Biometra TOne
Operation
39
}
Tap the button
ΔT
in the graphical view. Tap on the field for the step in the line un-
derneath the diagram for which you want to set a temperature increment. The step
must be within a loop.
}
Or: Tap on the field for the step in the column
ΔT
of the spreadsheet view for which
you want to set a temperature increment. The step must be within a loop.
}
Enter a temperature increment within the range of ±0.1 to 20 °C in the field
Temp.-
Incr./Decr.
.
}
Confirm the entry by tapping the green check mark.
}
Save the program after completing the changes.
ü
You have programmed a temperature increment for a selected program step. The
graphical view only displays the symbol
ΔT
(in red) in this step.
5.6.8
Programming a time increment
Certain applications, for example long-range PCR, use time increments. The holding
time is increased by the set value in each cycle.
ð
Prerequisite: You have opened an existing program or a program template for edit-
ing.
}
Tap the button
Δt
in the graphical view. Tap on the field for the step in the line un-
derneath the diagram for which you want to set a time increment. The step must be
within a loop.
}
Or: Tap on the field for the step in the column
Δt
of the spreadsheet view for which
you want to set a time increment. The step must be within a loop.
}
Enter a time increment within the range of 1 to 240 s in the field
ss
.
}
Confirm the entry by tapping the green check mark.
}
Save the program after completing the changes.
ü
You have programmed a time increment for a selected program step. The graphi-
cal view only displays the symbol
Δt
(in red) in this step.
5.6.9
Adjusting the heating and cooling rate
You can adjust the average heating and cooling rate for each individual step. This can be
useful for special PCR applications such as telomerase PCR. The average heating and
cooling rate defines how quickly the block temperature is attained in a particular step.
The maximum heating and cooling rate depends on the device model.
ð
Prerequisite: You have opened an existing program or a program template for edit-
ing.
}
Tap the button
ΔR
in the graphical view. Tap on the field for the step in the line un-
derneath the diagram for which you want to adjust the heating and cooling rate.
}
Or: Tap on the field for the step in the column
ΔR(°C/s)
of the spreadsheet view for
which you want to adjust the heating or cooling rate.
}
Use the field
°C/s
to enter an exact heating or cooling rate in increments of 0.1 °C/s.
}
As an option, you can activate the field
Apply Ramp Rate to all steps
. This applies
the heating and cooling rate to all program steps.
}
Confirm the entries by tapping the green check mark.
}
Save the program after completing the changes.
