37
SECTION 3
OPERATING THE CYTOCENTRIFUGE
3.1 Running a Cytocentrifuge Cycle
Unloading the Rotor (continued)
Cytopro Magnum chambers must remain in the rotor and in contact with the slide
for 45 seconds after cytocentrifugation has stopped. This allows the fluid to be
completely absorbed by the absorbent media. Use the lid unlock delay function to
ensure the 45-second delay is completed before opening the lid. See Section 5.1
(Troubleshooting) or the Methods Manual (RP-451) for additional information.
4
Completely depress the release lever and remove the chambers.
5
Discard used chambers and Cytopads in a biohazard container or according to
local regulations and prudent laboratory practices.
6
Remove the slides. Quickly wet fix or air dry depending on desired staining to
follow. (Wet fix for Papanicolaou, dry fix for hematology and Gram stains.)
NOTE:
If you intend to reuse standard volume chambers, you must thoroughly clean
and decontaminate them using the methods described in Section 4.2. Cytopro
Magnum chambers must be discarded after use.
Separating Chambers from Cytopads
The following information applies only to the reusable standard volume chambers.
Cytopro Magnum chambers are single use only and must be discarded after each
use.
WARNING!
This procedure requires safety protection for hands and eyes.
Before cleaning, chambers must be separated from the used Cytopads, which are
not reusable. To remove Cytopads:
1
Remove the frame from the chamber.
2 Use the sample chamber base to push the used Cytopad out of the frame and
into a biohazard container for disposal.
3 Place chambers and frames immediately into a detergent or disinfectant to
prevent cells from drying onto chamber surfaces. Sterilize chambers according
to instructions in Section 4.2.
