9
SECTION 1
INTRODUCTION
1.1 Cytopro Cytocentrifuge Overview
Table 3: Sample Treatment Options
The chart below suggests procedures for various fluids. Refer to the Methods Manual for more detailed information. Methods
currently used in other cytocentrifuges will often work in the Cytopro, if the maximum volume of fluid and the run time is adjusted
appropriately (see chart).
Sample
Prep
Cytopad
Type
Sample Vol
(mL)*
Loading
Position
Prewet
(mL)
In Situ Fix
(mL)
Speed
(rpm)
Time
(min)**
Acceleration
Hematology
CSF
e,f
Tan
0.2
Well
0-0.1
N/A
1000
3-5
High
Urine
a, d,e,f
Tan
0.2
Well
0-0.1
N/A
1000
3-5
High
Synovial
c,d,e,f
White
0.2
Well
0-0.1
N/A
1000
3-5
High
Sputum
c, e
White
0.2
Well
0-0.1
N/A
1000
3-5
High
Aspirates
a, c,d,e,f
Tan/White
0.2
Well
0-0.1
N/A
1000
3-5
High
Washes
a, d,e,f
Tan/White
0.2
Well
0-0.1
N/A
1000
3-5
High
Gram
CSF
e,f
Tan
0.2
Well
0-0.1
N/A
1000
3-5
High
Urine
a, d,e,f
Tan
0.2
Well
0-0.1
N/A
1000
3-5
High
Synovial
c,d,e,f
White
0.2
Well
0-0.1
N/A
1000
3-5
High
Sputum
c, e,f
White
0.2
Well
0-0.1
N/A
1000
3-5
High
Aspirates
a, c,d,e,f
Tan/White
0.2
Well
0-0.1
N/A
1000
3-5
High
Washes
a, d,e,f
Tan/White
0.2
Well
0-0.1
N/A
1000
3-5
High
Cytology
CSF
b, e,f,g
Tan
0.2
Well
0-0.1
Optional
1000
3-5
High
Urine
a, d,e,f,g
Tan
0.2
Well
0-0.1
Optional
1000
3-5
High
Synovial
b,c,d,e,f,g
Tan
0.2
Well
0-0.1
Optional
1000
3-5
High
Aspirates
a,b,c,d,e,f,g
Tan/White
0.2
Well
0-0.1
Optional
1000
3-5
High
Washes
a,b, d,e,f,g
Tan/White
0.2
Well
0-0.1
Optional
1000
3-5
High
Pre-Fixed
d,e,f,g
Tan
0.2
Well
0-0.1
Optional
1000
3-5
High
Cytopro
Magnum
a,b,c,d,e,f,g
N/A
2-6
Well
N/A
N/A
2000
3-10
High
LEGEND
Sample Preparation
a. Treat bloody samples.
1. Collect in anticoagulant.
2. Lyse red cells.
b. If processing will be delayed, preserve fragile cells.
c. Treat viscous samples if necessary.
d. Remove precipitates or debris when necessary.
e. Adjust cell count.
Large (epithelial) 8,000 - 12,000 per 0.2 mL sample
Medium (urothelial) 16,000 - 24,000 per 0.2 mL sample
Small (leukocytes) 50,000 - 125,000 per 0.2 mL sample
1. Concentrate low cellularity samples by
precentrifugation.
2. Dilute high cellularity samples with balanced saline
plus 2 to 4 percent bovine serum albumin (BSA).
f. Adjust cell environment where necessary.
g. Use treated slides to increase cell adhesion.
Cytopad:
† Thin samples = slow (tan).
Thick samples = fast (white).
Sample:
0.1 to 0.3 mL optimal. Samples less than 0.1 mL
yield increased volume cell loss (0.5 mL max -total fluid-
single chamber). 0.6 mL max total fluid for dual sample
chamber (2 x 0.3 mL).
Cytopro Magnum
: 2 to 6 mL optimal. Dilute smaller
samples with diluent before cytocentrifugation to
obtain at least 2 mL.
Prewetting:
† Load up to 200 µL balanced saline in
tunnel, (sample in well).
In Situ Fix:
† Load up to 200 µL of sample in tunnel,
50 to 100 µL of saccomanno type fixative in sample
well.
Speed:
High speed for small cells, low for large
and/or fragile cells.
Time:
Samples with debris, viscosity or high
cellularity will require extended run times.
* 1 drop of distilled water equals 20 to 40 µL
depending on pipette used. Other fluids may fall
outside this range.
** For samples in balanced saline, increase time up
to 2x for BSA samples and native body fluids.
† Standard volume chambers only.