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Gel-loading micropipette tips (Part Number 02-707-83) can also be purchased from Fisher
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Содержание PhysioTel Digital

Страница 1: ...Help Standard Template Error No text of specified style in document 1 PhysioTel Digital Device Surgical Manual Surgical Implantation of the PhysioTel Digital Blood Pressure and Biopotential Telemetry...

Страница 2: ...stered trademarks of 3M PhysioTel Digital Device Surgical Manual Copyright 2012 Data Sciences International All Rights Reserved Printed in U S A Part Number Rev 01 Data Sciences International DSI 119...

Страница 3: ...y personnel who will perform or assist in surgical procedures to implant PhysioTel Digital devices The surgical procedures written in this manual are at a level of detail appropriate for persons who h...

Страница 4: ...omy Approach Solid Tip ECG Lead Placement o Internal Jugular Vein o External Jugular Vein Traditional ECG Lead Placement o Lead II o Base Apex Appendix A Additional Device Information Appendix B Funct...

Страница 5: ...10 cm Sterile saline Sterile basin 2 Lidocaine Elastic vessel loops 2 0 to 4 0 Smaller or larger suture may be needed depending on size and species used non absorbable non swaged suture MES1 2 0 to 4...

Страница 6: ...tyle in document 6 Magnet Re gel syringe Vetbond Tissue adhesive if placing systemic blood pressure catheter in iliac artery Gel loading micropipette tip or insulin syringe if placing systemic blood p...

Страница 7: ...ice will require approximately 120 minutes of surgical anesthesia Intermittent positive pressure mechanical ventilation is required any time the thoracic cavity is opened such as during placement of a...

Страница 8: ...mplantation see Figure 1 Figure 1 PhysioTel Digital Device MES6 The PhysioTel Digital device measures pressure a biopotential signal temperature and physical activity in primates dogs and swine and is...

Страница 9: ...it is actually inserted into the desired vessel Must be removed prior to catheter insertion Systemic blood pressure catheter containing a radio opaque ring encircling the distal end of the systemic bl...

Страница 10: ...uitable for the biopotential signal to be monitored The negative clear lead has a solid tip and is NOT meant to be cut unless you require traditional lead placement It is designed to be introduced int...

Страница 11: ...ckage cover from the clear plastic tray Do not discard the white package cover as it contains important device calibration information Also doDo not discard the sterile package as it can be used for e...

Страница 12: ...s has been removed is recommended b Begin each scrub in the center of the scrubbed area over the planned incision site and scrub in a bulls eye pattern toward the periphery never going back to the cen...

Страница 13: ...subcutaneously along the dorsum or the flank Subcutaneous placement is not generally recommended in pigs and NHPs since they are prone to picking and rubbing the device when it is placed in this loca...

Страница 14: ...el with the sharp edge facing externally to extend the incision Keep The length of the incision in the body wall should be lesssmaller than the skin incision to allow for closure see figure 4 Figure 5...

Страница 15: ...that it will not interfere with healing once the incision is closed 6a Close the subperitoneal pocket using absorbable suture material in a simple continuous pattern 6b N A 7 Do NOT secure the antenna...

Страница 16: ...taneous device placement is appropriate in laboratory animals where the anatomy allows for a sufficiently sized pocket in the flank paralumbar fossa area in which the device and antenna can lie flat a...

Страница 17: ...routed to their implantation sites The first step to do this requires a skin incision be made over the planned implantation site i e left jugular furrow for negative solid tip lead medial thigh for me...

Страница 18: ...tenna will extend to 2a Place a small stab incision through the skin at the level that the antenna will extend to 3a Blunt dissection between muscles is used to create a pocket for the device The pock...

Страница 19: ...the thoracic cavity especially in swine due to the anatomical difference of a wider rib Figure 12 Trans diaphragmatic Approach to Left Ventricle MES12 Figure 13 Thoracotomy Approach to Left Ventricle...

Страница 20: ...40 Device on our website www datasci com A video clip of this procedure is also available on our website 5 Tie a piece of non absorbable suture around the suture aid on the catheter see Figure 14 The...

Страница 21: ...ion forceps or gently using the hand 10 Insert the tip of the catheter into the perforation in the heart wall Advance the catheter until the suture aid suture on the catheter is in direct contact with...

