

Make sure that the BSC sash is in the fully closed position and the interlock is working properly before 
activating the UV lamp. Avoid direct contact with skin and eyes as UV light is classified as a probable 
human carcinogen. 

 

The UV lamp should be activated for around 60 minutes to work effectively. Use the UV timer feature 
to easily control the period of decontamination (UV timer is disabled by default). Leaving the UV lamp 

Содержание Cytoculture LS2-4A1

Страница 1: ...Hatboro PA 19040 USA Toll Free USA and Canada 1 877 479 3726 Tel 215 441 9661 Fax 215 441 9660 us escoglobal com usa escoglobal com Esco Micro Pte Ltd 21 Changi South Street 1 Singapore 486 777 Tel 65...

Страница 2: ...change Sentinel and Cytoculture are registered trademarks of Esco Material in this manual is provided for informational purposes only The contents and the product described in this manual including an...

Страница 3: ...tings 5 2 2 2 Calibration 6 2 2 3 Admin 7 2 2 4 Setting The Mode 7 2 3 Alarms and Warnings 9 Chapter 3 Basic Cabinet Operation 9 3 1 Sash Window Operation 9 3 1 1 Sash Window State 9 3 1 2 Using Sash...

Страница 4: ...es such as filters HEPA ULPA carbon pre filters and fluorescent UV bulbs Esco is not liable for any damage incurred on the objects used on or stored in Esco equipment If the objects are highly valuabl...

Страница 5: ...years limited for Ascent Opti 6 years for Ascent Max Cleanroom Equipment 1 year limited Laboratory Ovens and Incubators 1 year limited CO2 Incubators 2 years limited Containment Pharma Products 2 year...

Страница 6: ...in a manner not specified by this manual the protection provided by this equipment may be impaired 3 Limitation of Liability The disposal and or emission of substances used in connection with this eq...

Страница 7: ...Cytotoxic Lead Shielded Class II Biological Safety Cabinets v...

Страница 8: ...ectives 92 31 EEC The Electromagnetic Compatibility Directive and its amending directives has been designed to comply with the requirement of the following Harmonized Standard Low Voltage EN 61010 1 2...

Страница 9: ...Cytotoxic Lead Shielded Class II Biological Safety Cabinets vii...

Страница 10: ...formation 1 1 Quick View 1 Airflow Sensor 2 Hinged Front Panel 3 Sentinel Microprocessor Control System 4 Lead Glass Sash Window 5 Electrical Outlet 6 Stainless Steel Armrest 7 Access Cover to KI Impr...

Страница 11: ...The HEPA filtered air then passes through internal ducting in the back wall of the cabinet to a common air plenum where 35 is exhausted through the ULPA exhaust filter and 65 is forced evenly through...

Страница 12: ...ide one of the menu options 6 Set Mute Button o Chooses the menu or sub menu currently displayed on the LCD screen o Proceeds to the next step or sequence inside one of the menu options o Mutes the al...

Страница 13: ...1 Settings The user may use the settings menu function to customize the operation of the Cabinet to meet specific application requirements 2 2 1 1 Set Clock Time Users can set the time by increasing d...

Страница 14: ...e fan is running and also during the calibration process airflow measurement is displayed in the selected unit MENU SETTINGS VELOCITY UNIT M S FPM 2 2 1 6 Temperature Unit Selection Using this option...

Страница 15: ...s to be performed Setting the PIN to 0000 will disable this feature MENU ADMIN NEW FAN PIN XXXX 2 2 3 3 A F Monitor Whenever the air velocity falls below the fail point the air fail alarm will be trig...

Страница 16: ...normal operating height nor at fully closed position UV mode this condition can easily be corrected by putting the sash at the appropriate operation position Another warning that should be acted upon...

Страница 17: ...8...

Страница 18: ...activated whenever the sash window is moved from the normal operating height Whenever the sash window is moved to the correct height from a higher or lower position the light will automatically be tur...

Страница 19: ...aratus in the safe working area Minimize room activity personnel movements closing and opening of doors etc since these external airflow disturbances may adversely affect the BSC s internal airflow th...

Страница 20: ...tributor or Esco for more information 3 5 UV Lamps The UV light is a very effective germicide and virucide Even at the minimum acceptable irradiance in a BSC 40 W cm 2 US Department of Health and Huma...

Страница 21: ...cohol Depending on the contaminant involved at the time of operating the BSC there are various other types of disinfecting agents that may be used The following table outlines the effectiveness of var...

Страница 22: ...gen peroxide Decontamination process should only be carried out by trained personnel In any of the following eventualities the user should ensure that the BSC has been properly decontaminated keeping...

Страница 23: ...14...

Страница 24: ...to the next one 2 Hold open tubes and bottles as vertically as possible to avoid spillage Discard empty tubes and bottles into collection bags placed inside the Cabinet immediately after use 3 Use co...

Страница 25: ...wait 10 to 15 minutes Clean the spill and the area and equipment surrounding it using a suitable disinfectant 5 After the spillage has been cleaned discard your outer gloves and start using a new pair...

Страница 26: ...face of the BSC particularly on the front and top in order to remove dust that accumulated there Use clean water to finish the cleaning and wash away any residue of disinfectant agent soap water and g...

Страница 27: ...18...

Страница 28: ...APPENDIX...

Страница 29: ......

Страница 30: ...peration problems encountered etc 2 Any decontamination procedure performed by either the user or the technician should be recorded down as well 3 Please also record any major maintenance procedure pe...

Страница 31: ......

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