MIRI
®
TL family`s multiroom IVF incubators User Manual Rev. 1.0
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Each of the 14 wells is filled with approximately 25 µl culture medium. The washing wells
can also be filled (approximately with 23 µl), but it is not a requirement. The embryo is
placed at the bottom of the culturing area.
Figure 21.3
Embryo Placement in detail on the 300µ optically clear area
The process of locating the correct well is sensitive to errors. When the embryo
is positioned at the side of the well, it rests against the side. Positioning embryos
carefully to be in the center of the well circle can avoid this problem.
The identification process is easy because the wells are numbered.
Media and embryo handling are ergonomically optimized as it is possible to angle the
pipette when loading the well.
Air bubbles in the media can be easily removed by pushing them up with the
pipette tip. Air bubbles in the media will push the embryo out from the correct
position, and no time-lapse images will be generated. Usually, when air bubbles are
present, a black cloud can be seen to move around the images or cover the whole
view.
Mark the lid and the dish with the patient's name and unique identifier. It is possible to
write directly on the dish or put it on a label.
Once filled with the culture media, the culture wells must be covered by a confluent oil
layer. It is impossible to use an open culture (any culture media where a layer of oil does
not cover the culture media).