Страница 22: ...entricular pressure signal 12 Once proper positioning is verified draw the purse string suture closed around the catheter Ensure this suture is tight and multiple square knots are tied to prevent the...

Страница 23: ...oning when the animal is awake or in sternal recumbency Keep in mind that the heart has shifted dorsally during surgical recumbency 17b Prior to closing the thorax a 12 20 French catheter should be pl...

Страница 24: ...drawn from the chest until negative pressure is achieved Be sure to monitor the animal carefully as it is weaned from ventilator support and begins breathing on its own If it experiences difficulty br...

Страница 25: ...accessable A skin incision should be made over the palpable pulse pulling the skin to the side to avoid damaging the underlying vessel 1c The femoral artery originates deeper between muscle bellies To...

Страница 26: ...angle see Figure 22 This will be used to puncture the vessel and can be used to introduce the catheter Figure 22 Bending Needle for Catheter Placement 5 Place a length marking suture around the cathe...

Страница 27: ...he artery using the needle and insert the catheter under the tip of the needle as it is withdrawn Alternatively a vessel pick may be used to dilate the vessel slightly before placing the catheter see...

Страница 28: ...ne tipped curved forceps carefully isolate at least 2 5 cm of the iliac artery from the surrounding tissue and the iliac vein 3 Pass two elastic vessel loops or two pieces of non absorbable suture und...

Страница 29: ...ture and then stop Now release tension on the proximal occlusion suture temporarily to pass the catheter beyond this point and then replace gentle tension Continue passing the catheter until the lengt...

Страница 30: ...catheter insertion apply a Satinsky clamp to the area around the purse string to temporarily occlude blood flow This clamp should remain in place for the minimum amount of time possible so be sure ev...

Страница 31: ...abdominal device placement etc Regardless of the site chosen for implantation the basic technique is the same and will be described below 1a If the positive ECG lead will be placed on the diaphragm it...

Страница 32: ...er placement 2 Cut the lead to the appropriate length to reach the incision allowing for growth if needed Next make a circumferential cut around the silicone covering from the last few centimeters of...

Страница 33: ...is recommended in non human primates External Jugular Vein Internal Jugular Vein 1a A skin incision should be made in the jugular furrow to expose the external jugular vein approximately 4 cm caudal...

Страница 34: ...igure 35 Sutures Placed for Solid Tip Negative ECG Lead Placement 3 Tension should then be placed on the caudal suture to temporarily occlude blood flow and on the cranial ligation suture to hold the...

Страница 35: ...position to ensure the ECG signal will remain consistent once the animal is awake Once you are satisfied with the signal you can tighten the two caudal sutures around the vessel containing the lead an...

Страница 36: ...ut the device cannot be reused Clean and sterilize the device with an approved enzyme detergent and sterilant before returning the device to DSI For complete information on products and techniques app...

Страница 37: ...ice Weight Volume Height Width Length Usable Catheter Length 35 cm Catheter Diameter Temperature Range Pressure Range Initial Accuracy Battery Life Intended Cage Size Standard catheter length Also ava...

Страница 38: ...rage Storage of New Devices Carefully examine all devices when they arrive at your facility Remove the packages containing the devices from the shipping boxes Save the shipping boxes to use when retur...

Страница 39: ...it Do not store devices in saline or other liquid Sterilization before storage is necessary to prevent the spread of bacteria during handling The devices should be stored in a cool between 10 and 25...

Страница 40: ...5 cm 12002 14 Olsen Hegar Needle Holder 11095 09 Backhaus Towel Clamps Sterile permanent marker A hollow cannula and trocar or skin tunneling needle is also helpful to tunnel the intravenous lead sub...

Страница 41: ...rror No text of specified style in document 41 Gel loading micropipette tips Part Number 02 707 83 can also be purchased from Fisher Scientific Fisher Scientific Telephone 1 800 766 7000 Fax 1 800 926...

Страница 42: ...elect the animal icon right click and choose Start Sampling Continuous to display the Start Continuous Sampling window 4 Select Trace to display a waveform trace in the graph window It will not save t...

Страница 43: ...atus window double click on the blood pressure channel to open the Blood Pressure Analysis Attributes window Select the Offsets tab 7 Under Implant Pressure Offset click the Measure button to see an i...

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